Overlooked KCNQ4 variants augment the risk of hearing loss
Abstract Pathogenic variants of KCNQ4 cause symmetrical, late-onset, progressive, high-frequency-affected hearing loss, which eventually involves all frequencies with age. To understand the contribution of KCNQ4 variants to hearing loss, we analyzed whole-exome and genome sequencing data from patien...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2023-04-01
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| Series: | Experimental and Molecular Medicine |
| Online Access: | https://doi.org/10.1038/s12276-023-00976-4 |
| _version_ | 1797827644991995904 |
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| author | Kyung Seok Oh Jae Won Roh Sun Young Joo Kunhi Ryu Jung Ah Kim Se Jin Kim Seung Hyun Jang Young Ik Koh Da Hye Kim Hye-Youn Kim Murim Choi Jinsei Jung Wan Namkung Joo Hyun Nam Jae Young Choi Heon Yung Gee |
| author_facet | Kyung Seok Oh Jae Won Roh Sun Young Joo Kunhi Ryu Jung Ah Kim Se Jin Kim Seung Hyun Jang Young Ik Koh Da Hye Kim Hye-Youn Kim Murim Choi Jinsei Jung Wan Namkung Joo Hyun Nam Jae Young Choi Heon Yung Gee |
| author_sort | Kyung Seok Oh |
| collection | DOAJ |
| description | Abstract Pathogenic variants of KCNQ4 cause symmetrical, late-onset, progressive, high-frequency-affected hearing loss, which eventually involves all frequencies with age. To understand the contribution of KCNQ4 variants to hearing loss, we analyzed whole-exome and genome sequencing data from patients with hearing loss and individuals whose hearing phenotypes were unknown. In KCNQ4, we identified seven missense variants and one deletion variant in 9 hearing loss patients and 14 missense variants in the Korean population with an unknown hearing loss phenotype. The p.R420W and p.R447W variants were found in both cohorts. To investigate the effects of these variants on KCNQ4 function, we performed whole-cell patch clamping and examined their expression levels. Except for p.G435Afs*61, all KCNQ4 variants exhibited normal expression patterns similar to those of wild-type KCNQ4. The p.R331Q, p.R331W, p.G435Afs*61, and p.S691G variants, which were identified in patients with hearing loss, showed a potassium (K+) current density lower than or similar to that of p.L47P, a previously reported pathogenic variant. The p.S185W and p.R216H variants shifted the activation voltage to hyperpolarized voltages. The channel activity of the p.S185W, p.R216H, p.V672M, and p.S691G KCNQ4 proteins was rescued by the KCNQ activators retigabine or zinc pyrithione, whereas p.G435Afs*61 KCNQ4 proteins were partially rescued by sodium butyrate, a chemical chaperone. Additionally, the structure of the variants predicted using AlphaFold2 showed impaired pore configurations, as did the patch-clamp data. Our findings suggest that KCNQ4 variants may be overlooked in hearing loss that starts in adulthood. Some of these variants are medically treatable; hence, genetic screening for KCNQ4 is important. |
| first_indexed | 2024-04-09T12:51:39Z |
| format | Article |
| id | doaj.art-703f8133399a488b88c67aab5d9e1a50 |
| institution | Directory Open Access Journal |
| issn | 2092-6413 |
| language | English |
| last_indexed | 2024-04-09T12:51:39Z |
| publishDate | 2023-04-01 |
| publisher | Nature Publishing Group |
| record_format | Article |
| series | Experimental and Molecular Medicine |
| spelling | doaj.art-703f8133399a488b88c67aab5d9e1a502023-05-14T11:09:41ZengNature Publishing GroupExperimental and Molecular Medicine2092-64132023-04-0155484485910.1038/s12276-023-00976-4Overlooked KCNQ4 variants augment the risk of hearing lossKyung Seok Oh0Jae Won Roh1Sun Young Joo2Kunhi Ryu3Jung Ah Kim4Se Jin Kim5Seung Hyun Jang6Young Ik Koh7Da Hye Kim8Hye-Youn Kim9Murim Choi10Jinsei Jung11Wan Namkung12Joo Hyun Nam13Jae Young Choi14Heon Yung Gee15Department of Pharmacology, Graduate School of Medical Science, Yonsei University College of MedicineDepartment of Pharmacology, Graduate School of Medical Science, Yonsei University College of MedicineDepartment of Pharmacology, Graduate School of Medical Science, Yonsei University College of MedicineYonsei University College of PharmacyDepartment of Pharmacology, Graduate School of Medical Science, Yonsei University College of MedicineDepartment of Pharmacology, Graduate School of Medical Science, Yonsei University College of MedicineDepartment of Pharmacology, Graduate School of Medical Science, Yonsei University College of MedicineDepartment of Pharmacology, Graduate School of Medical Science, Yonsei University College of MedicineDepartment of Otorhinolaryngology, Yonsei University College of MedicineDepartment of Pharmacology, Graduate School of Medical Science, Yonsei University College of MedicineDepartment of Biomedical Sciences, Seoul National University College of MedicineDepartment of Otorhinolaryngology, Yonsei University College of MedicineYonsei University College of PharmacyDepartment of Physiology, Dongguk University College of MedicineDepartment of Otorhinolaryngology, Yonsei University College of MedicineDepartment of Pharmacology, Graduate School of Medical Science, Yonsei University College of MedicineAbstract Pathogenic variants of KCNQ4 cause symmetrical, late-onset, progressive, high-frequency-affected hearing loss, which eventually involves all frequencies with age. To understand the contribution of KCNQ4 variants to hearing loss, we analyzed whole-exome and genome sequencing data from patients with hearing loss and individuals whose hearing phenotypes were unknown. In KCNQ4, we identified seven missense variants and one deletion variant in 9 hearing loss patients and 14 missense variants in the Korean population with an unknown hearing loss phenotype. The p.R420W and p.R447W variants were found in both cohorts. To investigate the effects of these variants on KCNQ4 function, we performed whole-cell patch clamping and examined their expression levels. Except for p.G435Afs*61, all KCNQ4 variants exhibited normal expression patterns similar to those of wild-type KCNQ4. The p.R331Q, p.R331W, p.G435Afs*61, and p.S691G variants, which were identified in patients with hearing loss, showed a potassium (K+) current density lower than or similar to that of p.L47P, a previously reported pathogenic variant. The p.S185W and p.R216H variants shifted the activation voltage to hyperpolarized voltages. The channel activity of the p.S185W, p.R216H, p.V672M, and p.S691G KCNQ4 proteins was rescued by the KCNQ activators retigabine or zinc pyrithione, whereas p.G435Afs*61 KCNQ4 proteins were partially rescued by sodium butyrate, a chemical chaperone. Additionally, the structure of the variants predicted using AlphaFold2 showed impaired pore configurations, as did the patch-clamp data. Our findings suggest that KCNQ4 variants may be overlooked in hearing loss that starts in adulthood. Some of these variants are medically treatable; hence, genetic screening for KCNQ4 is important.https://doi.org/10.1038/s12276-023-00976-4 |
| spellingShingle | Kyung Seok Oh Jae Won Roh Sun Young Joo Kunhi Ryu Jung Ah Kim Se Jin Kim Seung Hyun Jang Young Ik Koh Da Hye Kim Hye-Youn Kim Murim Choi Jinsei Jung Wan Namkung Joo Hyun Nam Jae Young Choi Heon Yung Gee Overlooked KCNQ4 variants augment the risk of hearing loss Experimental and Molecular Medicine |
| title | Overlooked KCNQ4 variants augment the risk of hearing loss |
| title_full | Overlooked KCNQ4 variants augment the risk of hearing loss |
| title_fullStr | Overlooked KCNQ4 variants augment the risk of hearing loss |
| title_full_unstemmed | Overlooked KCNQ4 variants augment the risk of hearing loss |
| title_short | Overlooked KCNQ4 variants augment the risk of hearing loss |
| title_sort | overlooked kcnq4 variants augment the risk of hearing loss |
| url | https://doi.org/10.1038/s12276-023-00976-4 |
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