Protective Effects of Fucoxanthin on High Glucose- and 4-Hydroxynonenal (4-HNE)-Induced Injury in Human Retinal Pigment Epithelial Cells
The incidence of diabetes mellitus is increasing due to the eating and living habits of modern people. As the disease progresses, the long-term effects of diabetes can cause microvascular disease, causing dysfunction in different parts of the body, which, in turn, leads to different complications, s...
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MDPI AG
2020-11-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/9/12/1176 |
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| author | Yi-Fen Chiang Hsin-Yuan Chen Yen-Jui Chang Yin-Hwa Shih Tzong-Ming Shieh Kai-Lee Wang Shih-Min Hsia |
| author_facet | Yi-Fen Chiang Hsin-Yuan Chen Yen-Jui Chang Yin-Hwa Shih Tzong-Ming Shieh Kai-Lee Wang Shih-Min Hsia |
| author_sort | Yi-Fen Chiang |
| collection | DOAJ |
| description | The incidence of diabetes mellitus is increasing due to the eating and living habits of modern people. As the disease progresses, the long-term effects of diabetes can cause microvascular disease, causing dysfunction in different parts of the body, which, in turn, leads to different complications, such as diabetic neuropathy, diabetic nephropathy, and diabetic retinopathy (DR). DR is the main cause of vision loss and blindness in diabetic patients. Persistent hyperglycemia may cause damage to the retina, induce the accumulation of inflammatory factors, and destroy the blood–retinal barrier function. Fucoxanthin (Fx) is a marine carotenoid extracted from seaweed. It accounts for more than 10% of the total carotenoids in nature. Fx is mainly found in brown algae and has strong antioxidant properties, due to its unique biologically active structure. This carotenoid also has the effects of reducing lipid peroxidation, reducing DNA damage, and preventing cardiovascular diseases as well as anti-inflammatory and anti-tumor effects. However, there is no relevant research on the protective effect of Fx in DR. Therefore, in this study, we explore the protective effect of Fx on the retina. Human retinal epithelial cells (ARPE-19) are used to investigate the protective effect of Fx on high glucose stress- (glucose 75 mM) and high lipid peroxidation stress (4-hydroxynonenal, 4-HNE (30 μM))-induced DR. The cell viability test shows that Fx recovered the cell damage, and Western blotting shows that Fx reduced the inflammation response and maintained the integrity of the blood–retinal barrier by reducing its apoptosis and cell adhesion factor protein expression. Using an antioxidant enzyme assay kit, we find that the protective effect of Fx may be related to the strong antioxidant properties of Fx, which increases catalase and reduces oxidative stress to produce a protective effect on the retina. |
| first_indexed | 2024-03-10T14:35:02Z |
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| id | doaj.art-7032eb60c70c40ea92b6fa45e7698e07 |
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| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T14:35:02Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-7032eb60c70c40ea92b6fa45e7698e072023-11-20T22:16:28ZengMDPI AGAntioxidants2076-39212020-11-01912117610.3390/antiox9121176Protective Effects of Fucoxanthin on High Glucose- and 4-Hydroxynonenal (4-HNE)-Induced Injury in Human Retinal Pigment Epithelial CellsYi-Fen Chiang0Hsin-Yuan Chen1Yen-Jui Chang2Yin-Hwa Shih3Tzong-Ming Shieh4Kai-Lee Wang5Shih-Min Hsia6School of Nutrition and Health Sciences, College of Nutrition, Taipei Medical University, Taipei 110301, TaiwanSchool of Nutrition and Health Sciences, College of Nutrition, Taipei Medical University, Taipei 110301, TaiwanDepartment of Ophthalmology, Yang Ming Branch, Taipei City Hospital, Taipei 111024, TaiwanDepartment of Healthcare Administration, Asia University, Taichung 41354, TaiwanSchool of Dentistry, College of Dentistry, China Medical University, Taichung 404333, TaiwanDepartment of Nursing, Ching Kuo Institute of Management and Health, Keelung City 203301, TaiwanSchool of Nutrition and Health Sciences, College of Nutrition, Taipei Medical University, Taipei 110301, TaiwanThe incidence of diabetes mellitus is increasing due to the eating and living habits of modern people. As the disease progresses, the long-term effects of diabetes can cause microvascular disease, causing dysfunction in different parts of the body, which, in turn, leads to different complications, such as diabetic neuropathy, diabetic nephropathy, and diabetic retinopathy (DR). DR is the main cause of vision loss and blindness in diabetic patients. Persistent hyperglycemia may cause damage to the retina, induce the accumulation of inflammatory factors, and destroy the blood–retinal barrier function. Fucoxanthin (Fx) is a marine carotenoid extracted from seaweed. It accounts for more than 10% of the total carotenoids in nature. Fx is mainly found in brown algae and has strong antioxidant properties, due to its unique biologically active structure. This carotenoid also has the effects of reducing lipid peroxidation, reducing DNA damage, and preventing cardiovascular diseases as well as anti-inflammatory and anti-tumor effects. However, there is no relevant research on the protective effect of Fx in DR. Therefore, in this study, we explore the protective effect of Fx on the retina. Human retinal epithelial cells (ARPE-19) are used to investigate the protective effect of Fx on high glucose stress- (glucose 75 mM) and high lipid peroxidation stress (4-hydroxynonenal, 4-HNE (30 μM))-induced DR. The cell viability test shows that Fx recovered the cell damage, and Western blotting shows that Fx reduced the inflammation response and maintained the integrity of the blood–retinal barrier by reducing its apoptosis and cell adhesion factor protein expression. Using an antioxidant enzyme assay kit, we find that the protective effect of Fx may be related to the strong antioxidant properties of Fx, which increases catalase and reduces oxidative stress to produce a protective effect on the retina.https://www.mdpi.com/2076-3921/9/12/1176fucoxanthinretinopathyantioxidant |
| spellingShingle | Yi-Fen Chiang Hsin-Yuan Chen Yen-Jui Chang Yin-Hwa Shih Tzong-Ming Shieh Kai-Lee Wang Shih-Min Hsia Protective Effects of Fucoxanthin on High Glucose- and 4-Hydroxynonenal (4-HNE)-Induced Injury in Human Retinal Pigment Epithelial Cells Antioxidants fucoxanthin retinopathy antioxidant |
| title | Protective Effects of Fucoxanthin on High Glucose- and 4-Hydroxynonenal (4-HNE)-Induced Injury in Human Retinal Pigment Epithelial Cells |
| title_full | Protective Effects of Fucoxanthin on High Glucose- and 4-Hydroxynonenal (4-HNE)-Induced Injury in Human Retinal Pigment Epithelial Cells |
| title_fullStr | Protective Effects of Fucoxanthin on High Glucose- and 4-Hydroxynonenal (4-HNE)-Induced Injury in Human Retinal Pigment Epithelial Cells |
| title_full_unstemmed | Protective Effects of Fucoxanthin on High Glucose- and 4-Hydroxynonenal (4-HNE)-Induced Injury in Human Retinal Pigment Epithelial Cells |
| title_short | Protective Effects of Fucoxanthin on High Glucose- and 4-Hydroxynonenal (4-HNE)-Induced Injury in Human Retinal Pigment Epithelial Cells |
| title_sort | protective effects of fucoxanthin on high glucose and 4 hydroxynonenal 4 hne induced injury in human retinal pigment epithelial cells |
| topic | fucoxanthin retinopathy antioxidant |
| url | https://www.mdpi.com/2076-3921/9/12/1176 |
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