Reassessing the interpretation of oxidation–reduction potential in male infertility
Male Infertility Oxidative System (MiOXSYS) has been proposed as a rapid and promising technology for the evaluation of sperm oxidative stress. In this case–control study, 134 men with normal sperm parameters (NSP) and 574 men with abnormal sperm parameters (ASP), according to the World Health Organ...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Bioscientifica
2022-04-01
|
| Series: | Reproduction and Fertility |
| Subjects: | |
| Online Access: | https://raf.bioscientifica.com/view/journals/raf/3/2/RAF-21-0005.xml |
| _version_ | 1818351723833458688 |
|---|---|
| author | Fabien Joao Cyntia Duval Marie-Claire Bélanger Julie Lamoureux Cheng Wei Xiao Senem Ates Moncef Benkhalifa Pierre Miron |
| author_facet | Fabien Joao Cyntia Duval Marie-Claire Bélanger Julie Lamoureux Cheng Wei Xiao Senem Ates Moncef Benkhalifa Pierre Miron |
| author_sort | Fabien Joao |
| collection | DOAJ |
| description | Male Infertility Oxidative System (MiOXSYS) has been proposed as a rapid and promising technology for the evaluation of sperm oxidative stress. In this case–control study, 134 men with normal sperm parameters (NSP) and 574 men with abnormal sperm parameters (ASP), according to the World Health Organization sperm assessment references values established in 2010, were enrolled. Conventional sperm parameters were evaluated in all patients. Sperm static oxido-reduction potential (sORP) was assessed using the MiOXSYS. Sperm DNA integrity was measured in 604 patients. To ensure that sperm concentration was not a confounding factor in the sORP index ratio, sperm and seminal fluid sORP from 57 randomly selected additional patients were also measured using the MiOXSYS. sORP index (mV/106 sperm/ mL) was higher in patients with ASP and seemed to correlate with conventional sperm parameters. Although receiver-operating characteristic analysis revealed that a sORP index cut-off of 0.79 could differentiate normal from ASP with 57.7% sensitivity and 73.1% specificity, these values are much lower than those found in the literature. These values also need to be higher to be applicable in a clinical setting. Furthermore, absolute sORP (mV) was not different in the presence or absence of spermatozoa. sORP index relationships with sperm parameters seem rather be due to sperm concentration, denominator of the sORP index ratio. The establishment of a reliable method using the absolute sORP value, independent of sperm concentration, needs to be addressed. Other oxidative stress biomarkers could be used to validate this method. |
| first_indexed | 2024-12-13T18:42:17Z |
| format | Article |
| id | doaj.art-703a4020af2a45a1a0be8c16c0e11690 |
| institution | Directory Open Access Journal |
| issn | 2633-8386 |
| language | English |
| last_indexed | 2024-12-13T18:42:17Z |
| publishDate | 2022-04-01 |
| publisher | Bioscientifica |
| record_format | Article |
| series | Reproduction and Fertility |
| spelling | doaj.art-703a4020af2a45a1a0be8c16c0e116902022-12-21T23:35:10ZengBioscientificaReproduction and Fertility2633-83862022-04-01326776https://doi.org/10.1530/RAF-21-0005Reassessing the interpretation of oxidation–reduction potential in male infertilityFabien Joao0Cyntia Duval1Marie-Claire Bélanger2Julie Lamoureux3Cheng Wei Xiao4Senem Ates5Moncef Benkhalifa6Pierre Miron7Fertilys Fertility Center, Laval, Quebec, CanadaFertilys Fertility Center, Laval, Quebec, CanadaFertilys Fertility Center, Laval, Quebec, Canada; Centre de Recherche du Centre Hospitalier de l’Université de Montréal (CRCHUM), CHUM Research Center, Montreal, Quebec, CanadaFertilys Fertility Center, Laval, Quebec, CanadaFertilys Fertility Center, Laval, Quebec, CanadaFertilys Fertility Center, Laval, Quebec, CanadaFertilys Fertility Center, Laval, Quebec, Canada; Université Picardie Jules Verne, Médecine et Biologie de la Reproduction et Laboratoire PERITOX, CBH-CHU Amiens Picardie, Amiens, FranceFertilys Fertility Center, Laval, Quebec, CanadaMale Infertility Oxidative System (MiOXSYS) has been proposed as a rapid and promising technology for the evaluation of sperm oxidative stress. In this case–control study, 134 men with normal sperm parameters (NSP) and 574 men with abnormal sperm parameters (ASP), according to the World Health Organization sperm assessment references values established in 2010, were enrolled. Conventional sperm parameters were evaluated in all patients. Sperm static oxido-reduction potential (sORP) was assessed using the MiOXSYS. Sperm DNA integrity was measured in 604 patients. To ensure that sperm concentration was not a confounding factor in the sORP index ratio, sperm and seminal fluid sORP from 57 randomly selected additional patients were also measured using the MiOXSYS. sORP index (mV/106 sperm/ mL) was higher in patients with ASP and seemed to correlate with conventional sperm parameters. Although receiver-operating characteristic analysis revealed that a sORP index cut-off of 0.79 could differentiate normal from ASP with 57.7% sensitivity and 73.1% specificity, these values are much lower than those found in the literature. These values also need to be higher to be applicable in a clinical setting. Furthermore, absolute sORP (mV) was not different in the presence or absence of spermatozoa. sORP index relationships with sperm parameters seem rather be due to sperm concentration, denominator of the sORP index ratio. The establishment of a reliable method using the absolute sORP value, independent of sperm concentration, needs to be addressed. Other oxidative stress biomarkers could be used to validate this method.https://raf.bioscientifica.com/view/journals/raf/3/2/RAF-21-0005.xmlspermoxidoreduction potentialdna integritymale infertility |
| spellingShingle | Fabien Joao Cyntia Duval Marie-Claire Bélanger Julie Lamoureux Cheng Wei Xiao Senem Ates Moncef Benkhalifa Pierre Miron Reassessing the interpretation of oxidation–reduction potential in male infertility Reproduction and Fertility sperm oxidoreduction potential dna integrity male infertility |
| title | Reassessing the interpretation of oxidation–reduction potential in male infertility |
| title_full | Reassessing the interpretation of oxidation–reduction potential in male infertility |
| title_fullStr | Reassessing the interpretation of oxidation–reduction potential in male infertility |
| title_full_unstemmed | Reassessing the interpretation of oxidation–reduction potential in male infertility |
| title_short | Reassessing the interpretation of oxidation–reduction potential in male infertility |
| title_sort | reassessing the interpretation of oxidation reduction potential in male infertility |
| topic | sperm oxidoreduction potential dna integrity male infertility |
| url | https://raf.bioscientifica.com/view/journals/raf/3/2/RAF-21-0005.xml |
| work_keys_str_mv | AT fabienjoao reassessingtheinterpretationofoxidationreductionpotentialinmaleinfertility AT cyntiaduval reassessingtheinterpretationofoxidationreductionpotentialinmaleinfertility AT marieclairebelanger reassessingtheinterpretationofoxidationreductionpotentialinmaleinfertility AT julielamoureux reassessingtheinterpretationofoxidationreductionpotentialinmaleinfertility AT chengweixiao reassessingtheinterpretationofoxidationreductionpotentialinmaleinfertility AT senemates reassessingtheinterpretationofoxidationreductionpotentialinmaleinfertility AT moncefbenkhalifa reassessingtheinterpretationofoxidationreductionpotentialinmaleinfertility AT pierremiron reassessingtheinterpretationofoxidationreductionpotentialinmaleinfertility |