Evaluation of the antibacterial activity of Lactobacilli probiotics supernatants against Enterococcus faecalis (in-vitro study)
Abstract Background There is an increasing demand to find a potent antibacterial agent against endodontic pathogens with the least toxic effect. The study aimed to evaluate the antibacterial activity of Lactobacilli probiotics on Enterococcus faecalis (E. faecalis) in comparison to calcium hydroxide...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-09-01
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| Series: | BMC Oral Health |
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| Online Access: | https://doi.org/10.1186/s12903-022-02434-5 |
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| author | Shymaa Shaaban Gamal M. Hamad Salma Genena Marwa A. Meheissen Sybel Moussa |
| author_facet | Shymaa Shaaban Gamal M. Hamad Salma Genena Marwa A. Meheissen Sybel Moussa |
| author_sort | Shymaa Shaaban |
| collection | DOAJ |
| description | Abstract Background There is an increasing demand to find a potent antibacterial agent against endodontic pathogens with the least toxic effect. The study aimed to evaluate the antibacterial activity of Lactobacilli probiotics on Enterococcus faecalis (E. faecalis) in comparison to calcium hydroxide paste. Methods The study involved two stages; (stage one): determination of the antibacterial properties of three strains of Lactobacilli probiotics supernatants (PS); Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus acidophilus, and a cocktail mix of the three supernatants against E. faecalis using agar well diffusion method in both solution and gel phase. The formed zones of inhibition (ZOI) were measured in millimeters (mm) and compared to each other. PS solution and gel of the largest ZOI were further compared with calcium hydroxide paste (35% Ultra Cal XS Ca(OH)2) This was followed by (stage two): assessment of the minimum inhibitory concentration (MIC) of the PS that showed the largest ZOI against E. faecalis by agar well diffusion assay for both PS solution and gel. Results All supernatants showed growth inhibition against E. faecalis, and the cocktail mix showed the largest ZOI. However, no significant difference was found between the supernatants in both the solution and gel phases (p > 0.05). Ca(OH)2 showed a significantly lower effect than both the cocktail mix solution and gel (p < 0.05). The MIC of the cocktail mix solution and gel against E. faecalis was 50 mg/ml. (p > 0.05). Conclusion PS has an antibacterial effect on E. faecalis and was more effective than Ca(OH)2. Lactobacilli probiotics could be a promising antibacterial agent used as an irrigant or an intracanal medication. |
| first_indexed | 2024-04-14T07:15:56Z |
| format | Article |
| id | doaj.art-70457aa881e344ca87b09aeba99425f8 |
| institution | Directory Open Access Journal |
| issn | 1472-6831 |
| language | English |
| last_indexed | 2024-04-14T07:15:56Z |
| publishDate | 2022-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Oral Health |
| spelling | doaj.art-70457aa881e344ca87b09aeba99425f82022-12-22T02:06:18ZengBMCBMC Oral Health1472-68312022-09-0122111110.1186/s12903-022-02434-5Evaluation of the antibacterial activity of Lactobacilli probiotics supernatants against Enterococcus faecalis (in-vitro study)Shymaa Shaaban0Gamal M. Hamad1Salma Genena2Marwa A. Meheissen3Sybel Moussa4Division of Endodontics, Conservative Dentistry Department, Faculty of Dentistry, Alexandria UniversityDepartment of Food Technology, Arid Lands Cultivation Research Institute (ALCRI), City of Scientific Research and Technological Applications (SRTACity)Division of Endodontics, Conservative Dentistry Department, Faculty of Dentistry, Alexandria UniversityMedical Microbiology and Immunology Department, Faculty of Medicine, Alexandria UniversityDivision of Endodontics, Conservative Dentistry Department, Faculty of Dentistry, Alexandria UniversityAbstract Background There is an increasing demand to find a potent antibacterial agent against endodontic pathogens with the least toxic effect. The study aimed to evaluate the antibacterial activity of Lactobacilli probiotics on Enterococcus faecalis (E. faecalis) in comparison to calcium hydroxide paste. Methods The study involved two stages; (stage one): determination of the antibacterial properties of three strains of Lactobacilli probiotics supernatants (PS); Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus acidophilus, and a cocktail mix of the three supernatants against E. faecalis using agar well diffusion method in both solution and gel phase. The formed zones of inhibition (ZOI) were measured in millimeters (mm) and compared to each other. PS solution and gel of the largest ZOI were further compared with calcium hydroxide paste (35% Ultra Cal XS Ca(OH)2) This was followed by (stage two): assessment of the minimum inhibitory concentration (MIC) of the PS that showed the largest ZOI against E. faecalis by agar well diffusion assay for both PS solution and gel. Results All supernatants showed growth inhibition against E. faecalis, and the cocktail mix showed the largest ZOI. However, no significant difference was found between the supernatants in both the solution and gel phases (p > 0.05). Ca(OH)2 showed a significantly lower effect than both the cocktail mix solution and gel (p < 0.05). The MIC of the cocktail mix solution and gel against E. faecalis was 50 mg/ml. (p > 0.05). Conclusion PS has an antibacterial effect on E. faecalis and was more effective than Ca(OH)2. Lactobacilli probiotics could be a promising antibacterial agent used as an irrigant or an intracanal medication.https://doi.org/10.1186/s12903-022-02434-5Calcium hydroxideEndodonticsEnterococcus faecalisLactobacilliProbiotics |
| spellingShingle | Shymaa Shaaban Gamal M. Hamad Salma Genena Marwa A. Meheissen Sybel Moussa Evaluation of the antibacterial activity of Lactobacilli probiotics supernatants against Enterococcus faecalis (in-vitro study) BMC Oral Health Calcium hydroxide Endodontics Enterococcus faecalis Lactobacilli Probiotics |
| title | Evaluation of the antibacterial activity of Lactobacilli probiotics supernatants against Enterococcus faecalis (in-vitro study) |
| title_full | Evaluation of the antibacterial activity of Lactobacilli probiotics supernatants against Enterococcus faecalis (in-vitro study) |
| title_fullStr | Evaluation of the antibacterial activity of Lactobacilli probiotics supernatants against Enterococcus faecalis (in-vitro study) |
| title_full_unstemmed | Evaluation of the antibacterial activity of Lactobacilli probiotics supernatants against Enterococcus faecalis (in-vitro study) |
| title_short | Evaluation of the antibacterial activity of Lactobacilli probiotics supernatants against Enterococcus faecalis (in-vitro study) |
| title_sort | evaluation of the antibacterial activity of lactobacilli probiotics supernatants against enterococcus faecalis in vitro study |
| topic | Calcium hydroxide Endodontics Enterococcus faecalis Lactobacilli Probiotics |
| url | https://doi.org/10.1186/s12903-022-02434-5 |
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