Interaction of Lipoplex with Albumin Enhances Gene Expression in Hepatitis Mice
Understanding the in vivo fate of lipoplex, which is composed of cationic liposomes and DNA, is an important issue toward gene therapy. In disease conditions, the fate of lipoplex might change compared with the normal condition. Here, we examined the contribution of interaction with serum components...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-04-01
|
| Series: | Pharmaceutics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4923/12/4/341 |
| _version_ | 1797570968343805952 |
|---|---|
| author | Naoki Yoshikawa Shintaro Fumoto Keiko Yoshikawa Die Hu Kazuya Okami Riku Kato Mikiro Nakashima Hirotaka Miyamoto Koyo Nishida |
| author_facet | Naoki Yoshikawa Shintaro Fumoto Keiko Yoshikawa Die Hu Kazuya Okami Riku Kato Mikiro Nakashima Hirotaka Miyamoto Koyo Nishida |
| author_sort | Naoki Yoshikawa |
| collection | DOAJ |
| description | Understanding the in vivo fate of lipoplex, which is composed of cationic liposomes and DNA, is an important issue toward gene therapy. In disease conditions, the fate of lipoplex might change compared with the normal condition. Here, we examined the contribution of interaction with serum components to in vivo transfection using lipoplex in hepatitis mice. Prior to administration, lipoplex was incubated with serum or albumin. In the liver, the interaction with albumin enhanced gene expression in hepatitis mice, while in the lung, the interaction with serum or albumin enhanced it. In normal mice, the interaction with albumin did not enhance hepatic and pulmonary gene expression. Furthermore, hepatic and pulmonary gene expression levels of albumin-interacted lipoplex were correlated with serum transaminases in hepatitis mice. The albumin interaction increased the hepatic accumulation of lipoplex and serum tumor necrosis factor-α level. We suggest that the interaction with albumin enhanced the inflammation level after the administration of lipoplex in hepatitis mice. Consequently, the enhancement of the inflammation level might enhance the gene expression level. Information obtained in the current study will be valuable toward future clinical application of the lipoplex. |
| first_indexed | 2024-03-10T20:32:59Z |
| format | Article |
| id | doaj.art-7049a3e3922e44afae95f379d6ba8888 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T20:32:59Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-7049a3e3922e44afae95f379d6ba88882023-11-19T21:15:49ZengMDPI AGPharmaceutics1999-49232020-04-0112434110.3390/pharmaceutics12040341Interaction of Lipoplex with Albumin Enhances Gene Expression in Hepatitis MiceNaoki Yoshikawa0Shintaro Fumoto1Keiko Yoshikawa2Die Hu3Kazuya Okami4Riku Kato5Mikiro Nakashima6Hirotaka Miyamoto7Koyo Nishida8Department of Pharmacy, University of Miyazaki Hospital, 5200 Kihara, Kiyotake-cho, Miyazaki 889-1692, JapanGraduate School of Biomedical Sciences, Nagasaki University, 1-7-1 Sakamoto, Nagasaki-shi, Nagasaki 852-8501, JapanGraduate School of Biomedical Sciences, Nagasaki University, 1-7-1 Sakamoto, Nagasaki-shi, Nagasaki 852-8501, JapanGraduate School of Biomedical Sciences, Nagasaki University, 1-7-1 Sakamoto, Nagasaki-shi, Nagasaki 852-8501, JapanGraduate School of Biomedical Sciences, Nagasaki University, 1-7-1 Sakamoto, Nagasaki-shi, Nagasaki 852-8501, JapanGraduate School of Biomedical Sciences, Nagasaki University, 1-7-1 Sakamoto, Nagasaki-shi, Nagasaki 852-8501, JapanGraduate School of Biomedical Sciences, Nagasaki University, 1-7-1 Sakamoto, Nagasaki-shi, Nagasaki 852-8501, JapanGraduate School of Biomedical Sciences, Nagasaki University, 1-7-1 Sakamoto, Nagasaki-shi, Nagasaki 852-8501, JapanGraduate School of Biomedical Sciences, Nagasaki University, 1-7-1 Sakamoto, Nagasaki-shi, Nagasaki 852-8501, JapanUnderstanding the in vivo fate of lipoplex, which is composed of cationic liposomes and DNA, is an important issue toward gene therapy. In disease conditions, the fate of lipoplex might change compared with the normal condition. Here, we examined the contribution of interaction with serum components to in vivo transfection using lipoplex in hepatitis mice. Prior to administration, lipoplex was incubated with serum or albumin. In the liver, the interaction with albumin enhanced gene expression in hepatitis mice, while in the lung, the interaction with serum or albumin enhanced it. In normal mice, the interaction with albumin did not enhance hepatic and pulmonary gene expression. Furthermore, hepatic and pulmonary gene expression levels of albumin-interacted lipoplex were correlated with serum transaminases in hepatitis mice. The albumin interaction increased the hepatic accumulation of lipoplex and serum tumor necrosis factor-α level. We suggest that the interaction with albumin enhanced the inflammation level after the administration of lipoplex in hepatitis mice. Consequently, the enhancement of the inflammation level might enhance the gene expression level. Information obtained in the current study will be valuable toward future clinical application of the lipoplex.https://www.mdpi.com/1999-4923/12/4/341in vivo gene transfectionlipofectionhepatitisinteraction with serum componentslungliver |
| spellingShingle | Naoki Yoshikawa Shintaro Fumoto Keiko Yoshikawa Die Hu Kazuya Okami Riku Kato Mikiro Nakashima Hirotaka Miyamoto Koyo Nishida Interaction of Lipoplex with Albumin Enhances Gene Expression in Hepatitis Mice Pharmaceutics in vivo gene transfection lipofection hepatitis interaction with serum components lung liver |
| title | Interaction of Lipoplex with Albumin Enhances Gene Expression in Hepatitis Mice |
| title_full | Interaction of Lipoplex with Albumin Enhances Gene Expression in Hepatitis Mice |
| title_fullStr | Interaction of Lipoplex with Albumin Enhances Gene Expression in Hepatitis Mice |
| title_full_unstemmed | Interaction of Lipoplex with Albumin Enhances Gene Expression in Hepatitis Mice |
| title_short | Interaction of Lipoplex with Albumin Enhances Gene Expression in Hepatitis Mice |
| title_sort | interaction of lipoplex with albumin enhances gene expression in hepatitis mice |
| topic | in vivo gene transfection lipofection hepatitis interaction with serum components lung liver |
| url | https://www.mdpi.com/1999-4923/12/4/341 |
| work_keys_str_mv | AT naokiyoshikawa interactionoflipoplexwithalbuminenhancesgeneexpressioninhepatitismice AT shintarofumoto interactionoflipoplexwithalbuminenhancesgeneexpressioninhepatitismice AT keikoyoshikawa interactionoflipoplexwithalbuminenhancesgeneexpressioninhepatitismice AT diehu interactionoflipoplexwithalbuminenhancesgeneexpressioninhepatitismice AT kazuyaokami interactionoflipoplexwithalbuminenhancesgeneexpressioninhepatitismice AT rikukato interactionoflipoplexwithalbuminenhancesgeneexpressioninhepatitismice AT mikironakashima interactionoflipoplexwithalbuminenhancesgeneexpressioninhepatitismice AT hirotakamiyamoto interactionoflipoplexwithalbuminenhancesgeneexpressioninhepatitismice AT koyonishida interactionoflipoplexwithalbuminenhancesgeneexpressioninhepatitismice |