Distribution of Genes and Microbial Taxa Related to Soil Phosphorus Cycling across Soil Depths in Subtropical Forests
Although many studies have focused on the roles of soil microbes in phosphorus (P) cycling, little is known about the distribution of microbial P cycling genes across soil depths. In this study, metagenomic sequencing was adopted to examine the differences in the abundance of genes and microbial tax...
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| Format: | Article |
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MDPI AG
2023-08-01
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| Series: | Forests |
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| Online Access: | https://www.mdpi.com/1999-4907/14/8/1665 |
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| author | Hao Lv Jie Yang Siwen Su Yue Liu Jie Feng Yuxiang Sheng Ting Wang Jinwen Pan Li Tang Liang Chen Shuai Ouyang Guangjun Wang |
| author_facet | Hao Lv Jie Yang Siwen Su Yue Liu Jie Feng Yuxiang Sheng Ting Wang Jinwen Pan Li Tang Liang Chen Shuai Ouyang Guangjun Wang |
| author_sort | Hao Lv |
| collection | DOAJ |
| description | Although many studies have focused on the roles of soil microbes in phosphorus (P) cycling, little is known about the distribution of microbial P cycling genes across soil depths. In this study, metagenomic sequencing was adopted to examine the differences in the abundance of genes and microbial taxa associated with soil P cycling between organic and mineral soil in subtropical forests. The total relative abundance of inorganic P solubilizing genes was the highest, that of P starvation response regulating genes was second, and organic P mineralizing genes was the lowest. The soil organic carbon concentration, N:P ratio, and available P concentration were higher in the organic soil than the mineral soil, resulting in abundances of organic P mineralizing genes (<i>appA</i> and <i>3-phytase</i>), and inorganic P cycling genes (<i>ppa</i>), whereas those of the inorganic P cycling genes (<i>gcd</i> and <i>pqqC</i>) and the P starvation response regulating gene (<i>phoR</i>) were higher in mineral soil. The four bacteria phyla that related to P cycling, <i>Proteobacteria</i>, <i>Actinobacteria</i>, <i>Bacteroidetes</i>, and <i>Candidatus</i>_<i>Eremiobacteraeota</i> were higher in organic soil; conversely, the three bacteria phyla (<i>Acidobacteria</i>, <i>Verrucomicrobia</i>, and <i>Chloroflexi</i>) and archaea taxa were more abundant in mineral soil. Therefore, we concluded that the distribution of genes and microbial taxa involved in soil P cycling differed among soil depths, providing a depth-resolved scale insight into the underlying mechanisms of P cycling by soil microorganisms in subtropical forests. |
| first_indexed | 2024-03-10T23:56:15Z |
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| institution | Directory Open Access Journal |
| issn | 1999-4907 |
| language | English |
| last_indexed | 2024-03-10T23:56:15Z |
| publishDate | 2023-08-01 |
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| series | Forests |
| spelling | doaj.art-704cc26ceb2e4ca3920bcf1007a23abc2023-11-19T01:10:16ZengMDPI AGForests1999-49072023-08-01148166510.3390/f14081665Distribution of Genes and Microbial Taxa Related to Soil Phosphorus Cycling across Soil Depths in Subtropical ForestsHao Lv0Jie Yang1Siwen Su2Yue Liu3Jie Feng4Yuxiang Sheng5Ting Wang6Jinwen Pan7Li Tang8Liang Chen9Shuai Ouyang10Guangjun Wang11Faculty of Life Science and Technology, Central South University of Forestry and Technology, No. 498 Southern Shaoshan Road, Changsha 410004, ChinaFaculty of Life Science and Technology, Central South University of Forestry and Technology, No. 498 Southern Shaoshan Road, Changsha 410004, ChinaFaculty of Life Science and Technology, Central South University of Forestry and Technology, No. 498 Southern Shaoshan Road, Changsha 410004, ChinaFaculty of Life Science and Technology, Central South University of Forestry and Technology, No. 498 Southern Shaoshan Road, Changsha 410004, ChinaFaculty of Life Science and Technology, Central South University of Forestry and Technology, No. 498 Southern Shaoshan Road, Changsha 410004, ChinaFaculty of Life Science and Technology, Central South University of Forestry and Technology, No. 498 Southern Shaoshan Road, Changsha 410004, ChinaFaculty of Life Science and Technology, Central South University of Forestry and Technology, No. 498 Southern Shaoshan Road, Changsha 410004, ChinaFaculty of Life Science and Technology, Central South University of Forestry and Technology, No. 498 Southern Shaoshan Road, Changsha 410004, ChinaFaculty of Agriculture and Forestry Technology, Hunan Applied Technology University, Changde 415000, ChinaFaculty of Life Science and Technology, Central South University of Forestry and Technology, No. 498 Southern Shaoshan Road, Changsha 410004, ChinaFaculty of Life Science and Technology, Central South University of Forestry and Technology, No. 498 Southern Shaoshan Road, Changsha 410004, ChinaFaculty of Life Science and Technology, Central South University of Forestry and Technology, No. 498 Southern Shaoshan Road, Changsha 410004, ChinaAlthough many studies have focused on the roles of soil microbes in phosphorus (P) cycling, little is known about the distribution of microbial P cycling genes across soil depths. In this study, metagenomic sequencing was adopted to examine the differences in the abundance of genes and microbial taxa associated with soil P cycling between organic and mineral soil in subtropical forests. The total relative abundance of inorganic P solubilizing genes was the highest, that of P starvation response regulating genes was second, and organic P mineralizing genes was the lowest. The soil organic carbon concentration, N:P ratio, and available P concentration were higher in the organic soil than the mineral soil, resulting in abundances of organic P mineralizing genes (<i>appA</i> and <i>3-phytase</i>), and inorganic P cycling genes (<i>ppa</i>), whereas those of the inorganic P cycling genes (<i>gcd</i> and <i>pqqC</i>) and the P starvation response regulating gene (<i>phoR</i>) were higher in mineral soil. The four bacteria phyla that related to P cycling, <i>Proteobacteria</i>, <i>Actinobacteria</i>, <i>Bacteroidetes</i>, and <i>Candidatus</i>_<i>Eremiobacteraeota</i> were higher in organic soil; conversely, the three bacteria phyla (<i>Acidobacteria</i>, <i>Verrucomicrobia</i>, and <i>Chloroflexi</i>) and archaea taxa were more abundant in mineral soil. Therefore, we concluded that the distribution of genes and microbial taxa involved in soil P cycling differed among soil depths, providing a depth-resolved scale insight into the underlying mechanisms of P cycling by soil microorganisms in subtropical forests.https://www.mdpi.com/1999-4907/14/8/1665phosphate solubilizing microorganismsphosphorus cycling genessoil depthsubtropical forestsmetagenomic sequencing |
| spellingShingle | Hao Lv Jie Yang Siwen Su Yue Liu Jie Feng Yuxiang Sheng Ting Wang Jinwen Pan Li Tang Liang Chen Shuai Ouyang Guangjun Wang Distribution of Genes and Microbial Taxa Related to Soil Phosphorus Cycling across Soil Depths in Subtropical Forests Forests phosphate solubilizing microorganisms phosphorus cycling genes soil depth subtropical forests metagenomic sequencing |
| title | Distribution of Genes and Microbial Taxa Related to Soil Phosphorus Cycling across Soil Depths in Subtropical Forests |
| title_full | Distribution of Genes and Microbial Taxa Related to Soil Phosphorus Cycling across Soil Depths in Subtropical Forests |
| title_fullStr | Distribution of Genes and Microbial Taxa Related to Soil Phosphorus Cycling across Soil Depths in Subtropical Forests |
| title_full_unstemmed | Distribution of Genes and Microbial Taxa Related to Soil Phosphorus Cycling across Soil Depths in Subtropical Forests |
| title_short | Distribution of Genes and Microbial Taxa Related to Soil Phosphorus Cycling across Soil Depths in Subtropical Forests |
| title_sort | distribution of genes and microbial taxa related to soil phosphorus cycling across soil depths in subtropical forests |
| topic | phosphate solubilizing microorganisms phosphorus cycling genes soil depth subtropical forests metagenomic sequencing |
| url | https://www.mdpi.com/1999-4907/14/8/1665 |
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