An eDNA‐based assessment of Garra cambodgiensis (stonelapping minnow) distribution on a megadiverse river, the Mekong
Abstract Garra cambodgiensis (stonelapping minnow) has experienced significant population declines, prompting intensive research and management, although its distribution in river systems such as the Mekong remains obscure. Effective conservation and management necessitate accurate monitoring and su...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Wiley
2024-02-01
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| Series: | Ecology and Evolution |
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| Online Access: | https://doi.org/10.1002/ece3.10898 |
| _version_ | 1797290903610589184 |
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| author | Maslin Osathanunkul Chatmongkon Suwannapoom |
| author_facet | Maslin Osathanunkul Chatmongkon Suwannapoom |
| author_sort | Maslin Osathanunkul |
| collection | DOAJ |
| description | Abstract Garra cambodgiensis (stonelapping minnow) has experienced significant population declines, prompting intensive research and management, although its distribution in river systems such as the Mekong remains obscure. Effective conservation and management necessitate accurate monitoring and survey data on the distribution of freshwater species. Traditional surveying techniques for fish may be challenging and generate insufficient data on species distribution. This study developed an eDNA‐based method for detecting G. cambodgiensis to address this void. Twenty‐one locations were surveyed. Water samples were collected in triplicate from the river's surface at each site and processed within 48 h in a dedicated laboratory. Primers and probes for G. cambodgiensis were meticulously designed and species‐specificity tested to ensure accurate detection without interference from co‐occurring species in the same geographic range. Each water sample was analysed by qPCR using six technical replicates. The results of qPCR were reported as positive with quantifiable eDNA concentration (copies/mL), below the limit of quantification, or non‐detectable. G. cambodgiensis eDNA was detected in water samples collected from 10 out of 21 sampling sites, with concentrations ranging from 8.5 to 2990.0 copies/mL. Importantly, G. cambodgiensis eDNA was consistently detected in all three replicate water samples at each site where the qPCR experiment yielded positive results. The findings of this study demonstrate the feasibility and effectiveness of incorporating eDNA‐based monitoring or surveys for G. cambodgiensis in the ecologically diverse Mekong River. Monitoring based on eDNA can aid in targeting and informing conservation and management of G. cambodgiensis in its natural habitat. Comprehensive and robust information on species distribution can be obtained via an eDNA‐based survey, which could contribute to more efficient and informed decision‐making processes in fisheries management and conservation efforts. |
| first_indexed | 2024-03-07T19:28:59Z |
| format | Article |
| id | doaj.art-7042ecaea33c4e0e9c3fbd33db6224b3 |
| institution | Directory Open Access Journal |
| issn | 2045-7758 |
| language | English |
| last_indexed | 2024-03-07T19:28:59Z |
| publishDate | 2024-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | Ecology and Evolution |
| spelling | doaj.art-7042ecaea33c4e0e9c3fbd33db6224b32024-02-29T08:56:40ZengWileyEcology and Evolution2045-77582024-02-01142n/an/a10.1002/ece3.10898An eDNA‐based assessment of Garra cambodgiensis (stonelapping minnow) distribution on a megadiverse river, the MekongMaslin Osathanunkul0Chatmongkon Suwannapoom1Department of Biology, Faculty of Science Chiang Mai University Chiang Mai ThailandSchool of Agriculture and Natural Resources University of Phayao Phayao ThailandAbstract Garra cambodgiensis (stonelapping minnow) has experienced significant population declines, prompting intensive research and management, although its distribution in river systems such as the Mekong remains obscure. Effective conservation and management necessitate accurate monitoring and survey data on the distribution of freshwater species. Traditional surveying techniques for fish may be challenging and generate insufficient data on species distribution. This study developed an eDNA‐based method for detecting G. cambodgiensis to address this void. Twenty‐one locations were surveyed. Water samples were collected in triplicate from the river's surface at each site and processed within 48 h in a dedicated laboratory. Primers and probes for G. cambodgiensis were meticulously designed and species‐specificity tested to ensure accurate detection without interference from co‐occurring species in the same geographic range. Each water sample was analysed by qPCR using six technical replicates. The results of qPCR were reported as positive with quantifiable eDNA concentration (copies/mL), below the limit of quantification, or non‐detectable. G. cambodgiensis eDNA was detected in water samples collected from 10 out of 21 sampling sites, with concentrations ranging from 8.5 to 2990.0 copies/mL. Importantly, G. cambodgiensis eDNA was consistently detected in all three replicate water samples at each site where the qPCR experiment yielded positive results. The findings of this study demonstrate the feasibility and effectiveness of incorporating eDNA‐based monitoring or surveys for G. cambodgiensis in the ecologically diverse Mekong River. Monitoring based on eDNA can aid in targeting and informing conservation and management of G. cambodgiensis in its natural habitat. Comprehensive and robust information on species distribution can be obtained via an eDNA‐based survey, which could contribute to more efficient and informed decision‐making processes in fisheries management and conservation efforts.https://doi.org/10.1002/ece3.10898data‐poor areaeconomically important fishpopulation declinespecies occurrence and distributionspecies‐specific detectionthe Mekong River |
| spellingShingle | Maslin Osathanunkul Chatmongkon Suwannapoom An eDNA‐based assessment of Garra cambodgiensis (stonelapping minnow) distribution on a megadiverse river, the Mekong Ecology and Evolution data‐poor area economically important fish population decline species occurrence and distribution species‐specific detection the Mekong River |
| title | An eDNA‐based assessment of Garra cambodgiensis (stonelapping minnow) distribution on a megadiverse river, the Mekong |
| title_full | An eDNA‐based assessment of Garra cambodgiensis (stonelapping minnow) distribution on a megadiverse river, the Mekong |
| title_fullStr | An eDNA‐based assessment of Garra cambodgiensis (stonelapping minnow) distribution on a megadiverse river, the Mekong |
| title_full_unstemmed | An eDNA‐based assessment of Garra cambodgiensis (stonelapping minnow) distribution on a megadiverse river, the Mekong |
| title_short | An eDNA‐based assessment of Garra cambodgiensis (stonelapping minnow) distribution on a megadiverse river, the Mekong |
| title_sort | edna based assessment of garra cambodgiensis stonelapping minnow distribution on a megadiverse river the mekong |
| topic | data‐poor area economically important fish population decline species occurrence and distribution species‐specific detection the Mekong River |
| url | https://doi.org/10.1002/ece3.10898 |
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