In vitro effect of Acanthospermum australe (Asteraceae) extracts on Acanthamoeba polyphaga trophozoites
Acanthamoeba is a free-living protozoan widely distributed in the environment, occurring in vegetative trophozoite and resistance cyst stages during its life cycle. It constitutes an etiological agent of Acanthamoeba keratitis, a disease that may cause severe ocular inflammation and blindness. New d...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Universidade Estadual Paulista
2013-01-01
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| Series: | Revista Brasileira de Plantas Medicinais |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-05722013000400016&lng=en&tlng=en |
| _version_ | 1819037149292396544 |
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| author | LC Castro IP Sauter EM Ethur C Kauffmann R Dall'agnol J Souza SP Cibulski AW Muniz L Weidlich PM Lohmann PM Roehe JC Germani MB Rott ST Vand Der Sand |
| author_facet | LC Castro IP Sauter EM Ethur C Kauffmann R Dall'agnol J Souza SP Cibulski AW Muniz L Weidlich PM Lohmann PM Roehe JC Germani MB Rott ST Vand Der Sand |
| author_sort | LC Castro |
| collection | DOAJ |
| description | Acanthamoeba is a free-living protozoan widely distributed in the environment, occurring in vegetative trophozoite and resistance cyst stages during its life cycle. It constitutes an etiological agent of Acanthamoeba keratitis, a disease that may cause severe ocular inflammation and blindness. New drugs can be developed from molecules found in plants and thus help in its difficult treatment. Acanthospermum australe (Asteraceae), a plant used in folk medicine, had its effect tested on Acanthamoeba polyphaga. Aqueous and ethanolic extracts of A. austral were obtained from aerial parts for infusion and static maceration, respectively. Concentrations of 10, 5, 2.5, 1.25 and 0.625 mg/ml of the extract were tested against Acanthamoeba polyphaga trophozoites. The cytotoxic effect of the extracts was tested in mammalian cells using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. RESULTS: The 10 mg/ml concentration of ethanolic extract was lethal to 100% of the A. polyphaga trophozoites in 24 h and both extracts presented cytotoxic effect against mammalian cells. These findings suggest that the A. austral ethanolic extract may have compounds with relevance to the development of new amoebicidal drugs. |
| first_indexed | 2024-12-21T08:16:49Z |
| format | Article |
| id | doaj.art-703e0881f0404d87a9f888f44d3820d9 |
| institution | Directory Open Access Journal |
| issn | 1983-084X |
| language | English |
| last_indexed | 2024-12-21T08:16:49Z |
| publishDate | 2013-01-01 |
| publisher | Universidade Estadual Paulista |
| record_format | Article |
| series | Revista Brasileira de Plantas Medicinais |
| spelling | doaj.art-703e0881f0404d87a9f888f44d3820d92022-12-21T19:10:32ZengUniversidade Estadual PaulistaRevista Brasileira de Plantas Medicinais1983-084X2013-01-0115458959410.1590/S1516-05722013000400016S1516-05722013000400016In vitro effect of Acanthospermum australe (Asteraceae) extracts on Acanthamoeba polyphaga trophozoitesLC Castro0IP Sauter1EM Ethur2C Kauffmann3R Dall'agnol4J Souza5SP Cibulski6AW Muniz7L Weidlich8PM Lohmann9PM Roehe10JC Germani11MB Rott12ST Vand Der Sand13Universidade Federal do Rio Grande do SulUniversidade Federal do Rio Grande do SulCentro Universitário UnivatesCentro Universitário UnivatesCentro Universitário UnivatesCentro Universitário UnivatesUniversidade Federal do Rio Grande do SulCentro Universitário UnivatesCentro Universitário UnivatesCentro Universitário UnivatesUniversidade Federal do Rio Grande do SulUniversidade Federal do Rio Grande do SulUniversidade Federal do Rio Grande do SulUniversidade Federal do Rio Grande do SulAcanthamoeba is a free-living protozoan widely distributed in the environment, occurring in vegetative trophozoite and resistance cyst stages during its life cycle. It constitutes an etiological agent of Acanthamoeba keratitis, a disease that may cause severe ocular inflammation and blindness. New drugs can be developed from molecules found in plants and thus help in its difficult treatment. Acanthospermum australe (Asteraceae), a plant used in folk medicine, had its effect tested on Acanthamoeba polyphaga. Aqueous and ethanolic extracts of A. austral were obtained from aerial parts for infusion and static maceration, respectively. Concentrations of 10, 5, 2.5, 1.25 and 0.625 mg/ml of the extract were tested against Acanthamoeba polyphaga trophozoites. The cytotoxic effect of the extracts was tested in mammalian cells using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. RESULTS: The 10 mg/ml concentration of ethanolic extract was lethal to 100% of the A. polyphaga trophozoites in 24 h and both extracts presented cytotoxic effect against mammalian cells. These findings suggest that the A. austral ethanolic extract may have compounds with relevance to the development of new amoebicidal drugs.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-05722013000400016&lng=en&tlng=enAcanthamoeba polyphagaatividade amebicidaAcanthospermum australe |
| spellingShingle | LC Castro IP Sauter EM Ethur C Kauffmann R Dall'agnol J Souza SP Cibulski AW Muniz L Weidlich PM Lohmann PM Roehe JC Germani MB Rott ST Vand Der Sand In vitro effect of Acanthospermum australe (Asteraceae) extracts on Acanthamoeba polyphaga trophozoites Revista Brasileira de Plantas Medicinais Acanthamoeba polyphaga atividade amebicida Acanthospermum australe |
| title | In vitro effect of Acanthospermum australe (Asteraceae) extracts on Acanthamoeba polyphaga trophozoites |
| title_full | In vitro effect of Acanthospermum australe (Asteraceae) extracts on Acanthamoeba polyphaga trophozoites |
| title_fullStr | In vitro effect of Acanthospermum australe (Asteraceae) extracts on Acanthamoeba polyphaga trophozoites |
| title_full_unstemmed | In vitro effect of Acanthospermum australe (Asteraceae) extracts on Acanthamoeba polyphaga trophozoites |
| title_short | In vitro effect of Acanthospermum australe (Asteraceae) extracts on Acanthamoeba polyphaga trophozoites |
| title_sort | in vitro effect of acanthospermum australe asteraceae extracts on acanthamoeba polyphaga trophozoites |
| topic | Acanthamoeba polyphaga atividade amebicida Acanthospermum australe |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-05722013000400016&lng=en&tlng=en |
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