MicroRNA-4429 suppresses proliferation of prostate cancer cells by targeting distal-less homeobox 1 and inactivating the Wnt/β-catenin pathway
Abstract Background Emerging evidence suggests that microRNAs (miRNAs) play multiple roles in human cancers through regulating mRNAs and distinct pathways. This paper focused on the functions of miR-4429 in prostate cancer (PCa) progression and the molecules involved. Methods Expression of miR-4429...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-03-01
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| Series: | BMC Urology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12894-021-00810-x |
| _version_ | 1818416558057193472 |
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| author | Jinguo Wang Sheng Xie Jun Liu Tao Li Wanrong Wang Ziping Xie |
| author_facet | Jinguo Wang Sheng Xie Jun Liu Tao Li Wanrong Wang Ziping Xie |
| author_sort | Jinguo Wang |
| collection | DOAJ |
| description | Abstract Background Emerging evidence suggests that microRNAs (miRNAs) play multiple roles in human cancers through regulating mRNAs and distinct pathways. This paper focused on the functions of miR-4429 in prostate cancer (PCa) progression and the molecules involved. Methods Expression of miR-4429 in PCa tissues and cells was determined. Upregulation of miR-4429 was introduced in PCa cells to examine its role in the malignant behaviors of cells. The putative target mRNA of miR-4429 involved in PCa progression was predicted from a bioinformatic system and validated through luciferase assays. Overexpression of distal-less homeobox 1 (DLX1) was further induced in cells to validate its implication in miR-4429-mediated events. The activity of Wnt/β-catenin pathway was determined. Results miR-4429 was poorly expressed in PCa tissues and cells. Artificial upregulation of miR-4429 significantly reduced proliferation, growth, invasion, migration and resistance to death of cancer cells and inactivated the Wnt/β-catenin pathway. DLX1 mRNA was found as a target of miR-4429. Upregulation of DLX1 restored the malignant behaviors of PCa cells which were initially suppressed by miR-4429, and it activated the Wnt/β-catenin pathway. Conclusion Our study highlights that miR-4429 inhibits the growth of PCa cells by down-regulating DLX1 and inactivating the Wnt/β-catenin pathway. This finding may offer novel insights into PCa treatment. |
| first_indexed | 2024-12-14T11:52:47Z |
| format | Article |
| id | doaj.art-704c32e2890a4279a30fbdde65dd3428 |
| institution | Directory Open Access Journal |
| issn | 1471-2490 |
| language | English |
| last_indexed | 2024-12-14T11:52:47Z |
| publishDate | 2021-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Urology |
| spelling | doaj.art-704c32e2890a4279a30fbdde65dd34282022-12-21T23:02:14ZengBMCBMC Urology1471-24902021-03-0121111010.1186/s12894-021-00810-xMicroRNA-4429 suppresses proliferation of prostate cancer cells by targeting distal-less homeobox 1 and inactivating the Wnt/β-catenin pathwayJinguo Wang0Sheng Xie1Jun Liu2Tao Li3Wanrong Wang4Ziping Xie5Department of Andrology, Renmin Hospital, Hubei University of MedicineDepartment of Andrology, Renmin Hospital, Hubei University of MedicineDepartment of Andrology, Renmin Hospital, Hubei University of MedicineDepartment of Andrology, Renmin Hospital, Hubei University of MedicineDepartment of Andrology, Renmin Hospital, Hubei University of MedicineDepartment of Andrology, Renmin Hospital, Hubei University of MedicineAbstract Background Emerging evidence suggests that microRNAs (miRNAs) play multiple roles in human cancers through regulating mRNAs and distinct pathways. This paper focused on the functions of miR-4429 in prostate cancer (PCa) progression and the molecules involved. Methods Expression of miR-4429 in PCa tissues and cells was determined. Upregulation of miR-4429 was introduced in PCa cells to examine its role in the malignant behaviors of cells. The putative target mRNA of miR-4429 involved in PCa progression was predicted from a bioinformatic system and validated through luciferase assays. Overexpression of distal-less homeobox 1 (DLX1) was further induced in cells to validate its implication in miR-4429-mediated events. The activity of Wnt/β-catenin pathway was determined. Results miR-4429 was poorly expressed in PCa tissues and cells. Artificial upregulation of miR-4429 significantly reduced proliferation, growth, invasion, migration and resistance to death of cancer cells and inactivated the Wnt/β-catenin pathway. DLX1 mRNA was found as a target of miR-4429. Upregulation of DLX1 restored the malignant behaviors of PCa cells which were initially suppressed by miR-4429, and it activated the Wnt/β-catenin pathway. Conclusion Our study highlights that miR-4429 inhibits the growth of PCa cells by down-regulating DLX1 and inactivating the Wnt/β-catenin pathway. This finding may offer novel insights into PCa treatment.https://doi.org/10.1186/s12894-021-00810-xProstate cancerMicroRNA-4429Wnt/β-catenin pathwayDLX1Biological behavior |
| spellingShingle | Jinguo Wang Sheng Xie Jun Liu Tao Li Wanrong Wang Ziping Xie MicroRNA-4429 suppresses proliferation of prostate cancer cells by targeting distal-less homeobox 1 and inactivating the Wnt/β-catenin pathway BMC Urology Prostate cancer MicroRNA-4429 Wnt/β-catenin pathway DLX1 Biological behavior |
| title | MicroRNA-4429 suppresses proliferation of prostate cancer cells by targeting distal-less homeobox 1 and inactivating the Wnt/β-catenin pathway |
| title_full | MicroRNA-4429 suppresses proliferation of prostate cancer cells by targeting distal-less homeobox 1 and inactivating the Wnt/β-catenin pathway |
| title_fullStr | MicroRNA-4429 suppresses proliferation of prostate cancer cells by targeting distal-less homeobox 1 and inactivating the Wnt/β-catenin pathway |
| title_full_unstemmed | MicroRNA-4429 suppresses proliferation of prostate cancer cells by targeting distal-less homeobox 1 and inactivating the Wnt/β-catenin pathway |
| title_short | MicroRNA-4429 suppresses proliferation of prostate cancer cells by targeting distal-less homeobox 1 and inactivating the Wnt/β-catenin pathway |
| title_sort | microrna 4429 suppresses proliferation of prostate cancer cells by targeting distal less homeobox 1 and inactivating the wnt β catenin pathway |
| topic | Prostate cancer MicroRNA-4429 Wnt/β-catenin pathway DLX1 Biological behavior |
| url | https://doi.org/10.1186/s12894-021-00810-x |
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