Sacubitril/valsartan inhibits the proliferation of vascular smooth muscle cells through notch signaling and ERK1/2 pathway
Abstract Aims To explore the role and mechanism of Notch signaling and ERK1/2 pathway in the inhibitory effect of sacubitril/valsartan on the proliferation of vascular smooth muscle cells (VSMCs). Main methods Human aortic vascular smooth muscle cells (HA-VSMCs) were cultured in vitro. The prolifera...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2024-02-01
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| Series: | BMC Cardiovascular Disorders |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12872-024-03764-8 |
| _version_ | 1797275989806415872 |
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| author | Congfeng Xu Ning Zhang Hong Yuan Liren Wang Yonghong Li |
| author_facet | Congfeng Xu Ning Zhang Hong Yuan Liren Wang Yonghong Li |
| author_sort | Congfeng Xu |
| collection | DOAJ |
| description | Abstract Aims To explore the role and mechanism of Notch signaling and ERK1/2 pathway in the inhibitory effect of sacubitril/valsartan on the proliferation of vascular smooth muscle cells (VSMCs). Main methods Human aortic vascular smooth muscle cells (HA-VSMCs) were cultured in vitro. The proliferating VSMCs were divided into three groups as control group, Ang II group and Ang II + sacubitril/valsartan group. Cell proliferation and migration were detected by CCK8 and scratch test respectively. The mRNA and protein expression of PCNA, MMP-9, Notch1 and Jagged-1 were detected by qRT-PCR and Western blot respectively. The p-ERK1/2 expression was detected by Western blot. Key findings Compared with the control group, proliferation and migration of VSMCs and the expression of PCNA, MMP-9, Notch1, Jagged-1 and p-ERK1/2 was increased in Ang II group. Sacubitril/valsartan significantly reduced the proliferation and migration. Additionally, pretreatment with sacubitril/valsartan reduced the PCNA, MMP-9, Notch1, Jagged-1 and p-ERK1/2 expression. |
| first_indexed | 2024-03-07T15:20:59Z |
| format | Article |
| id | doaj.art-701a6055b3604058bc2f89860cbf56b6 |
| institution | Directory Open Access Journal |
| issn | 1471-2261 |
| language | English |
| last_indexed | 2024-03-07T15:20:59Z |
| publishDate | 2024-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cardiovascular Disorders |
| spelling | doaj.art-701a6055b3604058bc2f89860cbf56b62024-03-05T17:37:31ZengBMCBMC Cardiovascular Disorders1471-22612024-02-0124111010.1186/s12872-024-03764-8Sacubitril/valsartan inhibits the proliferation of vascular smooth muscle cells through notch signaling and ERK1/2 pathwayCongfeng Xu0Ning Zhang1Hong Yuan2Liren Wang3Yonghong Li4Department of Cardiology, The Affiliated Hospital of Qingdao UniversityDepartment of Cardiology, The Affiliated Hospital of Qingdao UniversityDepartment of Cardiology, The Affiliated Hospital of Qingdao UniversityDepartment of Cardiology, The Affiliated Hospital of Qingdao UniversityDepartment of Cardiology, The Affiliated Hospital of Qingdao UniversityAbstract Aims To explore the role and mechanism of Notch signaling and ERK1/2 pathway in the inhibitory effect of sacubitril/valsartan on the proliferation of vascular smooth muscle cells (VSMCs). Main methods Human aortic vascular smooth muscle cells (HA-VSMCs) were cultured in vitro. The proliferating VSMCs were divided into three groups as control group, Ang II group and Ang II + sacubitril/valsartan group. Cell proliferation and migration were detected by CCK8 and scratch test respectively. The mRNA and protein expression of PCNA, MMP-9, Notch1 and Jagged-1 were detected by qRT-PCR and Western blot respectively. The p-ERK1/2 expression was detected by Western blot. Key findings Compared with the control group, proliferation and migration of VSMCs and the expression of PCNA, MMP-9, Notch1, Jagged-1 and p-ERK1/2 was increased in Ang II group. Sacubitril/valsartan significantly reduced the proliferation and migration. Additionally, pretreatment with sacubitril/valsartan reduced the PCNA, MMP-9, Notch1, Jagged-1 and p-ERK1/2 expression.https://doi.org/10.1186/s12872-024-03764-8RestenosisVascular smooth muscle cellsSacubitril/valsartanNotch1Jagged1ERK1/2 |
| spellingShingle | Congfeng Xu Ning Zhang Hong Yuan Liren Wang Yonghong Li Sacubitril/valsartan inhibits the proliferation of vascular smooth muscle cells through notch signaling and ERK1/2 pathway BMC Cardiovascular Disorders Restenosis Vascular smooth muscle cells Sacubitril/valsartan Notch1 Jagged1 ERK1/2 |
| title | Sacubitril/valsartan inhibits the proliferation of vascular smooth muscle cells through notch signaling and ERK1/2 pathway |
| title_full | Sacubitril/valsartan inhibits the proliferation of vascular smooth muscle cells through notch signaling and ERK1/2 pathway |
| title_fullStr | Sacubitril/valsartan inhibits the proliferation of vascular smooth muscle cells through notch signaling and ERK1/2 pathway |
| title_full_unstemmed | Sacubitril/valsartan inhibits the proliferation of vascular smooth muscle cells through notch signaling and ERK1/2 pathway |
| title_short | Sacubitril/valsartan inhibits the proliferation of vascular smooth muscle cells through notch signaling and ERK1/2 pathway |
| title_sort | sacubitril valsartan inhibits the proliferation of vascular smooth muscle cells through notch signaling and erk1 2 pathway |
| topic | Restenosis Vascular smooth muscle cells Sacubitril/valsartan Notch1 Jagged1 ERK1/2 |
| url | https://doi.org/10.1186/s12872-024-03764-8 |
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