Characterization of BoHV-1 gG-/tk-/gE- Mutant in Differential Protein Expression, Virulence, and Immunity
Infectious bovine rhinotracheitis (IBR), caused by bovine alphaherpesvirus 1 (BoHV-1), is an important disease affecting cattle worldwide resulting in great economic losses. Marker vaccines are effective in controlling infectious diseases including IBR, because they allow the discrimination between...
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MDPI AG
2021-10-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/8/11/253 |
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| author | Marawan A. Marawan Mingliang Deng Chen Wang Yingyu Chen Changmin Hu Jianguo Chen Xi Chen Huanchun Chen Aizhen Guo |
| author_facet | Marawan A. Marawan Mingliang Deng Chen Wang Yingyu Chen Changmin Hu Jianguo Chen Xi Chen Huanchun Chen Aizhen Guo |
| author_sort | Marawan A. Marawan |
| collection | DOAJ |
| description | Infectious bovine rhinotracheitis (IBR), caused by bovine alphaherpesvirus 1 (BoHV-1), is an important disease affecting cattle worldwide resulting in great economic losses. Marker vaccines are effective in controlling infectious diseases including IBR, because they allow the discrimination between the natural infection and the vaccination. Therefore, a triple gene deleted strain BoHV-1 gG-/tk-/gE- was developed and evaluated in vivo and in vitro as a marker vaccine. In cell culture, this triple mutant virus showed significantly slower growth kinetics and smaller plaques when compared to wild-type (wt) BoHV-1 and double mutant BoHV-1 gG-/tk- (<i>p</i> < 0.01). On proteomic level, it revealed downregulation of some virulence related proteins including thymidine kinase, glycoproteins G, E, I, and K when compared to the wt. In vitro, the triple mutant virus showed a significantly lower and shorter viral shedding period (<i>p</i> < 0.001) in calves compared to double mutant. Moreover, the immunized calves with triple mutant virus showed protection rates of 64.2% and 68.6% against wt BoHV-1 and wt BoHV-5 challenge, respectively, without reactivation of latency after dexamethasone injection. In conclusion, BoHV-1 gG-/tk-/gE- is a safer marker vaccine against IBR although its immunogenicity in calves was decreased when compared to double mutant virus. |
| first_indexed | 2024-03-10T04:59:11Z |
| format | Article |
| id | doaj.art-702d3ae7020849d78c92a325c4fe0e4a |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-10T04:59:11Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-702d3ae7020849d78c92a325c4fe0e4a2023-11-23T01:54:13ZengMDPI AGVeterinary Sciences2306-73812021-10-0181125310.3390/vetsci8110253Characterization of BoHV-1 gG-/tk-/gE- Mutant in Differential Protein Expression, Virulence, and ImmunityMarawan A. Marawan0Mingliang Deng1Chen Wang2Yingyu Chen3Changmin Hu4Jianguo Chen5Xi Chen6Huanchun Chen7Aizhen Guo8State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, ChinaInfectious bovine rhinotracheitis (IBR), caused by bovine alphaherpesvirus 1 (BoHV-1), is an important disease affecting cattle worldwide resulting in great economic losses. Marker vaccines are effective in controlling infectious diseases including IBR, because they allow the discrimination between the natural infection and the vaccination. Therefore, a triple gene deleted strain BoHV-1 gG-/tk-/gE- was developed and evaluated in vivo and in vitro as a marker vaccine. In cell culture, this triple mutant virus showed significantly slower growth kinetics and smaller plaques when compared to wild-type (wt) BoHV-1 and double mutant BoHV-1 gG-/tk- (<i>p</i> < 0.01). On proteomic level, it revealed downregulation of some virulence related proteins including thymidine kinase, glycoproteins G, E, I, and K when compared to the wt. In vitro, the triple mutant virus showed a significantly lower and shorter viral shedding period (<i>p</i> < 0.001) in calves compared to double mutant. Moreover, the immunized calves with triple mutant virus showed protection rates of 64.2% and 68.6% against wt BoHV-1 and wt BoHV-5 challenge, respectively, without reactivation of latency after dexamethasone injection. In conclusion, BoHV-1 gG-/tk-/gE- is a safer marker vaccine against IBR although its immunogenicity in calves was decreased when compared to double mutant virus.https://www.mdpi.com/2306-7381/8/11/253bovine herpesvirus 1,5proteomicsglycoproteinmarker vaccinesvirulenceimmunity |
| spellingShingle | Marawan A. Marawan Mingliang Deng Chen Wang Yingyu Chen Changmin Hu Jianguo Chen Xi Chen Huanchun Chen Aizhen Guo Characterization of BoHV-1 gG-/tk-/gE- Mutant in Differential Protein Expression, Virulence, and Immunity Veterinary Sciences bovine herpesvirus 1,5 proteomics glycoprotein marker vaccines virulence immunity |
| title | Characterization of BoHV-1 gG-/tk-/gE- Mutant in Differential Protein Expression, Virulence, and Immunity |
| title_full | Characterization of BoHV-1 gG-/tk-/gE- Mutant in Differential Protein Expression, Virulence, and Immunity |
| title_fullStr | Characterization of BoHV-1 gG-/tk-/gE- Mutant in Differential Protein Expression, Virulence, and Immunity |
| title_full_unstemmed | Characterization of BoHV-1 gG-/tk-/gE- Mutant in Differential Protein Expression, Virulence, and Immunity |
| title_short | Characterization of BoHV-1 gG-/tk-/gE- Mutant in Differential Protein Expression, Virulence, and Immunity |
| title_sort | characterization of bohv 1 gg tk ge mutant in differential protein expression virulence and immunity |
| topic | bovine herpesvirus 1,5 proteomics glycoprotein marker vaccines virulence immunity |
| url | https://www.mdpi.com/2306-7381/8/11/253 |
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