Assessment of an exhaled breath test using ultraviolet photoionization time-of-flight mass spectrometry for the monitoring of kidney transplant recipients
Abstract Continuous monitoring for immunosuppressive status, infection and complications are a must for kidney transplantation (KTx) recipients. Traditional monitoring including blood sampling and kidney biopsy, which caused tremendous medical cost and trauma. Therefore, a cheaper and less invasive...
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| Format: | Article |
| Language: | English |
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Springer
2023-06-01
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| Series: | Molecular Biomedicine |
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| Online Access: | https://doi.org/10.1186/s43556-023-00130-6 |
| _version_ | 1797795915437703168 |
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| author | Shijian Feng Chengfang Xiang Yushi He Zhuoya Li Zhongjun Zhao Bohan Liu Zhaofa Yin Qiyu He Yanting Yang Zhongli Huang Tao Lin Wenwen Li Yixiang Duan |
| author_facet | Shijian Feng Chengfang Xiang Yushi He Zhuoya Li Zhongjun Zhao Bohan Liu Zhaofa Yin Qiyu He Yanting Yang Zhongli Huang Tao Lin Wenwen Li Yixiang Duan |
| author_sort | Shijian Feng |
| collection | DOAJ |
| description | Abstract Continuous monitoring for immunosuppressive status, infection and complications are a must for kidney transplantation (KTx) recipients. Traditional monitoring including blood sampling and kidney biopsy, which caused tremendous medical cost and trauma. Therefore, a cheaper and less invasive approach was urgently needed. We thought that a breath test has the potential to become a feasible tool for KTx monitoring. A prospective-specimen collection, retrospective-blinded assessment strategy was used in this study. Exhaled breath samples from 175 KTx recipients were collected in West China Hospital and tested by online ultraviolet photoionization time-of-flight mass spectrometry (UVP-TOF–MS). The classification models based on breath test performed well in classifying normal and abnormal values of creatinine, estimated glomerular filtration rate (eGFR), blood urea nitrogen (BUN) and tacrolimus, with AUC values of 0.889, 0.850, 0.849 and 0.889, respectively. Regression analysis also demonstrated the predictive ability of breath test for clinical creatinine, eGFR, BUN, tacrolimus level, as the predicted values obtained from the regression model correlated well with the clinical true values (p < 0.05). The findings of this investigation implied that a breath test by using UVP-TOF–MS for KTx recipient monitoring is possible and accurate, which might be useful for future clinical screenings. |
| first_indexed | 2024-03-13T03:25:14Z |
| format | Article |
| id | doaj.art-702c5831a9dc49658210066f1a1d7c19 |
| institution | Directory Open Access Journal |
| issn | 2662-8651 |
| language | English |
| last_indexed | 2024-03-13T03:25:14Z |
| publishDate | 2023-06-01 |
| publisher | Springer |
| record_format | Article |
| series | Molecular Biomedicine |
| spelling | doaj.art-702c5831a9dc49658210066f1a1d7c192023-06-25T11:03:55ZengSpringerMolecular Biomedicine2662-86512023-06-014111210.1186/s43556-023-00130-6Assessment of an exhaled breath test using ultraviolet photoionization time-of-flight mass spectrometry for the monitoring of kidney transplant recipientsShijian Feng0Chengfang Xiang1Yushi He2Zhuoya Li3Zhongjun Zhao4Bohan Liu5Zhaofa Yin6Qiyu He7Yanting Yang8Zhongli Huang9Tao Lin10Wenwen Li11Yixiang Duan12Department of Urology and Institute of Urology, Laboratory of Reconstructive Urology), West China Hospital, Sichuan UniversityCollege of Chemistry, Sichuan UniversityDepartment of Urology and Institute of Urology, Laboratory of Reconstructive Urology), West China Hospital, Sichuan UniversityWest China School of Public Health and West China Fourth Hospital, Sichuan UniversitySchool of Mechanical Engineering, Sichuan UniversityDepartment of Urology and Institute of Urology, Laboratory of Reconstructive Urology), West China Hospital, Sichuan UniversityDepartment of Urology and Institute of Urology, Laboratory of Reconstructive Urology), West China Hospital, Sichuan UniversityDepartment of Urology and Institute of Urology, Laboratory of Reconstructive Urology), West China Hospital, Sichuan UniversitySchool of Mechanical Engineering, Sichuan UniversityDepartment of Urology and Institute of Urology, Laboratory of Reconstructive Urology), West China Hospital, Sichuan UniversityDepartment of Urology and Institute of Urology, Laboratory of Reconstructive Urology), West China Hospital, Sichuan UniversityWest China School of Public Health and West China Fourth Hospital, Sichuan UniversitySchool of Mechanical Engineering, Sichuan UniversityAbstract Continuous monitoring for immunosuppressive status, infection and complications are a must for kidney transplantation (KTx) recipients. Traditional monitoring including blood sampling and kidney biopsy, which caused tremendous medical cost and trauma. Therefore, a cheaper and less invasive approach was urgently needed. We thought that a breath test has the potential to become a feasible tool for KTx monitoring. A prospective-specimen collection, retrospective-blinded assessment strategy was used in this study. Exhaled breath samples from 175 KTx recipients were collected in West China Hospital and tested by online ultraviolet photoionization time-of-flight mass spectrometry (UVP-TOF–MS). The classification models based on breath test performed well in classifying normal and abnormal values of creatinine, estimated glomerular filtration rate (eGFR), blood urea nitrogen (BUN) and tacrolimus, with AUC values of 0.889, 0.850, 0.849 and 0.889, respectively. Regression analysis also demonstrated the predictive ability of breath test for clinical creatinine, eGFR, BUN, tacrolimus level, as the predicted values obtained from the regression model correlated well with the clinical true values (p < 0.05). The findings of this investigation implied that a breath test by using UVP-TOF–MS for KTx recipient monitoring is possible and accurate, which might be useful for future clinical screenings.https://doi.org/10.1186/s43556-023-00130-6Kidney transplantationBreath testNoninvasive monitoring |
| spellingShingle | Shijian Feng Chengfang Xiang Yushi He Zhuoya Li Zhongjun Zhao Bohan Liu Zhaofa Yin Qiyu He Yanting Yang Zhongli Huang Tao Lin Wenwen Li Yixiang Duan Assessment of an exhaled breath test using ultraviolet photoionization time-of-flight mass spectrometry for the monitoring of kidney transplant recipients Molecular Biomedicine Kidney transplantation Breath test Noninvasive monitoring |
| title | Assessment of an exhaled breath test using ultraviolet photoionization time-of-flight mass spectrometry for the monitoring of kidney transplant recipients |
| title_full | Assessment of an exhaled breath test using ultraviolet photoionization time-of-flight mass spectrometry for the monitoring of kidney transplant recipients |
| title_fullStr | Assessment of an exhaled breath test using ultraviolet photoionization time-of-flight mass spectrometry for the monitoring of kidney transplant recipients |
| title_full_unstemmed | Assessment of an exhaled breath test using ultraviolet photoionization time-of-flight mass spectrometry for the monitoring of kidney transplant recipients |
| title_short | Assessment of an exhaled breath test using ultraviolet photoionization time-of-flight mass spectrometry for the monitoring of kidney transplant recipients |
| title_sort | assessment of an exhaled breath test using ultraviolet photoionization time of flight mass spectrometry for the monitoring of kidney transplant recipients |
| topic | Kidney transplantation Breath test Noninvasive monitoring |
| url | https://doi.org/10.1186/s43556-023-00130-6 |
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