Relationship between red blood cell aggregation and dextran molecular mass
Abstract The aim of this study was to investigate the aggregation of red blood cells (RBCs) suspended in dextran solution at various levels of molecular mass. Dextran solutions at molecular mass 40, 70, 100 and 500 kDa at concentration from 2 to 5 g/dL were used to suspend the RBCs. The radius and v...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-11-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-24166-w |
| _version_ | 1811309252102324224 |
|---|---|
| author | Maciej Bosek Blanka Ziomkowska Jerzy Pyskir Tomasz Wybranowski Małgorzata Pyskir Michał Cyrankiewicz Marta Napiórkowska Maciej Durmowicz Stefan Kruszewski |
| author_facet | Maciej Bosek Blanka Ziomkowska Jerzy Pyskir Tomasz Wybranowski Małgorzata Pyskir Michał Cyrankiewicz Marta Napiórkowska Maciej Durmowicz Stefan Kruszewski |
| author_sort | Maciej Bosek |
| collection | DOAJ |
| description | Abstract The aim of this study was to investigate the aggregation of red blood cells (RBCs) suspended in dextran solution at various levels of molecular mass. Dextran solutions at molecular mass 40, 70, 100 and 500 kDa at concentration from 2 to 5 g/dL were used to suspend the RBCs. The radius and velocity of sedimenting RBC aggregates were investigated using image analysis. The radius and sedimentation velocity of aggregates increased initially, then decreased after achieving maxima. The maximal velocity of RBC aggregates showed a bell-shaped dependence on dextran molecular mass and concentration, whereas maximal radius showed monotonic increase with both factors. Difference between aggregate and solution density was estimated using aggregate radius and sedimentation velocity and dextran solution viscosity, and was consistent across most molecular mass and concentration levels. This allowed to calculate the porosity of aggregates and to show that it monotonically decreased with the increase in the solution density, caused by the increase in the dextran concentration. The results provide insight into the RBC aggregation process in solutions of proteins of different size, reflecting various pathological conditions. The currently reported data can be potentially applied to specific pathophysiological conditions giving an interpretation that is not yet fully discussed in the literature. |
| first_indexed | 2024-04-13T09:38:57Z |
| format | Article |
| id | doaj.art-703a1e8fff744350b0a1451a9c22ba66 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-13T09:38:57Z |
| publishDate | 2022-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-703a1e8fff744350b0a1451a9c22ba662022-12-22T02:52:00ZengNature PortfolioScientific Reports2045-23222022-11-0112111110.1038/s41598-022-24166-wRelationship between red blood cell aggregation and dextran molecular massMaciej Bosek0Blanka Ziomkowska1Jerzy Pyskir2Tomasz Wybranowski3Małgorzata Pyskir4Michał Cyrankiewicz5Marta Napiórkowska6Maciej Durmowicz7Stefan Kruszewski8Biophysics Department, Collegium Medicum of Nicolaus Copernicus UniversityBiophysics Department, Collegium Medicum of Nicolaus Copernicus UniversityBiophysics Department, Collegium Medicum of Nicolaus Copernicus UniversityBiophysics Department, Collegium Medicum of Nicolaus Copernicus UniversityDepartment of Rehabilitation, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus UniversityBiophysics Department, Collegium Medicum of Nicolaus Copernicus UniversityBiophysics Department, Collegium Medicum of Nicolaus Copernicus UniversityDepartment of Physiotherapy, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus UniversityBiophysics Department, Collegium Medicum of Nicolaus Copernicus UniversityAbstract The aim of this study was to investigate the aggregation of red blood cells (RBCs) suspended in dextran solution at various levels of molecular mass. Dextran solutions at molecular mass 40, 70, 100 and 500 kDa at concentration from 2 to 5 g/dL were used to suspend the RBCs. The radius and velocity of sedimenting RBC aggregates were investigated using image analysis. The radius and sedimentation velocity of aggregates increased initially, then decreased after achieving maxima. The maximal velocity of RBC aggregates showed a bell-shaped dependence on dextran molecular mass and concentration, whereas maximal radius showed monotonic increase with both factors. Difference between aggregate and solution density was estimated using aggregate radius and sedimentation velocity and dextran solution viscosity, and was consistent across most molecular mass and concentration levels. This allowed to calculate the porosity of aggregates and to show that it monotonically decreased with the increase in the solution density, caused by the increase in the dextran concentration. The results provide insight into the RBC aggregation process in solutions of proteins of different size, reflecting various pathological conditions. The currently reported data can be potentially applied to specific pathophysiological conditions giving an interpretation that is not yet fully discussed in the literature.https://doi.org/10.1038/s41598-022-24166-w |
| spellingShingle | Maciej Bosek Blanka Ziomkowska Jerzy Pyskir Tomasz Wybranowski Małgorzata Pyskir Michał Cyrankiewicz Marta Napiórkowska Maciej Durmowicz Stefan Kruszewski Relationship between red blood cell aggregation and dextran molecular mass Scientific Reports |
| title | Relationship between red blood cell aggregation and dextran molecular mass |
| title_full | Relationship between red blood cell aggregation and dextran molecular mass |
| title_fullStr | Relationship between red blood cell aggregation and dextran molecular mass |
| title_full_unstemmed | Relationship between red blood cell aggregation and dextran molecular mass |
| title_short | Relationship between red blood cell aggregation and dextran molecular mass |
| title_sort | relationship between red blood cell aggregation and dextran molecular mass |
| url | https://doi.org/10.1038/s41598-022-24166-w |
| work_keys_str_mv | AT maciejbosek relationshipbetweenredbloodcellaggregationanddextranmolecularmass AT blankaziomkowska relationshipbetweenredbloodcellaggregationanddextranmolecularmass AT jerzypyskir relationshipbetweenredbloodcellaggregationanddextranmolecularmass AT tomaszwybranowski relationshipbetweenredbloodcellaggregationanddextranmolecularmass AT małgorzatapyskir relationshipbetweenredbloodcellaggregationanddextranmolecularmass AT michałcyrankiewicz relationshipbetweenredbloodcellaggregationanddextranmolecularmass AT martanapiorkowska relationshipbetweenredbloodcellaggregationanddextranmolecularmass AT maciejdurmowicz relationshipbetweenredbloodcellaggregationanddextranmolecularmass AT stefankruszewski relationshipbetweenredbloodcellaggregationanddextranmolecularmass |