Determination of apolipoproteins B-48 and B-100 in triglyceride-rich lipoproteins by analytical SDS-PAGE.
The present work describes a procedure for determining apolipoproteins (apo) B-100 and B-48 in subfractions of triglyceride-rich lipoproteins by analytical sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) with Coomassie staining. The chromogenicity of the two apoB species was fou...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1994-07-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520399740 |
| _version_ | 1819076019939704832 |
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| author | F Karpe A Hamsten |
| author_facet | F Karpe A Hamsten |
| author_sort | F Karpe |
| collection | DOAJ |
| description | The present work describes a procedure for determining apolipoproteins (apo) B-100 and B-48 in subfractions of triglyceride-rich lipoproteins by analytical sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) with Coomassie staining. The chromogenicity of the two apoB species was found to be almost equal, and independent of lipoprotein particle size. Both proteins were sensitive to overloading of the gel, which resulted in low dye uptake. This was particularly evident for apoB-48. The precision of this analytical SDS-PAGE-based procedure to determine the plasma concentrations of apoB-100 and B-48 in triglyceride-rich lipoproteins was found to be appreciably low (coefficients of variation ranging between 3.1 and 14.1%). |
| first_indexed | 2024-12-21T18:34:39Z |
| format | Article |
| id | doaj.art-704a449897c445108fd6993c5834021c |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-21T18:34:39Z |
| publishDate | 1994-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-704a449897c445108fd6993c5834021c2022-12-21T18:54:11ZengElsevierJournal of Lipid Research0022-22751994-07-0135713111317Determination of apolipoproteins B-48 and B-100 in triglyceride-rich lipoproteins by analytical SDS-PAGE.F Karpe0A Hamsten1Atherosclerosis Research Unit, King Gustaf V Research Institute, Stockholm, Sweden.Atherosclerosis Research Unit, King Gustaf V Research Institute, Stockholm, Sweden.The present work describes a procedure for determining apolipoproteins (apo) B-100 and B-48 in subfractions of triglyceride-rich lipoproteins by analytical sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) with Coomassie staining. The chromogenicity of the two apoB species was found to be almost equal, and independent of lipoprotein particle size. Both proteins were sensitive to overloading of the gel, which resulted in low dye uptake. This was particularly evident for apoB-48. The precision of this analytical SDS-PAGE-based procedure to determine the plasma concentrations of apoB-100 and B-48 in triglyceride-rich lipoproteins was found to be appreciably low (coefficients of variation ranging between 3.1 and 14.1%).http://www.sciencedirect.com/science/article/pii/S0022227520399740 |
| spellingShingle | F Karpe A Hamsten Determination of apolipoproteins B-48 and B-100 in triglyceride-rich lipoproteins by analytical SDS-PAGE. Journal of Lipid Research |
| title | Determination of apolipoproteins B-48 and B-100 in triglyceride-rich lipoproteins by analytical SDS-PAGE. |
| title_full | Determination of apolipoproteins B-48 and B-100 in triglyceride-rich lipoproteins by analytical SDS-PAGE. |
| title_fullStr | Determination of apolipoproteins B-48 and B-100 in triglyceride-rich lipoproteins by analytical SDS-PAGE. |
| title_full_unstemmed | Determination of apolipoproteins B-48 and B-100 in triglyceride-rich lipoproteins by analytical SDS-PAGE. |
| title_short | Determination of apolipoproteins B-48 and B-100 in triglyceride-rich lipoproteins by analytical SDS-PAGE. |
| title_sort | determination of apolipoproteins b 48 and b 100 in triglyceride rich lipoproteins by analytical sds page |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520399740 |
| work_keys_str_mv | AT fkarpe determinationofapolipoproteinsb48andb100intriglyceriderichlipoproteinsbyanalyticalsdspage AT ahamsten determinationofapolipoproteinsb48andb100intriglyceriderichlipoproteinsbyanalyticalsdspage |