Invasive terrestrial invertebrate detection in water and soil using a targeted eDNA approach
Terrestrial invasive invertebrates can rapidly colonise new areas, causing detrimental effects on biodiversity, economy and lifestyle. Targeted environmental DNA (eDNA) methods could constitute an early detection tool given their sensitivity to small numbers of individuals. We hypothesised that terr...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Pensoft Publishers
2023-04-01
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| Series: | NeoBiota |
| Online Access: | https://neobiota.pensoft.net/article/98898/download/pdf/ |
| _version_ | 1797851984675471360 |
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| author | Cecilia Villacorta-Rath Lori Lach Natalia Andrade-Rodriguez Damien Burrows Dianne Gleeson Alejandro Trujillo-González |
| author_facet | Cecilia Villacorta-Rath Lori Lach Natalia Andrade-Rodriguez Damien Burrows Dianne Gleeson Alejandro Trujillo-González |
| author_sort | Cecilia Villacorta-Rath |
| collection | DOAJ |
| description | Terrestrial invasive invertebrates can rapidly colonise new areas, causing detrimental effects on biodiversity, economy and lifestyle. Targeted environmental DNA (eDNA) methods could constitute an early detection tool given their sensitivity to small numbers of individuals. We hypothesised that terrestrial runoff would transport eDNA from the land into adjacent waterbodies and used the invasive yellow crazy ant (Anoplolepis gracilipes) as a model species to test this hypothesis. We collected water samples from four waterbodies adjacent (< 10 m from the creek edge) to infestations following rainfall events for eDNA analysis. We also collected soil samples from areas of known infestations and tested five eDNA extraction methods to determine their efficiency to extract eDNA from soil. Water samples resulted in positive yellow crazy ant eDNA amplification (20–100% field replicates across all sites), even at one site located 300 m away from where ants had been detected visually. Soil samples resulted in a higher percentage of false negatives when sampled from ant transit areas than from nest entrances. Unpurified DNA extracts from soil also resulted in false negative detections and only after applying a purification step of DNA extracts, did we detect yellow crazy ant eDNA in 40–100% of field replicates across all methods and sites. This is the first study to empirically show that eDNA from a terrestrial invertebrate can be successfully isolated and amplified from adjacent or downstream waterbodies. Our results indicate that eDNA has the potential to be a useful method for detecting terrestrial invertebrates from soil and water. |
| first_indexed | 2024-04-09T19:25:35Z |
| format | Article |
| id | doaj.art-70456657f0cf4b38adaea3d16c186092 |
| institution | Directory Open Access Journal |
| issn | 1314-2488 |
| language | English |
| last_indexed | 2024-04-09T19:25:35Z |
| publishDate | 2023-04-01 |
| publisher | Pensoft Publishers |
| record_format | Article |
| series | NeoBiota |
| spelling | doaj.art-70456657f0cf4b38adaea3d16c1860922023-04-05T08:11:04ZengPensoft PublishersNeoBiota1314-24882023-04-0183718910.3897/neobiota.83.9889898898Invasive terrestrial invertebrate detection in water and soil using a targeted eDNA approachCecilia Villacorta-Rath0Lori Lach1Natalia Andrade-Rodriguez2Damien Burrows3Dianne Gleeson4Alejandro Trujillo-González5James Cook UniversityJames Cook UniversityJames Cook UniversityJames Cook UniversityUniversity of CanberraUniversity of CanberraTerrestrial invasive invertebrates can rapidly colonise new areas, causing detrimental effects on biodiversity, economy and lifestyle. Targeted environmental DNA (eDNA) methods could constitute an early detection tool given their sensitivity to small numbers of individuals. We hypothesised that terrestrial runoff would transport eDNA from the land into adjacent waterbodies and used the invasive yellow crazy ant (Anoplolepis gracilipes) as a model species to test this hypothesis. We collected water samples from four waterbodies adjacent (< 10 m from the creek edge) to infestations following rainfall events for eDNA analysis. We also collected soil samples from areas of known infestations and tested five eDNA extraction methods to determine their efficiency to extract eDNA from soil. Water samples resulted in positive yellow crazy ant eDNA amplification (20–100% field replicates across all sites), even at one site located 300 m away from where ants had been detected visually. Soil samples resulted in a higher percentage of false negatives when sampled from ant transit areas than from nest entrances. Unpurified DNA extracts from soil also resulted in false negative detections and only after applying a purification step of DNA extracts, did we detect yellow crazy ant eDNA in 40–100% of field replicates across all methods and sites. This is the first study to empirically show that eDNA from a terrestrial invertebrate can be successfully isolated and amplified from adjacent or downstream waterbodies. Our results indicate that eDNA has the potential to be a useful method for detecting terrestrial invertebrates from soil and water.https://neobiota.pensoft.net/article/98898/download/pdf/ |
| spellingShingle | Cecilia Villacorta-Rath Lori Lach Natalia Andrade-Rodriguez Damien Burrows Dianne Gleeson Alejandro Trujillo-González Invasive terrestrial invertebrate detection in water and soil using a targeted eDNA approach NeoBiota |
| title | Invasive terrestrial invertebrate detection in water and soil using a targeted eDNA approach |
| title_full | Invasive terrestrial invertebrate detection in water and soil using a targeted eDNA approach |
| title_fullStr | Invasive terrestrial invertebrate detection in water and soil using a targeted eDNA approach |
| title_full_unstemmed | Invasive terrestrial invertebrate detection in water and soil using a targeted eDNA approach |
| title_short | Invasive terrestrial invertebrate detection in water and soil using a targeted eDNA approach |
| title_sort | invasive terrestrial invertebrate detection in water and soil using a targeted edna approach |
| url | https://neobiota.pensoft.net/article/98898/download/pdf/ |
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