Development of an acute pancreatitis porcine model based on endoscopic retrograde infusion of contrast medium or sodium taurocholate
Background/Aims A reproducible, endoscope-based, large animal model, of acute pancreatitis was developed to meet the need for a suitable means of preclinically testing treatments. The aim of this study was to develop an endoscope-based animal model of acute pancreatitis. Methods This experimental st...
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| Format: | Article |
| Language: | English |
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The Korean Association of Internal Medicine
2019-11-01
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| Series: | The Korean Journal of Internal Medicine |
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| Online Access: | http://www.kjim.org/upload/pdf/kjim-2017-367.pdf |
| _version_ | 1818607689681338368 |
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| author | Jin-Seok Park Seok Jeong Joon Mee Kim Bum Hei Lee Jae Min Kim Don Haeng Lee |
| author_facet | Jin-Seok Park Seok Jeong Joon Mee Kim Bum Hei Lee Jae Min Kim Don Haeng Lee |
| author_sort | Jin-Seok Park |
| collection | DOAJ |
| description | Background/Aims A reproducible, endoscope-based, large animal model, of acute pancreatitis was developed to meet the need for a suitable means of preclinically testing treatments. The aim of this study was to develop an endoscope-based animal model of acute pancreatitis. Methods This experimental study was conducted on six mini-pigs. The pancreatitis model was induced by infusing contrast medium (CM) or sodium taurocholate (TCA) under high pressure (100 mmHg) into the main pancreatic duct by endoscopic retrograde pancreatography. Animals were randomly allocated to three groups: a CM group, a 10% TCA group, and a 20% TCA group. Pancreatic injuries were evaluated histologically, and serum amylase and lipase levels were measured. Results Acute pancreatitis was observed in all animals during hematologic and histologic examinations. Serum amylase and lipase levels were significantly higher (> 10 times baseline), and pancreatic edema, vacuolization of acinar cells, and hemorrhagic necrosis were observed. Severity of pancreatitis tended to be greater in the TCA groups than in the CM group as assessed using histologic scores, and degrees of pancreatitis were found to be dose-dependently related to TCA concentration. Conclusions The two endoscopic procedures described are effective and safe for creating a swine model of acute pancreatitis. The authors hope the described endoscopic methods will assist in the development of a suitable treatment strategy. |
| first_indexed | 2024-12-16T14:30:45Z |
| format | Article |
| id | doaj.art-702719f4dd2b49149f6495678ba0573c |
| institution | Directory Open Access Journal |
| issn | 1226-3303 2005-6648 |
| language | English |
| last_indexed | 2024-12-16T14:30:45Z |
| publishDate | 2019-11-01 |
| publisher | The Korean Association of Internal Medicine |
| record_format | Article |
| series | The Korean Journal of Internal Medicine |
| spelling | doaj.art-702719f4dd2b49149f6495678ba0573c2022-12-21T22:28:14ZengThe Korean Association of Internal MedicineThe Korean Journal of Internal Medicine1226-33032005-66482019-11-013461244125110.3904/kjim.2017.367170140Development of an acute pancreatitis porcine model based on endoscopic retrograde infusion of contrast medium or sodium taurocholateJin-Seok Park0Seok Jeong1Joon Mee Kim2Bum Hei Lee3Jae Min Kim4Don Haeng Lee5 Digestive Disease Center, Department of Internal Medicine, Inha University School of Medicine, Incheon, Korea Digestive Disease Center, Department of Internal Medicine, Inha University School of Medicine, Incheon, Korea Department of Pathology, Inha University School of Medicine, Incheon, Korea The National Center of Efficacy Evaluation for the Development of Health Products Targeting Digestive Disorders (NCEED), Incheon, Korea The National Center of Efficacy Evaluation for the Development of Health Products Targeting Digestive Disorders (NCEED), Incheon, Korea Digestive Disease Center, Department of Internal Medicine, Inha University School of Medicine, Incheon, KoreaBackground/Aims A reproducible, endoscope-based, large animal model, of acute pancreatitis was developed to meet the need for a suitable means of preclinically testing treatments. The aim of this study was to develop an endoscope-based animal model of acute pancreatitis. Methods This experimental study was conducted on six mini-pigs. The pancreatitis model was induced by infusing contrast medium (CM) or sodium taurocholate (TCA) under high pressure (100 mmHg) into the main pancreatic duct by endoscopic retrograde pancreatography. Animals were randomly allocated to three groups: a CM group, a 10% TCA group, and a 20% TCA group. Pancreatic injuries were evaluated histologically, and serum amylase and lipase levels were measured. Results Acute pancreatitis was observed in all animals during hematologic and histologic examinations. Serum amylase and lipase levels were significantly higher (> 10 times baseline), and pancreatic edema, vacuolization of acinar cells, and hemorrhagic necrosis were observed. Severity of pancreatitis tended to be greater in the TCA groups than in the CM group as assessed using histologic scores, and degrees of pancreatitis were found to be dose-dependently related to TCA concentration. Conclusions The two endoscopic procedures described are effective and safe for creating a swine model of acute pancreatitis. The authors hope the described endoscopic methods will assist in the development of a suitable treatment strategy.http://www.kjim.org/upload/pdf/kjim-2017-367.pdfpancreatitisanimalsendoscopes |
| spellingShingle | Jin-Seok Park Seok Jeong Joon Mee Kim Bum Hei Lee Jae Min Kim Don Haeng Lee Development of an acute pancreatitis porcine model based on endoscopic retrograde infusion of contrast medium or sodium taurocholate The Korean Journal of Internal Medicine pancreatitis animals endoscopes |
| title | Development of an acute pancreatitis porcine model based on endoscopic retrograde infusion of contrast medium or sodium taurocholate |
| title_full | Development of an acute pancreatitis porcine model based on endoscopic retrograde infusion of contrast medium or sodium taurocholate |
| title_fullStr | Development of an acute pancreatitis porcine model based on endoscopic retrograde infusion of contrast medium or sodium taurocholate |
| title_full_unstemmed | Development of an acute pancreatitis porcine model based on endoscopic retrograde infusion of contrast medium or sodium taurocholate |
| title_short | Development of an acute pancreatitis porcine model based on endoscopic retrograde infusion of contrast medium or sodium taurocholate |
| title_sort | development of an acute pancreatitis porcine model based on endoscopic retrograde infusion of contrast medium or sodium taurocholate |
| topic | pancreatitis animals endoscopes |
| url | http://www.kjim.org/upload/pdf/kjim-2017-367.pdf |
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