DETERMINATION OF VITAMIN C CONTENT IN BELL PEPPER (Capsicum annuum L.) WITH DIFFERENT PROTIC POLAR SOLVENT BY UV-VIS SPECTROSCOPY
Bell pepper can be an antioxidant and has many health benefits because of the high content of vitamin C. Vitamin C contents in yellow and orange bell peppers were extracted using different protic polar solvents and analyzed using the UV-Vis Spectrophotometry method. In this research, the bell pepper...
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| Format: | Article |
| Language: | English |
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Universitas Airlangga
2023-12-01
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| Series: | Jurnal Kimia Riset |
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| Online Access: | https://e-journal.unair.ac.id/JKR/article/view/44865 |
| _version_ | 1797349027995451392 |
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| author | Fawait Afnani Jamilah Hamidi Yanti Wiwit Sri Werdi Pratiwi |
| author_facet | Fawait Afnani Jamilah Hamidi Yanti Wiwit Sri Werdi Pratiwi |
| author_sort | Fawait Afnani |
| collection | DOAJ |
| description | Bell pepper can be an antioxidant and has many health benefits because of the high content of vitamin C. Vitamin C contents in yellow and orange bell peppers were extracted using different protic polar solvents and analyzed using the UV-Vis Spectrophotometry method. In this research, the bell peppers were extracted using the maceration technique for two days in 90% concentration of solvents (methanol and ethanol, respectively). Subsequently, the maximum wavelength was determined, and then 100 ppm ascorbic acid was used as a standard solution to analyze vitamin C content. Linearity based on a calibration curve is used to obtain the correlation coefficient of concentration between the standard solution and vitamin C levels in the sample. The result of UV-Vis spectroscopy analysis of this sample shows 373.5 nm of λmax. The linearity is shown in the equation y = 0.0006 x + 0.019. The vitamin C content in all samples had significant differences based on randomized complete block design (RCBD) test result with α = 0.05. The vitamin C level in the yellow pepper sample with an ethanol solvent (340 mg/100 g) was higher than that of the orange pepper sample with ethanol (251 mg/100 g). Meanwhile, the vitamin C content in the sample of yellow peppers with methanol solvent (562.5 mg/100 g) was smaller than that of orange peppers with methanol solvent (757.5 mg/100 g). These contents indicated a different result in the level sources of vitamin C, even if the maceration process used a solvent with a higher dielectric constant. |
| first_indexed | 2024-03-08T12:24:13Z |
| format | Article |
| id | doaj.art-7037e74c203540858803a1916843874e |
| institution | Directory Open Access Journal |
| issn | 2528-0414 2528-0422 |
| language | English |
| last_indexed | 2024-03-08T12:24:13Z |
| publishDate | 2023-12-01 |
| publisher | Universitas Airlangga |
| record_format | Article |
| series | Jurnal Kimia Riset |
| spelling | doaj.art-7037e74c203540858803a1916843874e2024-01-22T11:07:13ZengUniversitas AirlanggaJurnal Kimia Riset2528-04142528-04222023-12-018211612310.20473/jkr.v8i2.4486542950DETERMINATION OF VITAMIN C CONTENT IN BELL PEPPER (Capsicum annuum L.) WITH DIFFERENT PROTIC POLAR SOLVENT BY UV-VIS SPECTROSCOPYFawait Afnani0Jamilah Hamidi YantiWiwit Sri Werdi Pratiwi1Akademi Farmasi Yannas HusadaUniversity of Trunojoyo MaduraBell pepper can be an antioxidant and has many health benefits because of the high content of vitamin C. Vitamin C contents in yellow and orange bell peppers were extracted using different protic polar solvents and analyzed using the UV-Vis Spectrophotometry method. In this research, the bell peppers were extracted using the maceration technique for two days in 90% concentration of solvents (methanol and ethanol, respectively). Subsequently, the maximum wavelength was determined, and then 100 ppm ascorbic acid was used as a standard solution to analyze vitamin C content. Linearity based on a calibration curve is used to obtain the correlation coefficient of concentration between the standard solution and vitamin C levels in the sample. The result of UV-Vis spectroscopy analysis of this sample shows 373.5 nm of λmax. The linearity is shown in the equation y = 0.0006 x + 0.019. The vitamin C content in all samples had significant differences based on randomized complete block design (RCBD) test result with α = 0.05. The vitamin C level in the yellow pepper sample with an ethanol solvent (340 mg/100 g) was higher than that of the orange pepper sample with ethanol (251 mg/100 g). Meanwhile, the vitamin C content in the sample of yellow peppers with methanol solvent (562.5 mg/100 g) was smaller than that of orange peppers with methanol solvent (757.5 mg/100 g). These contents indicated a different result in the level sources of vitamin C, even if the maceration process used a solvent with a higher dielectric constant.https://e-journal.unair.ac.id/JKR/article/view/44865bell pepperprotic polar solventuv-vis spectroscopyvitamin c |
| spellingShingle | Fawait Afnani Jamilah Hamidi Yanti Wiwit Sri Werdi Pratiwi DETERMINATION OF VITAMIN C CONTENT IN BELL PEPPER (Capsicum annuum L.) WITH DIFFERENT PROTIC POLAR SOLVENT BY UV-VIS SPECTROSCOPY Jurnal Kimia Riset bell pepper protic polar solvent uv-vis spectroscopy vitamin c |
| title | DETERMINATION OF VITAMIN C CONTENT IN BELL PEPPER (Capsicum annuum L.) WITH DIFFERENT PROTIC POLAR SOLVENT BY UV-VIS SPECTROSCOPY |
| title_full | DETERMINATION OF VITAMIN C CONTENT IN BELL PEPPER (Capsicum annuum L.) WITH DIFFERENT PROTIC POLAR SOLVENT BY UV-VIS SPECTROSCOPY |
| title_fullStr | DETERMINATION OF VITAMIN C CONTENT IN BELL PEPPER (Capsicum annuum L.) WITH DIFFERENT PROTIC POLAR SOLVENT BY UV-VIS SPECTROSCOPY |
| title_full_unstemmed | DETERMINATION OF VITAMIN C CONTENT IN BELL PEPPER (Capsicum annuum L.) WITH DIFFERENT PROTIC POLAR SOLVENT BY UV-VIS SPECTROSCOPY |
| title_short | DETERMINATION OF VITAMIN C CONTENT IN BELL PEPPER (Capsicum annuum L.) WITH DIFFERENT PROTIC POLAR SOLVENT BY UV-VIS SPECTROSCOPY |
| title_sort | determination of vitamin c content in bell pepper capsicum annuum l with different protic polar solvent by uv vis spectroscopy |
| topic | bell pepper protic polar solvent uv-vis spectroscopy vitamin c |
| url | https://e-journal.unair.ac.id/JKR/article/view/44865 |
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