Silk fibroin micro-particle scaffolds with superior compression modulus and slow bioresorption for effective bone regeneration
Abstract Silk fibroin (SF), a natural polymer produced by Bombyx mori silkworms, has been extensively explored to prepare porous scaffolds for tissue engineering applications. Here, we demonstrate, a scaffold made of SF, which exhibits compression modulus comparable to natural cancellous bone while...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2018-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-018-25643-x |
| _version_ | 1828974933669052416 |
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| author | Anuya Nisal Raeesa Sayyad Prachi Dhavale Bhakti Khude Rucha Deshpande Vidhyashri Mapare Swati Shukla Premnath Venugopalan |
| author_facet | Anuya Nisal Raeesa Sayyad Prachi Dhavale Bhakti Khude Rucha Deshpande Vidhyashri Mapare Swati Shukla Premnath Venugopalan |
| author_sort | Anuya Nisal |
| collection | DOAJ |
| description | Abstract Silk fibroin (SF), a natural polymer produced by Bombyx mori silkworms, has been extensively explored to prepare porous scaffolds for tissue engineering applications. Here, we demonstrate, a scaffold made of SF, which exhibits compression modulus comparable to natural cancellous bone while retaining the appropriate porosities and interconnected pore architecture. The scaffolds also exhibit high resistance to in-vitro proteolytic degradation due to the dominant beta sheet conformation of the SF protein. Additionally, the scaffolds are prepared using a simple method of microparticle aggregation. We also demonstrate, for the first time, a method to prepare SF micro-particles using a Hexafluoroisopropanol-Methanol solvent-coagulant combination. SF microparticles obtained using this method are monodisperse, spherical, non-porous and extremely crystalline. These micro-particles have been further aggregated together to form a 3D scaffold. The aggregation is achieved by random packing of these microparticles and fusing them together using a dilute SF solution. Preliminary in-vitro cell culture and in-vivo implantation studies demonstrate that the scaffolds are biocompatible and they exhibit the appropriate early markers, making them promising candidates for bone regeneration. |
| first_indexed | 2024-12-14T14:12:23Z |
| format | Article |
| id | doaj.art-704930cc48c2457f8c58276fe8eea5c7 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T14:12:23Z |
| publishDate | 2018-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-704930cc48c2457f8c58276fe8eea5c72022-12-21T22:58:17ZengNature PortfolioScientific Reports2045-23222018-05-018111010.1038/s41598-018-25643-xSilk fibroin micro-particle scaffolds with superior compression modulus and slow bioresorption for effective bone regenerationAnuya Nisal0Raeesa Sayyad1Prachi Dhavale2Bhakti Khude3Rucha Deshpande4Vidhyashri Mapare5Swati Shukla6Premnath Venugopalan7Polymer Science and Engineering Department, National Chemical LaboratoryBiolMed Innovations Pvt. Ltd., 100, NCL Innovation ParkPolymer Science and Engineering Department, National Chemical LaboratoryPolymer Science and Engineering Department, National Chemical LaboratoryBiolMed Innovations Pvt. Ltd., 100, NCL Innovation ParkBiolMed Innovations Pvt. Ltd., 100, NCL Innovation ParkBiolMed Innovations Pvt. Ltd., 100, NCL Innovation ParkPolymer Science and Engineering Department, National Chemical LaboratoryAbstract Silk fibroin (SF), a natural polymer produced by Bombyx mori silkworms, has been extensively explored to prepare porous scaffolds for tissue engineering applications. Here, we demonstrate, a scaffold made of SF, which exhibits compression modulus comparable to natural cancellous bone while retaining the appropriate porosities and interconnected pore architecture. The scaffolds also exhibit high resistance to in-vitro proteolytic degradation due to the dominant beta sheet conformation of the SF protein. Additionally, the scaffolds are prepared using a simple method of microparticle aggregation. We also demonstrate, for the first time, a method to prepare SF micro-particles using a Hexafluoroisopropanol-Methanol solvent-coagulant combination. SF microparticles obtained using this method are monodisperse, spherical, non-porous and extremely crystalline. These micro-particles have been further aggregated together to form a 3D scaffold. The aggregation is achieved by random packing of these microparticles and fusing them together using a dilute SF solution. Preliminary in-vitro cell culture and in-vivo implantation studies demonstrate that the scaffolds are biocompatible and they exhibit the appropriate early markers, making them promising candidates for bone regeneration.https://doi.org/10.1038/s41598-018-25643-x |
| spellingShingle | Anuya Nisal Raeesa Sayyad Prachi Dhavale Bhakti Khude Rucha Deshpande Vidhyashri Mapare Swati Shukla Premnath Venugopalan Silk fibroin micro-particle scaffolds with superior compression modulus and slow bioresorption for effective bone regeneration Scientific Reports |
| title | Silk fibroin micro-particle scaffolds with superior compression modulus and slow bioresorption for effective bone regeneration |
| title_full | Silk fibroin micro-particle scaffolds with superior compression modulus and slow bioresorption for effective bone regeneration |
| title_fullStr | Silk fibroin micro-particle scaffolds with superior compression modulus and slow bioresorption for effective bone regeneration |
| title_full_unstemmed | Silk fibroin micro-particle scaffolds with superior compression modulus and slow bioresorption for effective bone regeneration |
| title_short | Silk fibroin micro-particle scaffolds with superior compression modulus and slow bioresorption for effective bone regeneration |
| title_sort | silk fibroin micro particle scaffolds with superior compression modulus and slow bioresorption for effective bone regeneration |
| url | https://doi.org/10.1038/s41598-018-25643-x |
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