Development of an Extracorporeal Perfusion Device for Small Animal Free Flaps.
Extracorporeal perfusion (ECP) might prolong the vital storage capabilities of composite free flaps, potentially opening a wide range of clinical applications. Aim of the study was the development a validated low-cost extracorporeal perfusion model for further research in small animal free flaps.Aft...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4726627?pdf=render |
| _version_ | 1818278350461861888 |
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| author | Andreas M Fichter Lucas M Ritschl Anna Borgmann Martin Humbs Peter B Luppa Klaus-Dietrich Wolff Thomas Mücke |
| author_facet | Andreas M Fichter Lucas M Ritschl Anna Borgmann Martin Humbs Peter B Luppa Klaus-Dietrich Wolff Thomas Mücke |
| author_sort | Andreas M Fichter |
| collection | DOAJ |
| description | Extracorporeal perfusion (ECP) might prolong the vital storage capabilities of composite free flaps, potentially opening a wide range of clinical applications. Aim of the study was the development a validated low-cost extracorporeal perfusion model for further research in small animal free flaps.After establishing optimal perfusion settings, a specially designed extracorporeal perfusion system was evaluated during 8-hour perfusion of rat epigastric flaps followed by microvascular free flap transfer. Controls comprised sham-operation, ischemia and in vivo perfusion. Flaps and perfusate (diluted blood) were closely monitored by blood gas analysis, combined laser Doppler flowmetry and remission spectroscopy and Indocyanine-Green angiography. Evaluations were complemented by assessment of necrotic area and light microscopy at day 7.ECP was established and maintained for 8 hours with constant potassium and pH levels. Subsequent flap transfer was successful. Notably, the rate of necrosis of extracorporeally perfused flaps (27%) was even lower than after in vivo perfusion (49%), although not statistically significant (P = 0,083). After sham-operation, only 6% of the total flap area became necrotic, while 8-hour ischemia led to total flap loss (98%). Angiographic and histological findings confirmed these observations.Vital storage capabilities of microvascular flaps can be prolonged by temporary ECP. Our study provides important insights on the pathophysiological processes during extracorporeal tissue perfusion and provides a validated small animal perfusion model for further studies. |
| first_indexed | 2024-12-12T23:16:02Z |
| format | Article |
| id | doaj.art-704cbab1a8ca49b5a3f4bc4fb0f4e43d |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T23:16:02Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-704cbab1a8ca49b5a3f4bc4fb0f4e43d2022-12-22T00:08:27ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01111e014775510.1371/journal.pone.0147755Development of an Extracorporeal Perfusion Device for Small Animal Free Flaps.Andreas M FichterLucas M RitschlAnna BorgmannMartin HumbsPeter B LuppaKlaus-Dietrich WolffThomas MückeExtracorporeal perfusion (ECP) might prolong the vital storage capabilities of composite free flaps, potentially opening a wide range of clinical applications. Aim of the study was the development a validated low-cost extracorporeal perfusion model for further research in small animal free flaps.After establishing optimal perfusion settings, a specially designed extracorporeal perfusion system was evaluated during 8-hour perfusion of rat epigastric flaps followed by microvascular free flap transfer. Controls comprised sham-operation, ischemia and in vivo perfusion. Flaps and perfusate (diluted blood) were closely monitored by blood gas analysis, combined laser Doppler flowmetry and remission spectroscopy and Indocyanine-Green angiography. Evaluations were complemented by assessment of necrotic area and light microscopy at day 7.ECP was established and maintained for 8 hours with constant potassium and pH levels. Subsequent flap transfer was successful. Notably, the rate of necrosis of extracorporeally perfused flaps (27%) was even lower than after in vivo perfusion (49%), although not statistically significant (P = 0,083). After sham-operation, only 6% of the total flap area became necrotic, while 8-hour ischemia led to total flap loss (98%). Angiographic and histological findings confirmed these observations.Vital storage capabilities of microvascular flaps can be prolonged by temporary ECP. Our study provides important insights on the pathophysiological processes during extracorporeal tissue perfusion and provides a validated small animal perfusion model for further studies.http://europepmc.org/articles/PMC4726627?pdf=render |
| spellingShingle | Andreas M Fichter Lucas M Ritschl Anna Borgmann Martin Humbs Peter B Luppa Klaus-Dietrich Wolff Thomas Mücke Development of an Extracorporeal Perfusion Device for Small Animal Free Flaps. PLoS ONE |
| title | Development of an Extracorporeal Perfusion Device for Small Animal Free Flaps. |
| title_full | Development of an Extracorporeal Perfusion Device for Small Animal Free Flaps. |
| title_fullStr | Development of an Extracorporeal Perfusion Device for Small Animal Free Flaps. |
| title_full_unstemmed | Development of an Extracorporeal Perfusion Device for Small Animal Free Flaps. |
| title_short | Development of an Extracorporeal Perfusion Device for Small Animal Free Flaps. |
| title_sort | development of an extracorporeal perfusion device for small animal free flaps |
| url | http://europepmc.org/articles/PMC4726627?pdf=render |
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