Dental pulp stem cells retain mesenchymal phenotype despite differentiation toward retinal neuronal fate in vitro
Dental pulp stem cells (DPSCs) are an easily accessible, heterogenous source of mesenchymal stem cells (MSCs) that are derived from the neural crest. Evidence suggests that they have neurotrophic qualities in their undifferentiated state and can also be differentiated into neuronal and retinal cell...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmed.2022.821361/full |
| _version_ | 1811239348765458432 |
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| author | Aishwarya Balasankar Shu-Yi Claire Chan Venkata Pakala Sudheer Babu Gary Yam Goh Bee Tin Shweta Singhal Shweta Singhal Shweta Singhal |
| author_facet | Aishwarya Balasankar Shu-Yi Claire Chan Venkata Pakala Sudheer Babu Gary Yam Goh Bee Tin Shweta Singhal Shweta Singhal Shweta Singhal |
| author_sort | Aishwarya Balasankar |
| collection | DOAJ |
| description | Dental pulp stem cells (DPSCs) are an easily accessible, heterogenous source of mesenchymal stem cells (MSCs) that are derived from the neural crest. Evidence suggests that they have neurotrophic qualities in their undifferentiated state and can also be differentiated into neuronal and retinal cell types. There is growing interest in using DPSCs in cell-based therapies to treat glaucoma and blinding retinal diseases. However, careful characterization of these cells is necessary as direct intravitreal and subretinal MSC transplantation is known to lead to deleterious glial reaction and fibrosis. In this study, we provide evidence for the mesenchymal-predominant nature of DPSCs and show that DPSCs maintain their mesenchymal phenotype despite upregulating mature retinal markers under retinal differentiation conditions. CD56, which was previously thought to be a specific marker of neural crest lineage, is robustly co-expressed with mesenchymal markers and may not be adequate for isolating a subpopulation of neural crest cells in DPSCs. Therefore, identification of more specific markers is required to elucidate the heterogeneity of the population and to successfully isolate a putative neural stem cell population before DPSCs can be used for retinal therapy. |
| first_indexed | 2024-04-12T12:58:03Z |
| format | Article |
| id | doaj.art-704b832d88ea40279f96f9b72f6b3942 |
| institution | Directory Open Access Journal |
| issn | 2296-858X |
| language | English |
| last_indexed | 2024-04-12T12:58:03Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Medicine |
| spelling | doaj.art-704b832d88ea40279f96f9b72f6b39422022-12-22T03:32:15ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2022-10-01910.3389/fmed.2022.821361821361Dental pulp stem cells retain mesenchymal phenotype despite differentiation toward retinal neuronal fate in vitroAishwarya Balasankar0Shu-Yi Claire Chan1Venkata Pakala Sudheer Babu2Gary Yam3Goh Bee Tin4Shweta Singhal5Shweta Singhal6Shweta Singhal7Singapore Eye Research Institute, Singapore, SingaporeSingapore Eye Research Institute, Singapore, SingaporeSingapore Eye Research Institute, Singapore, SingaporeSingapore Eye Research Institute, Singapore, SingaporeNational Dental Centre Singapore, Singapore, SingaporeSingapore Eye Research Institute, Singapore, SingaporeSingapore National Eye Centre, Singapore, SingaporeDuke NUS Medical School, Singapore, SingaporeDental pulp stem cells (DPSCs) are an easily accessible, heterogenous source of mesenchymal stem cells (MSCs) that are derived from the neural crest. Evidence suggests that they have neurotrophic qualities in their undifferentiated state and can also be differentiated into neuronal and retinal cell types. There is growing interest in using DPSCs in cell-based therapies to treat glaucoma and blinding retinal diseases. However, careful characterization of these cells is necessary as direct intravitreal and subretinal MSC transplantation is known to lead to deleterious glial reaction and fibrosis. In this study, we provide evidence for the mesenchymal-predominant nature of DPSCs and show that DPSCs maintain their mesenchymal phenotype despite upregulating mature retinal markers under retinal differentiation conditions. CD56, which was previously thought to be a specific marker of neural crest lineage, is robustly co-expressed with mesenchymal markers and may not be adequate for isolating a subpopulation of neural crest cells in DPSCs. Therefore, identification of more specific markers is required to elucidate the heterogeneity of the population and to successfully isolate a putative neural stem cell population before DPSCs can be used for retinal therapy.https://www.frontiersin.org/articles/10.3389/fmed.2022.821361/fulldental pulp stem cellsmesenchymal stem cellsretinal ganglion cellsretinal differentiationretinal stem cell therapy |
| spellingShingle | Aishwarya Balasankar Shu-Yi Claire Chan Venkata Pakala Sudheer Babu Gary Yam Goh Bee Tin Shweta Singhal Shweta Singhal Shweta Singhal Dental pulp stem cells retain mesenchymal phenotype despite differentiation toward retinal neuronal fate in vitro Frontiers in Medicine dental pulp stem cells mesenchymal stem cells retinal ganglion cells retinal differentiation retinal stem cell therapy |
| title | Dental pulp stem cells retain mesenchymal phenotype despite differentiation toward retinal neuronal fate in vitro |
| title_full | Dental pulp stem cells retain mesenchymal phenotype despite differentiation toward retinal neuronal fate in vitro |
| title_fullStr | Dental pulp stem cells retain mesenchymal phenotype despite differentiation toward retinal neuronal fate in vitro |
| title_full_unstemmed | Dental pulp stem cells retain mesenchymal phenotype despite differentiation toward retinal neuronal fate in vitro |
| title_short | Dental pulp stem cells retain mesenchymal phenotype despite differentiation toward retinal neuronal fate in vitro |
| title_sort | dental pulp stem cells retain mesenchymal phenotype despite differentiation toward retinal neuronal fate in vitro |
| topic | dental pulp stem cells mesenchymal stem cells retinal ganglion cells retinal differentiation retinal stem cell therapy |
| url | https://www.frontiersin.org/articles/10.3389/fmed.2022.821361/full |
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