Assessment of Colobanthus quitensis genetic polymorphism from the Argentine Islands region (maritime Antarctic) by actin, α- and γ-tubulin gene intron analysis
Colobanthus quitensis is one of the two angiosperm plant species commonly spread in the Antarctic. The species has been extensively analyzed at morphological, anatomical and physiological levels, but information regarding its genetic vari-ability remains limited. The aim of the study was to identify...
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| Format: | Article |
| Language: | English |
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State Institution National Antarctic Scientific Center
2020-07-01
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| Series: | Український антарктичний журнал |
| Subjects: | |
| Online Access: | http://uaj.uac.gov.ua/index.php/uaj/article/view/382 |
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| author | A. Rabokon A. Postovoitovа Yu. Bilonozhko L. Kalafat M. Pavlovska Ie. Prekrasna I. Parnikoza I. Kozeretska Ya. Pirko Ya. Blume |
| author_facet | A. Rabokon A. Postovoitovа Yu. Bilonozhko L. Kalafat M. Pavlovska Ie. Prekrasna I. Parnikoza I. Kozeretska Ya. Pirko Ya. Blume |
| author_sort | A. Rabokon |
| collection | DOAJ |
| description | Colobanthus quitensis is one of the two angiosperm plant species commonly spread in the Antarctic. The species has been extensively analyzed at morphological, anatomical and physiological levels, but information regarding its genetic vari-ability remains limited. The aim of the study was to identify molecular genetic differences between C. quitensis populations in one of the Antarctic localities, the Argentine Islands region by estimating the intron length polymorphism of actin, α- and γ-tubulin genes. Samples of C. quitensis from different Antarctic natural populations were collected during the season of the 24th and previous Ukrainian Antarctic expeditions. Total DNA was isolated using the QIAGEN DNeasy Plant Mini Kit. The polymerase chain reaction was carried out with our own degenerate primers. The resulting amplicons were separated and visualized using polyacrylamide gel electrophoresis followed by silver nitrate staining. Molecular genetic analysis of natural populations of C. quitensis was performed using three DNA-marker systems based on the detection of intron length polymor-phism of actin, α- and γ-tubulin genes. A low level of genetic polymorphism of C. quitensis in the studied region by these types of markers was established. By assessing the intron length polymorphism of actin genes of the studied C. quitensis populations it was possible to establish that the populations of Skua Island had unique amplicons characteristic only for this location. This indicates the possibility of further use of the analysis of intron length polymorphism of actin genes for the study of the molecu-lar genetic diversity of the Antarctic pearlwort. At the same time, the results of analysis of the intron length polymorphism of α- and γ-tubulin genes induce selection of more specific primers, taking into account the structure of the C. quitensis genome. C. quitensis in the study region has a low level of genetic variability in intron length polymorphism of actin, α- and γ-tubulin genes. Overall, the results indicate that DNA markers based on gene structure analysis of highly conserved cytoskeletal pro-teins, namely, actin, α- and γ-tubulin, as additional sources of information, can be used for molecular genetic analysis of C. quitensis populations in the Antarctic. |
| first_indexed | 2024-03-11T13:10:36Z |
| format | Article |
| id | doaj.art-7016562860d047c49f2d80fef56456a8 |
| institution | Directory Open Access Journal |
| issn | 1727-7485 2415-3087 |
| language | English |
| last_indexed | 2024-03-11T13:10:36Z |
| publishDate | 2020-07-01 |
| publisher | State Institution National Antarctic Scientific Center |
| record_format | Article |
| series | Український антарктичний журнал |
| spelling | doaj.art-7016562860d047c49f2d80fef56456a82023-11-03T14:09:46ZengState Institution National Antarctic Scientific CenterУкраїнський антарктичний журнал1727-74852415-30872020-07-01193101https://doi.org/10.33275/1727-7485.1.2020.382382Assessment of Colobanthus quitensis genetic polymorphism from the Argentine Islands region (maritime Antarctic) by actin, α- and γ-tubulin gene intron analysisA. Rabokon0A. Postovoitovа1Yu. Bilonozhko2L. Kalafat3M. Pavlovska4Ie. Prekrasna5I. Parnikoza6I. Kozeretska7Ya. Pirko8Ya. Blume9Institute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, Kyiv, 04123, UkraineInstitute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, Kyiv, 04123, UkraineInstitute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, Kyiv, 04123, UkraineInstitute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, Kyiv, 04123, UkraineState Institution National Antarctic Scientific Center, Ministry of Education and Science of Ukraine, Kyiv, 01601, Ukraine; National University of Life and Environmental Sciences of Ukraine, Kyiv, 03041, Ukraine State Institution National Antarctic Scientific Center, Ministry of Education and Science of Ukraine, Kyiv, 01601, UkraineState Institution National Antarctic Scientific Center, Ministry of Education and Science of Ukraine, Kyiv, 01601, Ukraine; Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv, 03143, UkraineState Institution National Antarctic Scientific Center, Ministry of Education and Science of Ukraine, Kyiv, 01601, UkraineInstitute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, Kyiv, 04123, Ukraine Institute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, Kyiv, 04123, UkraineColobanthus quitensis is one of the two angiosperm plant species commonly spread in the Antarctic. The species has been extensively analyzed at morphological, anatomical and physiological levels, but information regarding its genetic vari-ability remains limited. The aim of the study was to identify molecular genetic differences between C. quitensis populations in one of the Antarctic localities, the Argentine Islands region by estimating the intron length polymorphism of actin, α- and γ-tubulin genes. Samples of C. quitensis from different Antarctic natural populations were collected during the season of the 24th and previous Ukrainian Antarctic expeditions. Total DNA was isolated using the QIAGEN DNeasy Plant Mini Kit. The polymerase chain reaction was carried out with our own degenerate primers. The resulting amplicons were separated and visualized using polyacrylamide gel electrophoresis followed by silver nitrate staining. Molecular genetic analysis of natural populations of C. quitensis was performed using three DNA-marker systems based on the detection of intron length polymor-phism of actin, α- and γ-tubulin genes. A low level of genetic polymorphism of C. quitensis in the studied region by these types of markers was established. By assessing the intron length polymorphism of actin genes of the studied C. quitensis populations it was possible to establish that the populations of Skua Island had unique amplicons characteristic only for this location. This indicates the possibility of further use of the analysis of intron length polymorphism of actin genes for the study of the molecu-lar genetic diversity of the Antarctic pearlwort. At the same time, the results of analysis of the intron length polymorphism of α- and γ-tubulin genes induce selection of more specific primers, taking into account the structure of the C. quitensis genome. C. quitensis in the study region has a low level of genetic variability in intron length polymorphism of actin, α- and γ-tubulin genes. Overall, the results indicate that DNA markers based on gene structure analysis of highly conserved cytoskeletal pro-teins, namely, actin, α- and γ-tubulin, as additional sources of information, can be used for molecular genetic analysis of C. quitensis populations in the Antarctic.http://uaj.uac.gov.ua/index.php/uaj/article/view/382colobanthus quitensismolecular genetic markersintron length polymorphismactinα-tubulinγ-tubulin |
| spellingShingle | A. Rabokon A. Postovoitovа Yu. Bilonozhko L. Kalafat M. Pavlovska Ie. Prekrasna I. Parnikoza I. Kozeretska Ya. Pirko Ya. Blume Assessment of Colobanthus quitensis genetic polymorphism from the Argentine Islands region (maritime Antarctic) by actin, α- and γ-tubulin gene intron analysis Український антарктичний журнал colobanthus quitensis molecular genetic markers intron length polymorphism actin α-tubulin γ-tubulin |
| title | Assessment of Colobanthus quitensis genetic polymorphism from the Argentine Islands region (maritime Antarctic) by actin, α- and γ-tubulin gene intron analysis |
| title_full | Assessment of Colobanthus quitensis genetic polymorphism from the Argentine Islands region (maritime Antarctic) by actin, α- and γ-tubulin gene intron analysis |
| title_fullStr | Assessment of Colobanthus quitensis genetic polymorphism from the Argentine Islands region (maritime Antarctic) by actin, α- and γ-tubulin gene intron analysis |
| title_full_unstemmed | Assessment of Colobanthus quitensis genetic polymorphism from the Argentine Islands region (maritime Antarctic) by actin, α- and γ-tubulin gene intron analysis |
| title_short | Assessment of Colobanthus quitensis genetic polymorphism from the Argentine Islands region (maritime Antarctic) by actin, α- and γ-tubulin gene intron analysis |
| title_sort | assessment of colobanthus quitensis genetic polymorphism from the argentine islands region maritime antarctic by actin α and γ tubulin gene intron analysis |
| topic | colobanthus quitensis molecular genetic markers intron length polymorphism actin α-tubulin γ-tubulin |
| url | http://uaj.uac.gov.ua/index.php/uaj/article/view/382 |
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