Serum protein coating enhances the antisepsis efficacy of silver nanoparticles against multidrug-resistant Escherichia coli infections in mice
Antimicrobial resistance poses a significant threat to public health and social development worldwide. This study aimed to investigate the effectiveness of silver nanoparticles (AgNPs) in treating multidrug-resistant bacterial infections. Eco-friendly spherical AgNPs were synthesized using rutin at...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1153147/full |
| _version_ | 1797821501333831680 |
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| author | Huamao Du Xiaoling Wang Hongying Zhang Heming Chen Xiaoyu Deng Yujing He Huaze Tang Fuchang Deng Zhihong Ren |
| author_facet | Huamao Du Xiaoling Wang Hongying Zhang Heming Chen Xiaoyu Deng Yujing He Huaze Tang Fuchang Deng Zhihong Ren |
| author_sort | Huamao Du |
| collection | DOAJ |
| description | Antimicrobial resistance poses a significant threat to public health and social development worldwide. This study aimed to investigate the effectiveness of silver nanoparticles (AgNPs) in treating multidrug-resistant bacterial infections. Eco-friendly spherical AgNPs were synthesized using rutin at room temperature. The biocompatibility of both polyvinyl pyrrolidone (PVP) and mouse serum (MS)-stabilized AgNPs was evaluated at 20 μg/mL and showed a similar distribution in mice. However, only MS-AgNPs significantly protected mice from sepsis caused by the multidrug-resistant Escherichia coli (E. coli) CQ10 strain (p = 0.039). The data revealed that MS-AgNPs facilitated the elimination of Escherichia coli (E. coli) in the blood and the spleen, and the mice experienced only a mild inflammatory response, as interleukin-6, tumor necrosis factor-α, chemokine KC, and C-reactive protein levels were significantly lower than those in the control group. The results suggest that the plasma protein corona strengthens the antibacterial effect of AgNPs in vivo and may be a potential strategy for combating antimicrobial resistance. |
| first_indexed | 2024-03-13T09:53:36Z |
| format | Article |
| id | doaj.art-7038db299d95487294719c6056a6555e |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-13T09:53:36Z |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-7038db299d95487294719c6056a6555e2023-05-24T05:03:51ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-05-011410.3389/fmicb.2023.11531471153147Serum protein coating enhances the antisepsis efficacy of silver nanoparticles against multidrug-resistant Escherichia coli infections in miceHuamao Du0Xiaoling Wang1Hongying Zhang2Heming Chen3Xiaoyu Deng4Yujing He5Huaze Tang6Fuchang Deng7Zhihong Ren8College of Biotechnology, Southwest University, Chongqing, ChinaClinical Laboratory, Shanxi Academy of Traditional Chinese Medicine, Shanxi Traditional Chinese Medicine Hospital, Taiyuan, ChinaCollege of Biotechnology, Southwest University, Chongqing, ChinaCollege of Biotechnology, Southwest University, Chongqing, ChinaCollege of Biotechnology, Southwest University, Chongqing, ChinaCollege of Biotechnology, Southwest University, Chongqing, ChinaCollege of Biotechnology, Southwest University, Chongqing, ChinaCollege of Biotechnology, Southwest University, Chongqing, ChinaChinese Center for Disease Control and Prevention, National Institute for Communicable Diseases Control and Prevention, Beijing, ChinaAntimicrobial resistance poses a significant threat to public health and social development worldwide. This study aimed to investigate the effectiveness of silver nanoparticles (AgNPs) in treating multidrug-resistant bacterial infections. Eco-friendly spherical AgNPs were synthesized using rutin at room temperature. The biocompatibility of both polyvinyl pyrrolidone (PVP) and mouse serum (MS)-stabilized AgNPs was evaluated at 20 μg/mL and showed a similar distribution in mice. However, only MS-AgNPs significantly protected mice from sepsis caused by the multidrug-resistant Escherichia coli (E. coli) CQ10 strain (p = 0.039). The data revealed that MS-AgNPs facilitated the elimination of Escherichia coli (E. coli) in the blood and the spleen, and the mice experienced only a mild inflammatory response, as interleukin-6, tumor necrosis factor-α, chemokine KC, and C-reactive protein levels were significantly lower than those in the control group. The results suggest that the plasma protein corona strengthens the antibacterial effect of AgNPs in vivo and may be a potential strategy for combating antimicrobial resistance.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1153147/fullprotein coronasilver nanoparticlesmultidrug-resistant bacteriasepsispro-inflammatory cytokine |
| spellingShingle | Huamao Du Xiaoling Wang Hongying Zhang Heming Chen Xiaoyu Deng Yujing He Huaze Tang Fuchang Deng Zhihong Ren Serum protein coating enhances the antisepsis efficacy of silver nanoparticles against multidrug-resistant Escherichia coli infections in mice Frontiers in Microbiology protein corona silver nanoparticles multidrug-resistant bacteria sepsis pro-inflammatory cytokine |
| title | Serum protein coating enhances the antisepsis efficacy of silver nanoparticles against multidrug-resistant Escherichia coli infections in mice |
| title_full | Serum protein coating enhances the antisepsis efficacy of silver nanoparticles against multidrug-resistant Escherichia coli infections in mice |
| title_fullStr | Serum protein coating enhances the antisepsis efficacy of silver nanoparticles against multidrug-resistant Escherichia coli infections in mice |
| title_full_unstemmed | Serum protein coating enhances the antisepsis efficacy of silver nanoparticles against multidrug-resistant Escherichia coli infections in mice |
| title_short | Serum protein coating enhances the antisepsis efficacy of silver nanoparticles against multidrug-resistant Escherichia coli infections in mice |
| title_sort | serum protein coating enhances the antisepsis efficacy of silver nanoparticles against multidrug resistant escherichia coli infections in mice |
| topic | protein corona silver nanoparticles multidrug-resistant bacteria sepsis pro-inflammatory cytokine |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1153147/full |
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