Schinopsis brasiliensis Engl. to combat the biofilm-dependents diseases in vitro
Abstract Dental caries and periodontal disease are the most prevalent of the biofilm-dependent diseases. With numerous side effects on the use of chlorhexidine, the search for new safe therapeutic alternatives for microorganisms involved with these diseases increases every day. This study aimed to e...
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| Format: | Article |
| Language: | English |
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Academia Brasileira de Ciências
2020-11-01
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| Series: | Anais da Academia Brasileira de Ciências |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000700921&tlng=en |
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| author | PEDRO HENRIQUE SETTE-DE-SOUZA CLEILDO P. DE SANTANA ILZA MARIA O. SOUSA MARY ANN FOGLIO FRANCINALVA D. DE MEDEIROS ANA CLÁUDIA D. DE MEDEIROS |
| author_facet | PEDRO HENRIQUE SETTE-DE-SOUZA CLEILDO P. DE SANTANA ILZA MARIA O. SOUSA MARY ANN FOGLIO FRANCINALVA D. DE MEDEIROS ANA CLÁUDIA D. DE MEDEIROS |
| author_sort | PEDRO HENRIQUE SETTE-DE-SOUZA |
| collection | DOAJ |
| description | Abstract Dental caries and periodontal disease are the most prevalent of the biofilm-dependent diseases. With numerous side effects on the use of chlorhexidine, the search for new safe therapeutic alternatives for microorganisms involved with these diseases increases every day. This study aimed to evaluate the antimicrobial activity and cytotoxicity of extracts made from the bark of Schinopsis brasiliensis Engl. against five oral microorganisms and analyze their phytochemical and thermal degradation profile. The liquid-liquid partition was performed with hexane, chloroform and ethyl acetate. The identification and quantification of the chemical marker was done. Antimicrobial activity was evaluated based on the minimum inhibitory concentration. The cytotoxicity was analyzed based on the hemolysing potential of the samples. The thermal degradation profile was performed by two different methods. Gallic acid was identified as the main compound of the samples and showed the highest amount in the chloroform fraction. All samples were able to inhibit the growth of the microorganisms tested and showed no cytotoxicity. The ethanol extract absorbs less heat than the fractions. All samples exhibited exothermic peak consistent with degradation of gallic acid. Based on the results, the samples used are potential candidates for use in dental formulations for biofilm control. |
| first_indexed | 2024-04-11T17:26:49Z |
| format | Article |
| id | doaj.art-701da923ca0c4ee2a5ceabf2005c116f |
| institution | Directory Open Access Journal |
| issn | 1678-2690 |
| language | English |
| last_indexed | 2024-04-11T17:26:49Z |
| publishDate | 2020-11-01 |
| publisher | Academia Brasileira de Ciências |
| record_format | Article |
| series | Anais da Academia Brasileira de Ciências |
| spelling | doaj.art-701da923ca0c4ee2a5ceabf2005c116f2022-12-22T04:12:18ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902020-11-0192410.1590/0001-3765202020200408Schinopsis brasiliensis Engl. to combat the biofilm-dependents diseases in vitroPEDRO HENRIQUE SETTE-DE-SOUZAhttps://orcid.org/0000-0001-9119-8435CLEILDO P. DE SANTANAhttps://orcid.org/0000-0002-8097-7633ILZA MARIA O. SOUSAhttps://orcid.org/0000-0002-3956-3199MARY ANN FOGLIOhttps://orcid.org/0000-0001-7715-4452FRANCINALVA D. DE MEDEIROShttps://orcid.org/0000-0003-1812-6966ANA CLÁUDIA D. DE MEDEIROShttps://orcid.org/0000-0002-8682-0178Abstract Dental caries and periodontal disease are the most prevalent of the biofilm-dependent diseases. With numerous side effects on the use of chlorhexidine, the search for new safe therapeutic alternatives for microorganisms involved with these diseases increases every day. This study aimed to evaluate the antimicrobial activity and cytotoxicity of extracts made from the bark of Schinopsis brasiliensis Engl. against five oral microorganisms and analyze their phytochemical and thermal degradation profile. The liquid-liquid partition was performed with hexane, chloroform and ethyl acetate. The identification and quantification of the chemical marker was done. Antimicrobial activity was evaluated based on the minimum inhibitory concentration. The cytotoxicity was analyzed based on the hemolysing potential of the samples. The thermal degradation profile was performed by two different methods. Gallic acid was identified as the main compound of the samples and showed the highest amount in the chloroform fraction. All samples were able to inhibit the growth of the microorganisms tested and showed no cytotoxicity. The ethanol extract absorbs less heat than the fractions. All samples exhibited exothermic peak consistent with degradation of gallic acid. Based on the results, the samples used are potential candidates for use in dental formulations for biofilm control.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000700921&tlng=enantibacterial agentsnatural productsoral diseasesSchinopsis brasiliensis |
| spellingShingle | PEDRO HENRIQUE SETTE-DE-SOUZA CLEILDO P. DE SANTANA ILZA MARIA O. SOUSA MARY ANN FOGLIO FRANCINALVA D. DE MEDEIROS ANA CLÁUDIA D. DE MEDEIROS Schinopsis brasiliensis Engl. to combat the biofilm-dependents diseases in vitro Anais da Academia Brasileira de Ciências antibacterial agents natural products oral diseases Schinopsis brasiliensis |
| title | Schinopsis brasiliensis Engl. to combat the biofilm-dependents diseases in vitro |
| title_full | Schinopsis brasiliensis Engl. to combat the biofilm-dependents diseases in vitro |
| title_fullStr | Schinopsis brasiliensis Engl. to combat the biofilm-dependents diseases in vitro |
| title_full_unstemmed | Schinopsis brasiliensis Engl. to combat the biofilm-dependents diseases in vitro |
| title_short | Schinopsis brasiliensis Engl. to combat the biofilm-dependents diseases in vitro |
| title_sort | schinopsis brasiliensis engl to combat the biofilm dependents diseases in vitro |
| topic | antibacterial agents natural products oral diseases Schinopsis brasiliensis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000700921&tlng=en |
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