Epigallocatechin-3-Gallate (EGCG) Mitigates Endothelial and Circulating Cells Alterations Following PLLA Electrospun Mat Placement
Background. Synthetic vascular graft calcification is a serious complication of graft placement. Here, we analysed migration and osteogenic genes of human umbilical vein endothelial cells (HUVEC) cultured with a poly-L-lactic acid (PLLA) electrospun mat. The role of epigallo-catechin-3-gallate (EGCG...
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MDPI AG
2022-05-01
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| Online Access: | https://www.mdpi.com/2227-9059/10/6/1276 |
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| author | Carmen Ciavarella Ilenia Motta Santino Blando Sabrina Valente Fulvia Farabegoli Maria Letizia Focarete Mauro Gargiulo Gianandrea Pasquinelli |
| author_facet | Carmen Ciavarella Ilenia Motta Santino Blando Sabrina Valente Fulvia Farabegoli Maria Letizia Focarete Mauro Gargiulo Gianandrea Pasquinelli |
| author_sort | Carmen Ciavarella |
| collection | DOAJ |
| description | Background. Synthetic vascular graft calcification is a serious complication of graft placement. Here, we analysed migration and osteogenic genes of human umbilical vein endothelial cells (HUVEC) cultured with a poly-L-lactic acid (PLLA) electrospun mat. The role of epigallo-catechin-3-gallate (EGCG) in pathogenic processes involving HUVEC and peripheral blood mononuclear cells (PBMCs) was also tested. Methods. HUVEC were cultured in indirect contact with PLLA for 48 h, with or without EGCG, and processed for mRNA expression. HUVEC proliferation, migration and osteogenic differentiation were evaluated after EGCG treatment. EGCG was also administrated to human PBMCs, to analyse proliferation and migration toward HUVEC cultured with PLLA. Results. HUVEC cultured with PLLA exhibited increased expression of SLUG, VIMENTIN, MMP-9 (migration, vascular remodelling) and RUNX-2 (osteogenic transcription factor). EGCG at 25 μM significantly reduced HUVEC migration, osteogenic differentiation, without affecting cell viability, and mitigated PLLA influence on SLUG, MMP-9, VIMENTIN and RUNX-2 expression. EGCG affected PBMC proliferation and migration toward PLLA in a transwell co-culture system with HUVEC. Conclusion. Our study suggests the pro-calcific effect of PLLA, proposing EGCG as an anti-inflammatory modulatory approach. Research efforts need to deepen PLLA-vascular wall interactions for preventing vascular graft failure. |
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| format | Article |
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| language | English |
| last_indexed | 2024-03-10T00:21:52Z |
| publishDate | 2022-05-01 |
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| series | Biomedicines |
| spelling | doaj.art-703b3a36340d4fdca6606300486a21152023-11-23T15:41:58ZengMDPI AGBiomedicines2227-90592022-05-01106127610.3390/biomedicines10061276Epigallocatechin-3-Gallate (EGCG) Mitigates Endothelial and Circulating Cells Alterations Following PLLA Electrospun Mat PlacementCarmen Ciavarella0Ilenia Motta1Santino Blando2Sabrina Valente3Fulvia Farabegoli4Maria Letizia Focarete5Mauro Gargiulo6Gianandrea Pasquinelli7Department of Experimental, Diagnostic and Specialty Medicine (DIMES), University of Bologna, 40138 Bologna, ItalyDepartment of Experimental, Diagnostic and Specialty Medicine (DIMES), University of Bologna, 40138 Bologna, ItalyDepartment of Experimental, Diagnostic and Specialty Medicine (DIMES), University of Bologna, 40138 Bologna, ItalyDepartment of Experimental, Diagnostic and Specialty Medicine (DIMES), University of Bologna, 40138 Bologna, ItalyFABIT—Department of Pharmacy and Biotechnology, University of Bologna, 40126 Bologna, ItalyDepartment of Chemistry “Giacomo Ciamician”, University of Bologna, 40126 Bologna, ItalyDepartment of Experimental, Diagnostic and Specialty Medicine (DIMES), University of Bologna, 40138 Bologna, ItalyDepartment of Experimental, Diagnostic and Specialty Medicine (DIMES), University of Bologna, 40138 Bologna, ItalyBackground. Synthetic vascular graft calcification is a serious complication of graft placement. Here, we analysed migration and osteogenic genes of human umbilical vein endothelial cells (HUVEC) cultured with a poly-L-lactic acid (PLLA) electrospun mat. The role of epigallo-catechin-3-gallate (EGCG) in pathogenic processes involving HUVEC and peripheral blood mononuclear cells (PBMCs) was also tested. Methods. HUVEC were cultured in indirect contact with PLLA for 48 h, with or without EGCG, and processed for mRNA expression. HUVEC proliferation, migration and osteogenic differentiation were evaluated after EGCG treatment. EGCG was also administrated to human PBMCs, to analyse proliferation and migration toward HUVEC cultured with PLLA. Results. HUVEC cultured with PLLA exhibited increased expression of SLUG, VIMENTIN, MMP-9 (migration, vascular remodelling) and RUNX-2 (osteogenic transcription factor). EGCG at 25 μM significantly reduced HUVEC migration, osteogenic differentiation, without affecting cell viability, and mitigated PLLA influence on SLUG, MMP-9, VIMENTIN and RUNX-2 expression. EGCG affected PBMC proliferation and migration toward PLLA in a transwell co-culture system with HUVEC. Conclusion. Our study suggests the pro-calcific effect of PLLA, proposing EGCG as an anti-inflammatory modulatory approach. Research efforts need to deepen PLLA-vascular wall interactions for preventing vascular graft failure.https://www.mdpi.com/2227-9059/10/6/1276poly-L-lactic acid (PLLA)vascular graftcalcificationinflammationepigallo-catechin-3-gallate (EGCG) |
| spellingShingle | Carmen Ciavarella Ilenia Motta Santino Blando Sabrina Valente Fulvia Farabegoli Maria Letizia Focarete Mauro Gargiulo Gianandrea Pasquinelli Epigallocatechin-3-Gallate (EGCG) Mitigates Endothelial and Circulating Cells Alterations Following PLLA Electrospun Mat Placement Biomedicines poly-L-lactic acid (PLLA) vascular graft calcification inflammation epigallo-catechin-3-gallate (EGCG) |
| title | Epigallocatechin-3-Gallate (EGCG) Mitigates Endothelial and Circulating Cells Alterations Following PLLA Electrospun Mat Placement |
| title_full | Epigallocatechin-3-Gallate (EGCG) Mitigates Endothelial and Circulating Cells Alterations Following PLLA Electrospun Mat Placement |
| title_fullStr | Epigallocatechin-3-Gallate (EGCG) Mitigates Endothelial and Circulating Cells Alterations Following PLLA Electrospun Mat Placement |
| title_full_unstemmed | Epigallocatechin-3-Gallate (EGCG) Mitigates Endothelial and Circulating Cells Alterations Following PLLA Electrospun Mat Placement |
| title_short | Epigallocatechin-3-Gallate (EGCG) Mitigates Endothelial and Circulating Cells Alterations Following PLLA Electrospun Mat Placement |
| title_sort | epigallocatechin 3 gallate egcg mitigates endothelial and circulating cells alterations following plla electrospun mat placement |
| topic | poly-L-lactic acid (PLLA) vascular graft calcification inflammation epigallo-catechin-3-gallate (EGCG) |
| url | https://www.mdpi.com/2227-9059/10/6/1276 |
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