Genome-Wide Identification of the Invertase Gene Family in Populus.
Invertase plays a crucial role in carbohydrate partitioning and plant development as it catalyses the irreversible hydrolysis of sucrose into glucose and fructose. The invertase family in plants is composed of two sub-families: acid invertases, which are targeted to the cell wall and vacuole; and ne...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4579127?pdf=render |
| _version_ | 1818996601050365952 |
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| author | Zhong Chen Kai Gao Xiaoxing Su Pian Rao Xinmin An |
| author_facet | Zhong Chen Kai Gao Xiaoxing Su Pian Rao Xinmin An |
| author_sort | Zhong Chen |
| collection | DOAJ |
| description | Invertase plays a crucial role in carbohydrate partitioning and plant development as it catalyses the irreversible hydrolysis of sucrose into glucose and fructose. The invertase family in plants is composed of two sub-families: acid invertases, which are targeted to the cell wall and vacuole; and neutral/alkaline invertases, which function in the cytosol. In this study, 5 cell wall invertase genes (PtCWINV1-5), 3 vacuolar invertase genes (PtVINV1-3) and 16 neutral/alkaline invertase genes (PtNINV1-16) were identified in the Populus genome and found to be distributed on 14 chromosomes. A comprehensive analysis of poplar invertase genes was performed, including structures, chromosome location, phylogeny, evolutionary pattern and expression profiles. Phylogenetic analysis indicated that the two sub-families were both divided into two clades. Segmental duplication is contributed to neutral/alkaline sub-family expansion. Furthermore, the Populus invertase genes displayed differential expression in roots, stems, leaves, leaf buds and in response to salt/cold stress and pathogen infection. In addition, the analysis of enzyme activity and sugar content revealed that invertase genes play key roles in the sucrose metabolism of various tissues and organs in poplar. This work lays the foundation for future functional analysis of the invertase genes in Populus and other woody perennials. |
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| format | Article |
| id | doaj.art-701a6cd6d10144fa822148fcaee2b732 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T21:32:20Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
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| spelling | doaj.art-701a6cd6d10144fa822148fcaee2b7322022-12-21T19:26:01ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01109e013854010.1371/journal.pone.0138540Genome-Wide Identification of the Invertase Gene Family in Populus.Zhong ChenKai GaoXiaoxing SuPian RaoXinmin AnInvertase plays a crucial role in carbohydrate partitioning and plant development as it catalyses the irreversible hydrolysis of sucrose into glucose and fructose. The invertase family in plants is composed of two sub-families: acid invertases, which are targeted to the cell wall and vacuole; and neutral/alkaline invertases, which function in the cytosol. In this study, 5 cell wall invertase genes (PtCWINV1-5), 3 vacuolar invertase genes (PtVINV1-3) and 16 neutral/alkaline invertase genes (PtNINV1-16) were identified in the Populus genome and found to be distributed on 14 chromosomes. A comprehensive analysis of poplar invertase genes was performed, including structures, chromosome location, phylogeny, evolutionary pattern and expression profiles. Phylogenetic analysis indicated that the two sub-families were both divided into two clades. Segmental duplication is contributed to neutral/alkaline sub-family expansion. Furthermore, the Populus invertase genes displayed differential expression in roots, stems, leaves, leaf buds and in response to salt/cold stress and pathogen infection. In addition, the analysis of enzyme activity and sugar content revealed that invertase genes play key roles in the sucrose metabolism of various tissues and organs in poplar. This work lays the foundation for future functional analysis of the invertase genes in Populus and other woody perennials.http://europepmc.org/articles/PMC4579127?pdf=render |
| spellingShingle | Zhong Chen Kai Gao Xiaoxing Su Pian Rao Xinmin An Genome-Wide Identification of the Invertase Gene Family in Populus. PLoS ONE |
| title | Genome-Wide Identification of the Invertase Gene Family in Populus. |
| title_full | Genome-Wide Identification of the Invertase Gene Family in Populus. |
| title_fullStr | Genome-Wide Identification of the Invertase Gene Family in Populus. |
| title_full_unstemmed | Genome-Wide Identification of the Invertase Gene Family in Populus. |
| title_short | Genome-Wide Identification of the Invertase Gene Family in Populus. |
| title_sort | genome wide identification of the invertase gene family in populus |
| url | http://europepmc.org/articles/PMC4579127?pdf=render |
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