Nickel Nanoparticles Induce the Synthesis of a Tumor-Related Polypeptide in Human Epidermal Keratinocytes
Although nickel allergy and carcinogenicity are well known, their molecular mechanisms are still uncertain, thus demanding studies at the molecular level. The nickel carcinogenicity is known to be dependent on the chemical form of nickel, since only certain nickel compounds can enter the cell. This...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-05-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/10/5/992 |
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| author | Javier Jiménez-Lamana Simon Godin Gerard Aragonès Cinta Bladé Joanna Szpunar Ryszard Łobinski |
| author_facet | Javier Jiménez-Lamana Simon Godin Gerard Aragonès Cinta Bladé Joanna Szpunar Ryszard Łobinski |
| author_sort | Javier Jiménez-Lamana |
| collection | DOAJ |
| description | Although nickel allergy and carcinogenicity are well known, their molecular mechanisms are still uncertain, thus demanding studies at the molecular level. The nickel carcinogenicity is known to be dependent on the chemical form of nickel, since only certain nickel compounds can enter the cell. This study investigates, for the first time, the cytotoxicity, cellular uptake, and molecular targets of nickel nanoparticles (NiNPs) in human skin cells in comparison with other chemical forms of nickel. The dose-response curve that was obtained for NiNPs in the cytotoxicity assays showed a linear behavior typical of genotoxic carcinogens. The exposure of keratinocytes to NiNPs leads to the release of Ni<sup>2+</sup> ions and its accumulation in the cytosol. A 6 kDa nickel-binding molecule was found to be synthesized by cells exposed to NiNPs at a dose corresponding to medium mortality. This molecule was identified to be tumor-related p63-regulated gene 1 protein. |
| first_indexed | 2024-03-10T19:39:44Z |
| format | Article |
| id | doaj.art-7031b73e4e194acb84c71bb3e9ef2207 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T19:39:44Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-7031b73e4e194acb84c71bb3e9ef22072023-11-20T01:19:23ZengMDPI AGNanomaterials2079-49912020-05-0110599210.3390/nano10050992Nickel Nanoparticles Induce the Synthesis of a Tumor-Related Polypeptide in Human Epidermal KeratinocytesJavier Jiménez-Lamana0Simon Godin1Gerard Aragonès2Cinta Bladé3Joanna Szpunar4Ryszard Łobinski5Universite de Pau et des Pays de l’Adour, E2S UPPA, CNRS, IPREM UMR, 5254 Pau, FranceUniversite de Pau et des Pays de l’Adour, E2S UPPA, CNRS, IPREM UMR, 5254 Pau, FranceDepartment of Biochemistry and Biotechnology, Nutrigenomics Research Group, Universitat Rovira i Virgili, 43007 Tarragona, SpainDepartment of Biochemistry and Biotechnology, Nutrigenomics Research Group, Universitat Rovira i Virgili, 43007 Tarragona, SpainUniversite de Pau et des Pays de l’Adour, E2S UPPA, CNRS, IPREM UMR, 5254 Pau, FranceUniversite de Pau et des Pays de l’Adour, E2S UPPA, CNRS, IPREM UMR, 5254 Pau, FranceAlthough nickel allergy and carcinogenicity are well known, their molecular mechanisms are still uncertain, thus demanding studies at the molecular level. The nickel carcinogenicity is known to be dependent on the chemical form of nickel, since only certain nickel compounds can enter the cell. This study investigates, for the first time, the cytotoxicity, cellular uptake, and molecular targets of nickel nanoparticles (NiNPs) in human skin cells in comparison with other chemical forms of nickel. The dose-response curve that was obtained for NiNPs in the cytotoxicity assays showed a linear behavior typical of genotoxic carcinogens. The exposure of keratinocytes to NiNPs leads to the release of Ni<sup>2+</sup> ions and its accumulation in the cytosol. A 6 kDa nickel-binding molecule was found to be synthesized by cells exposed to NiNPs at a dose corresponding to medium mortality. This molecule was identified to be tumor-related p63-regulated gene 1 protein.https://www.mdpi.com/2079-4991/10/5/992nickel nanoparticleshuman keratinocytescytotoxicityprotein inductionhigh resolution mass spectrometry |
| spellingShingle | Javier Jiménez-Lamana Simon Godin Gerard Aragonès Cinta Bladé Joanna Szpunar Ryszard Łobinski Nickel Nanoparticles Induce the Synthesis of a Tumor-Related Polypeptide in Human Epidermal Keratinocytes Nanomaterials nickel nanoparticles human keratinocytes cytotoxicity protein induction high resolution mass spectrometry |
| title | Nickel Nanoparticles Induce the Synthesis of a Tumor-Related Polypeptide in Human Epidermal Keratinocytes |
| title_full | Nickel Nanoparticles Induce the Synthesis of a Tumor-Related Polypeptide in Human Epidermal Keratinocytes |
| title_fullStr | Nickel Nanoparticles Induce the Synthesis of a Tumor-Related Polypeptide in Human Epidermal Keratinocytes |
| title_full_unstemmed | Nickel Nanoparticles Induce the Synthesis of a Tumor-Related Polypeptide in Human Epidermal Keratinocytes |
| title_short | Nickel Nanoparticles Induce the Synthesis of a Tumor-Related Polypeptide in Human Epidermal Keratinocytes |
| title_sort | nickel nanoparticles induce the synthesis of a tumor related polypeptide in human epidermal keratinocytes |
| topic | nickel nanoparticles human keratinocytes cytotoxicity protein induction high resolution mass spectrometry |
| url | https://www.mdpi.com/2079-4991/10/5/992 |
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