Detection of cytotoxic activity on Vero cells in clinical isolates of Serratia marcescens
Cytotoxin production was studied in 60 Serratia marcescens strains isolated from hospitalized patients. Association of cytotoxic activity with serotype, source of isolation and presence of plasmids was also evaluated. Thirteen of the 60 S. marcescens strains produced a cytotoxic effect on Vero cells...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Associação Brasileira de Divulgação Científica
1997-11-01
|
| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001100005 |
| _version_ | 1811202161810341888 |
|---|---|
| author | G.V. Carbonell A.F. Alfieri A.A. Alfieri M.C. Vidotto C.E. Levy A.L.C. Darini R.M. Yanaguita |
| author_facet | G.V. Carbonell A.F. Alfieri A.A. Alfieri M.C. Vidotto C.E. Levy A.L.C. Darini R.M. Yanaguita |
| author_sort | G.V. Carbonell |
| collection | DOAJ |
| description | Cytotoxin production was studied in 60 Serratia marcescens strains isolated from hospitalized patients. Association of cytotoxic activity with serotype, source of isolation and presence of plasmids was also evaluated. Thirteen of the 60 S. marcescens strains produced a cytotoxic effect on Vero cells. These strains were isolated from distinct clinical sources and classified into seven different serotypes (O1:H7; O4:NM; O10:NT; O19:NM; O6,14:H4; O6,14:NM and O6,14:H1). No relationship was observed between cytotoxic activity and clinical source or serotypes of the strains. Plasmids from five cytotoxin-producing S. marcescens strains were transferred to E. coli K12/711. The transconjugants did not exhibit cytotoxicity, indicating that the cytotoxic effect is not plasmid-mediated among these strains. Although a cytotoxic activity was demonstrated in filtrates of some S. marcescens strains, further studies should be performed to assess the role of this toxin in pathogenesis |
| first_indexed | 2024-04-12T02:35:37Z |
| format | Article |
| id | doaj.art-702ff9e460f44b0aa2167f2f2748fdc6 |
| institution | Directory Open Access Journal |
| issn | 0100-879X 1414-431X |
| language | English |
| last_indexed | 2024-04-12T02:35:37Z |
| publishDate | 1997-11-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-702ff9e460f44b0aa2167f2f2748fdc62022-12-22T03:51:36ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X1997-11-013011129110.1590/S0100-879X1997001100005Detection of cytotoxic activity on Vero cells in clinical isolates of Serratia marcescensG.V. CarbonellA.F. AlfieriA.A. AlfieriM.C. VidottoC.E. LevyA.L.C. DariniR.M. YanaguitaCytotoxin production was studied in 60 Serratia marcescens strains isolated from hospitalized patients. Association of cytotoxic activity with serotype, source of isolation and presence of plasmids was also evaluated. Thirteen of the 60 S. marcescens strains produced a cytotoxic effect on Vero cells. These strains were isolated from distinct clinical sources and classified into seven different serotypes (O1:H7; O4:NM; O10:NT; O19:NM; O6,14:H4; O6,14:NM and O6,14:H1). No relationship was observed between cytotoxic activity and clinical source or serotypes of the strains. Plasmids from five cytotoxin-producing S. marcescens strains were transferred to E. coli K12/711. The transconjugants did not exhibit cytotoxicity, indicating that the cytotoxic effect is not plasmid-mediated among these strains. Although a cytotoxic activity was demonstrated in filtrates of some S. marcescens strains, further studies should be performed to assess the role of this toxin in pathogenesishttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001100005cytotoxin productionnosocomial infectionsplasmidsSerratia marcescensvirulence factors |
| spellingShingle | G.V. Carbonell A.F. Alfieri A.A. Alfieri M.C. Vidotto C.E. Levy A.L.C. Darini R.M. Yanaguita Detection of cytotoxic activity on Vero cells in clinical isolates of Serratia marcescens Brazilian Journal of Medical and Biological Research cytotoxin production nosocomial infections plasmids Serratia marcescens virulence factors |
| title | Detection of cytotoxic activity on Vero cells in clinical isolates of Serratia marcescens |
| title_full | Detection of cytotoxic activity on Vero cells in clinical isolates of Serratia marcescens |
| title_fullStr | Detection of cytotoxic activity on Vero cells in clinical isolates of Serratia marcescens |
| title_full_unstemmed | Detection of cytotoxic activity on Vero cells in clinical isolates of Serratia marcescens |
| title_short | Detection of cytotoxic activity on Vero cells in clinical isolates of Serratia marcescens |
| title_sort | detection of cytotoxic activity on vero cells in clinical isolates of serratia marcescens |
| topic | cytotoxin production nosocomial infections plasmids Serratia marcescens virulence factors |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001100005 |
| work_keys_str_mv | AT gvcarbonell detectionofcytotoxicactivityonverocellsinclinicalisolatesofserratiamarcescens AT afalfieri detectionofcytotoxicactivityonverocellsinclinicalisolatesofserratiamarcescens AT aaalfieri detectionofcytotoxicactivityonverocellsinclinicalisolatesofserratiamarcescens AT mcvidotto detectionofcytotoxicactivityonverocellsinclinicalisolatesofserratiamarcescens AT celevy detectionofcytotoxicactivityonverocellsinclinicalisolatesofserratiamarcescens AT alcdarini detectionofcytotoxicactivityonverocellsinclinicalisolatesofserratiamarcescens AT rmyanaguita detectionofcytotoxicactivityonverocellsinclinicalisolatesofserratiamarcescens |