The fungal community and its interaction with the concentration of short‐chain fatty acids in the faeces of Chenghua, Yorkshire and Tibetan pigs
Summary Despite their important roles in host nutrition and metabolism, and potential to cause disease, our knowledge of the fungal community in the mammalian gut is quite limited. To date, diversity and composition of fungi in swine gut still remains unknown. Therefore, the first internal transcrib...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-03-01
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| Series: | Microbial Biotechnology |
| Online Access: | https://doi.org/10.1111/1751-7915.13507 |
| _version_ | 1818204932344381440 |
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| author | Jiayan Li Daiwen Chen Bing Yu Jun He Zhiqing Huang Xiangbing Mao Ping Zheng Jie Yu Junqiu Luo Gang Tian Yuheng Luo |
| author_facet | Jiayan Li Daiwen Chen Bing Yu Jun He Zhiqing Huang Xiangbing Mao Ping Zheng Jie Yu Junqiu Luo Gang Tian Yuheng Luo |
| author_sort | Jiayan Li |
| collection | DOAJ |
| description | Summary Despite their important roles in host nutrition and metabolism, and potential to cause disease, our knowledge of the fungal community in the mammalian gut is quite limited. To date, diversity and composition of fungi in swine gut still remains unknown. Therefore, the first internal transcribed spacer of fungi in faecal samples from three breeds of pigs (10 pigs for each breed) was sequenced based on an Illumina HiSeq 2500 platform, and the relationship between the fungal community and the concentrations of main short‐chain fatty acids (SCFAs) was also analysed. Results indicated that Chenghua (local, higher body fat rate), Yorkshire (foreign, higher lean meat and growth rate) and Tibetan (plateau, stronger disease resistance) pigs harboured distinct fungal community. The Basidiomycota and Ascomycota presented as the two predominant phyla, with Loreleia, Russula and Candida as the top three genera in all samples. Network analysis revealed a total of 35 correlations among different fungal genera, with 27 (77.14%) positive and 8 (22.86%) negative pairwise interactions. Canonical correspondence analysis suggested that fungi in the faeces of pigs were more correlated to the concentration of acetate and butyrate rather than propionate. Spearman’s correlation further showed that Tomentella was positively correlated to both acetate and butyrate, and Loreleia was positively correlated to propionate (P < 0.05), while Nephroma and Taiwanofungus were negatively correlated to acetate and propionate (P < 0.05). These findings expanded our knowledge on the intestinal fungi in pigs with different genotypes and phenotypes, indicating that fungi may play an indispensable role during the metabolism of host and the maintenance of intestinal health. The cross‐feeding between fungi and other microorganisms may be crucial during the digestion of dietary carbohydrates and the associated physiological processes, which is worthy to be further studied. |
| first_indexed | 2024-12-12T03:49:05Z |
| format | Article |
| id | doaj.art-7037626c70a04da49570bc1a5799e874 |
| institution | Directory Open Access Journal |
| issn | 1751-7915 |
| language | English |
| last_indexed | 2024-12-12T03:49:05Z |
| publishDate | 2020-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Microbial Biotechnology |
| spelling | doaj.art-7037626c70a04da49570bc1a5799e8742022-12-22T00:39:27ZengWileyMicrobial Biotechnology1751-79152020-03-0113250952110.1111/1751-7915.13507The fungal community and its interaction with the concentration of short‐chain fatty acids in the faeces of Chenghua, Yorkshire and Tibetan pigsJiayan Li0Daiwen Chen1Bing Yu2Jun He3Zhiqing Huang4Xiangbing Mao5Ping Zheng6Jie Yu7Junqiu Luo8Gang Tian9Yuheng Luo10Key Laboratory for Animal Disease‐Resistance Nutrition Ministry of Education of China Chengdu ChinaKey Laboratory for Animal Disease‐Resistance Nutrition Ministry of Education of China Chengdu ChinaKey Laboratory for Animal Disease‐Resistance Nutrition Ministry of Education of China Chengdu ChinaKey Laboratory for Animal Disease‐Resistance Nutrition Ministry of Education of China Chengdu ChinaKey Laboratory for Animal Disease‐Resistance Nutrition Ministry of Education of China Chengdu ChinaKey Laboratory for Animal Disease‐Resistance Nutrition Ministry of Education of China Chengdu ChinaKey Laboratory for Animal Disease‐Resistance Nutrition Ministry of Education of China Chengdu ChinaKey Laboratory for Animal Disease‐Resistance Nutrition Ministry of Education of China Chengdu ChinaKey Laboratory for Animal Disease‐Resistance Nutrition Ministry of Education of China Chengdu ChinaKey Laboratory for Animal Disease‐Resistance Nutrition Ministry of Education of China Chengdu ChinaKey Laboratory for Animal Disease‐Resistance Nutrition Ministry of Education of China Chengdu ChinaSummary Despite their important roles in host nutrition and metabolism, and potential to cause disease, our knowledge of the fungal community in the mammalian gut is quite limited. To date, diversity and composition of fungi in swine gut still remains unknown. Therefore, the first internal transcribed spacer of fungi in faecal samples from three breeds of pigs (10 pigs for each breed) was sequenced based on an Illumina HiSeq 2500 platform, and the relationship between the fungal community and the concentrations of main short‐chain fatty acids (SCFAs) was also analysed. Results indicated that Chenghua (local, higher body fat rate), Yorkshire (foreign, higher lean meat and growth rate) and Tibetan (plateau, stronger disease resistance) pigs harboured distinct fungal community. The Basidiomycota and Ascomycota presented as the two predominant phyla, with Loreleia, Russula and Candida as the top three genera in all samples. Network analysis revealed a total of 35 correlations among different fungal genera, with 27 (77.14%) positive and 8 (22.86%) negative pairwise interactions. Canonical correspondence analysis suggested that fungi in the faeces of pigs were more correlated to the concentration of acetate and butyrate rather than propionate. Spearman’s correlation further showed that Tomentella was positively correlated to both acetate and butyrate, and Loreleia was positively correlated to propionate (P < 0.05), while Nephroma and Taiwanofungus were negatively correlated to acetate and propionate (P < 0.05). These findings expanded our knowledge on the intestinal fungi in pigs with different genotypes and phenotypes, indicating that fungi may play an indispensable role during the metabolism of host and the maintenance of intestinal health. The cross‐feeding between fungi and other microorganisms may be crucial during the digestion of dietary carbohydrates and the associated physiological processes, which is worthy to be further studied.https://doi.org/10.1111/1751-7915.13507 |
| spellingShingle | Jiayan Li Daiwen Chen Bing Yu Jun He Zhiqing Huang Xiangbing Mao Ping Zheng Jie Yu Junqiu Luo Gang Tian Yuheng Luo The fungal community and its interaction with the concentration of short‐chain fatty acids in the faeces of Chenghua, Yorkshire and Tibetan pigs Microbial Biotechnology |
| title | The fungal community and its interaction with the concentration of short‐chain fatty acids in the faeces of Chenghua, Yorkshire and Tibetan pigs |
| title_full | The fungal community and its interaction with the concentration of short‐chain fatty acids in the faeces of Chenghua, Yorkshire and Tibetan pigs |
| title_fullStr | The fungal community and its interaction with the concentration of short‐chain fatty acids in the faeces of Chenghua, Yorkshire and Tibetan pigs |
| title_full_unstemmed | The fungal community and its interaction with the concentration of short‐chain fatty acids in the faeces of Chenghua, Yorkshire and Tibetan pigs |
| title_short | The fungal community and its interaction with the concentration of short‐chain fatty acids in the faeces of Chenghua, Yorkshire and Tibetan pigs |
| title_sort | fungal community and its interaction with the concentration of short chain fatty acids in the faeces of chenghua yorkshire and tibetan pigs |
| url | https://doi.org/10.1111/1751-7915.13507 |
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