Purification of Peptide Components including Melittin from Bee Venom using gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis
Objectives : This study was conducted to carry out Purification of Melittin and other peptide components from Bee Venom using gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis Methods : Melittin and other peptide components were separated from bee venom by usi...
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| Format: | Article |
| Language: | English |
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Korean Pharmacopuncture Institute
2006-06-01
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| Series: | Journal of Pharmacopuncture |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.3831/KPI.2006.9.2.105 |
| _version_ | 1811244452646223872 |
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| author | Young Chon Choi Ki Rok, Kwon Suk Ho, Choi |
| author_facet | Young Chon Choi Ki Rok, Kwon Suk Ho, Choi |
| author_sort | Young Chon Choi |
| collection | DOAJ |
| description | Objectives : This study was conducted to carry out Purification of Melittin and other peptide components from Bee Venom using gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis
Methods : Melittin and other peptide components were separated from bee venom by using gel filtration chromatography on Sephadex G-50 column in 0.05M ammonium acetate buffer.
Results : Melittin and other peptide components were separated from bee venom by using gel filtration chromatography on Sephadex G-50 column in 0.05M ammonium acetate buffer. The fractions obtained from gel filtration chromatography was analyzed by using SDS-PAGE and propionic acid/urea polyacrylamide gel electrophoresis. The melittin obtained from the gel filtration contained residual amount of phospholipase A2 and a protein with molecular weight of 6,000. The contaminating proteins were removed by the second gel filtration chromatography.
Conclusion : Gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis are useful to separate peptide components including melittin from bee venom. |
| first_indexed | 2024-04-12T14:25:17Z |
| format | Article |
| id | doaj.art-7043fba64671400b9b64cb5156f719c1 |
| institution | Directory Open Access Journal |
| issn | 2093-6966 2234-6856 |
| language | English |
| last_indexed | 2024-04-12T14:25:17Z |
| publishDate | 2006-06-01 |
| publisher | Korean Pharmacopuncture Institute |
| record_format | Article |
| series | Journal of Pharmacopuncture |
| spelling | doaj.art-7043fba64671400b9b64cb5156f719c12022-12-22T03:29:27ZengKorean Pharmacopuncture InstituteJournal of Pharmacopuncture2093-69662234-68562006-06-019210511110.3831/KPI.2006.9.2.105Purification of Peptide Components including Melittin from Bee Venom using gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresisYoung Chon Choi0Ki Rok, Kwon1Suk Ho, Choi2Dept. of Acupuncture & Moxibustion, Oriental Medical College, Sangji UniversityDept. of Acupuncture & Moxibustion, Oriental Medical College, Sangji UniversityDevision of Animal resourses and life science, Sangji UniversityObjectives : This study was conducted to carry out Purification of Melittin and other peptide components from Bee Venom using gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis Methods : Melittin and other peptide components were separated from bee venom by using gel filtration chromatography on Sephadex G-50 column in 0.05M ammonium acetate buffer. Results : Melittin and other peptide components were separated from bee venom by using gel filtration chromatography on Sephadex G-50 column in 0.05M ammonium acetate buffer. The fractions obtained from gel filtration chromatography was analyzed by using SDS-PAGE and propionic acid/urea polyacrylamide gel electrophoresis. The melittin obtained from the gel filtration contained residual amount of phospholipase A2 and a protein with molecular weight of 6,000. The contaminating proteins were removed by the second gel filtration chromatography. Conclusion : Gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis are useful to separate peptide components including melittin from bee venom.http://dx.doi.org/10.3831/KPI.2006.9.2.105Gel filtration chromatographypolyacrylamide gel electrophoresismelittinbee venom |
| spellingShingle | Young Chon Choi Ki Rok, Kwon Suk Ho, Choi Purification of Peptide Components including Melittin from Bee Venom using gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis Journal of Pharmacopuncture Gel filtration chromatography polyacrylamide gel electrophoresis melittin bee venom |
| title | Purification of Peptide Components including Melittin from Bee Venom using gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis |
| title_full | Purification of Peptide Components including Melittin from Bee Venom using gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis |
| title_fullStr | Purification of Peptide Components including Melittin from Bee Venom using gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis |
| title_full_unstemmed | Purification of Peptide Components including Melittin from Bee Venom using gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis |
| title_short | Purification of Peptide Components including Melittin from Bee Venom using gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis |
| title_sort | purification of peptide components including melittin from bee venom using gel filtration chromatography and propionic acid urea polyacrylamide gel electrophoresis |
| topic | Gel filtration chromatography polyacrylamide gel electrophoresis melittin bee venom |
| url | http://dx.doi.org/10.3831/KPI.2006.9.2.105 |
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