In vitro culture of mortiño (<em>Vaccinium floribundum</em> Kunth)
<p>Due to the economic and agronomic potencial that mortiño (<em>Vaccinium floribundum</em> Kunth) has in Ecuador, the objective of this research was to establish <em>in vitro</em> propagation methods for this species by seed germination and by shoot growth f...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Universidad San Francisco de Quito USFQ
2010-06-01
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| Series: | ACI Avances en Ciencias e Ingenierías |
| Subjects: | |
| Online Access: | http://revistas.usfq.edu.ec/index.php/avances/article/view/27 |
| Summary: | <p>Due to the economic and agronomic potencial that mortiño (<em>Vaccinium floribundum</em> Kunth) has in Ecuador, the objective of this research was to establish <em>in vitro</em> propagation methods for this species by seed germination and by shoot growth from axillary buds. Modified Woody Plant Medium [1] (mWPM) was used, in which up to 60.7% of seeds germinated. A strong cytokine, trans-zeatin riboside, TZR (1 mg/l), combined with a-napthaleneacetic acid, NAA (0.05 mg/l), was used to subculture the <em>in vitro</em> germinated plantlets. The use of basal medium without hormones or with 2iP, 6-(gamma,gamma-dimethylalylamino) pu­rine, allowed elongation and rooting of plantletss. The acclimatization of mortiño plants has not yet been standardized, and further research is needed to find the conditions that are suitable for this process. To establish <em>in vitro</em> cultures by axillary buds, apical segments of the stem were disinfected and introduced in medium mWPM containing TZR, (7 mg/l) combined with NAA (0.1 mg/l). Growing buds were transferred to media with 2iP (3 or 5 mg/l) for propagation and elongation. This investigation is a preliminary study for the <em>in vitro</em> culture of mortiño, setting the basis for future research with this species.</p> |
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| ISSN: | 1390-5384 2528-7788 |