Genetic Assessment of a Black Rockfish, Sebastes schlegelii, Stock Enhancement Program in Lidao Bay, China Based on Mitochondrial and Nuclear DNA Analysis
The black rockfish Sebastes schlegelii is a commercially important fish species for marine fishery stock enhancement in Asia. This work aimed to evaluate the potential genetic impacts of releasing hatchery-reared juvenile black rockfish on wild stock in Lidao Bay, China. A partial sequence of the mi...
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Frontiers Media S.A.
2020-02-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmars.2020.00094/full |
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| author | Lijuan Wang Lijuan Wang Zhihao Wu Zhihao Wu Yingjun Wang Mengxia Liu Aihuan Song Hongjun Liu Feng You Feng You |
| author_facet | Lijuan Wang Lijuan Wang Zhihao Wu Zhihao Wu Yingjun Wang Mengxia Liu Aihuan Song Hongjun Liu Feng You Feng You |
| author_sort | Lijuan Wang |
| collection | DOAJ |
| description | The black rockfish Sebastes schlegelii is a commercially important fish species for marine fishery stock enhancement in Asia. This work aimed to evaluate the potential genetic impacts of releasing hatchery-reared juvenile black rockfish on wild stock in Lidao Bay, China. A partial sequence of the mitochondrial DNA (mtDNA) control region and 38 microsatellite DNA loci were used to assess the genetic impact. The haplotype diversity parameter (h) and nucleotide diversity parameter (π) in hatchery-released stock were 0.902 and 0.00483, respectively. The h values in wild stock before stock enhancement and the mixed stock after enhancement were 0.970 and 0.939 (p = 0.025), respectively, and the π values were 0.00581 and 0.00526 (p = 0.150), respectively. The mean effective number of alleles (Ae) in hatchery-released stock was 4.76, the mean polymorphism information content (PIC) was 0.674, the observed heterozygosity (Ho) was 0.668, and the expected heterozygosity (He) was 0.697. In wild stock before stock enhancement and the mixed stock after enhancement, the Ae, PIC, Ho, and He values were 6.01 and 5.82, 0.698 and 0.716, 0.709 and 0.741, and 0.735 and 0.754, respectively. These results indicated no marked decrease in the hatchery-released stock, although it displayed slightly lower levels of genetic diversity and heterozygosity than the wild stock. And the mixed stock after release exhibited similar genetic diversity to that of the wild stock before release. Accordingly, we propose that stock enhancement may not cause genetic diversity reduction on wild S. schlegelii stock in Lidao Bay over the short term. Whereas, discriminant analysis of principal components (DAPC) analysis identified three major clusters. The fixation index (FST) and analysis of molecular variance (AMOVA) also clearly showed low level but significant differentiations between the hatchery-released and wild stocks, and the wild stock before enhancement and the mixed stock after enhancement. Consequently, long-term genetic evaluation might be required. |
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| language | English |
| last_indexed | 2024-12-14T02:12:16Z |
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| spelling | doaj.art-70352ee403284469ab2ec9baffba14e42022-12-21T23:20:43ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452020-02-01710.3389/fmars.2020.00094503181Genetic Assessment of a Black Rockfish, Sebastes schlegelii, Stock Enhancement Program in Lidao Bay, China Based on Mitochondrial and Nuclear DNA AnalysisLijuan Wang0Lijuan Wang1Zhihao Wu2Zhihao Wu3Yingjun Wang4Mengxia Liu5Aihuan Song6Hongjun Liu7Feng You8Feng You9Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaLaboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, ChinaKey Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaLaboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, ChinaMarine Biology Institute of Shandong Province, Qingdao, ChinaMarine Biology Institute of Shandong Province, Qingdao, ChinaMarine Biology Institute of Shandong Province, Qingdao, ChinaMarine Biology Institute of Shandong Province, Qingdao, ChinaKey Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaLaboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, ChinaThe black rockfish Sebastes schlegelii is a commercially important fish species for marine fishery stock enhancement in Asia. This work aimed to evaluate the potential genetic impacts of releasing hatchery-reared juvenile black rockfish on wild stock in Lidao Bay, China. A partial sequence of the mitochondrial DNA (mtDNA) control region and 38 microsatellite DNA loci were used to assess the genetic impact. The haplotype diversity parameter (h) and nucleotide diversity parameter (π) in hatchery-released stock were 0.902 and 0.00483, respectively. The h values in wild stock before stock enhancement and the mixed stock after enhancement were 0.970 and 0.939 (p = 0.025), respectively, and the π values were 0.00581 and 0.00526 (p = 0.150), respectively. The mean effective number of alleles (Ae) in hatchery-released stock was 4.76, the mean polymorphism information content (PIC) was 0.674, the observed heterozygosity (Ho) was 0.668, and the expected heterozygosity (He) was 0.697. In wild stock before stock enhancement and the mixed stock after enhancement, the Ae, PIC, Ho, and He values were 6.01 and 5.82, 0.698 and 0.716, 0.709 and 0.741, and 0.735 and 0.754, respectively. These results indicated no marked decrease in the hatchery-released stock, although it displayed slightly lower levels of genetic diversity and heterozygosity than the wild stock. And the mixed stock after release exhibited similar genetic diversity to that of the wild stock before release. Accordingly, we propose that stock enhancement may not cause genetic diversity reduction on wild S. schlegelii stock in Lidao Bay over the short term. Whereas, discriminant analysis of principal components (DAPC) analysis identified three major clusters. The fixation index (FST) and analysis of molecular variance (AMOVA) also clearly showed low level but significant differentiations between the hatchery-released and wild stocks, and the wild stock before enhancement and the mixed stock after enhancement. Consequently, long-term genetic evaluation might be required.https://www.frontiersin.org/article/10.3389/fmars.2020.00094/fullSebastes schlegeliimitochondrial DNAmicrosatellite DNAgenetic variationstock enhancementLidao Bay |
| spellingShingle | Lijuan Wang Lijuan Wang Zhihao Wu Zhihao Wu Yingjun Wang Mengxia Liu Aihuan Song Hongjun Liu Feng You Feng You Genetic Assessment of a Black Rockfish, Sebastes schlegelii, Stock Enhancement Program in Lidao Bay, China Based on Mitochondrial and Nuclear DNA Analysis Frontiers in Marine Science Sebastes schlegelii mitochondrial DNA microsatellite DNA genetic variation stock enhancement Lidao Bay |
| title | Genetic Assessment of a Black Rockfish, Sebastes schlegelii, Stock Enhancement Program in Lidao Bay, China Based on Mitochondrial and Nuclear DNA Analysis |
| title_full | Genetic Assessment of a Black Rockfish, Sebastes schlegelii, Stock Enhancement Program in Lidao Bay, China Based on Mitochondrial and Nuclear DNA Analysis |
| title_fullStr | Genetic Assessment of a Black Rockfish, Sebastes schlegelii, Stock Enhancement Program in Lidao Bay, China Based on Mitochondrial and Nuclear DNA Analysis |
| title_full_unstemmed | Genetic Assessment of a Black Rockfish, Sebastes schlegelii, Stock Enhancement Program in Lidao Bay, China Based on Mitochondrial and Nuclear DNA Analysis |
| title_short | Genetic Assessment of a Black Rockfish, Sebastes schlegelii, Stock Enhancement Program in Lidao Bay, China Based on Mitochondrial and Nuclear DNA Analysis |
| title_sort | genetic assessment of a black rockfish sebastes schlegelii stock enhancement program in lidao bay china based on mitochondrial and nuclear dna analysis |
| topic | Sebastes schlegelii mitochondrial DNA microsatellite DNA genetic variation stock enhancement Lidao Bay |
| url | https://www.frontiersin.org/article/10.3389/fmars.2020.00094/full |
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