Effect of Chitosan Electrospun Fiber Mesh as Template on the Crystallization of Calcium Oxalate
Biominerals fulfill various physiological functions in living organisms, however, pathological mineralization can also occur generating mineral pathologies such as the formation of calcium oxalate (CaOx) calculi in the urinary tract. Inspired by the ability of living organisms to generate biogenic m...
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MDPI AG
2020-05-01
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| Series: | Crystals |
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| Online Access: | https://www.mdpi.com/2073-4352/10/6/453 |
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| author | Nicole Butto Nicole Cotrina Vera Felipe Díaz-Soler Mehrdad Yazdani-Pedram Andrónico Neira-Carrillo |
| author_facet | Nicole Butto Nicole Cotrina Vera Felipe Díaz-Soler Mehrdad Yazdani-Pedram Andrónico Neira-Carrillo |
| author_sort | Nicole Butto |
| collection | DOAJ |
| description | Biominerals fulfill various physiological functions in living organisms, however, pathological mineralization can also occur generating mineral pathologies such as the formation of calcium oxalate (CaOx) calculi in the urinary tract. Inspired by the ability of living organisms to generate biogenic minerals using biological organic matrices, and the need to understand the mechanisms of crystallization, three-dimensional fibrillary meshes based on chitosan fibers with random and controlled topology by electrospinning were manufactured. Chitosan was selected due to its active role on in vitro crystallization and its physicochemical properties, which allows the exposure of their functional chemical groups that could selectively stabilize hydrated crystalline forms of CaOx. CaOx crystals were generated on conductive tin indium oxide (ITO) glass substrates modified with electrospun chitosan fibers by using electrocrystallization (EC) technique. The chitosan fibers and the resulting CaOx crystals were analyzed by optical microscopy (OM), scanning electron microscopy (SEM), and X-ray diffraction (XRD) techniques, which demonstrated that the chemical nature and topology of the three-dimensional fibers used as organic template are key factors in the control of type, morphology, and crystallographic orientation of CaOx. |
| first_indexed | 2024-03-10T19:27:43Z |
| format | Article |
| id | doaj.art-705176aba38440b9aa3f575f0bb4fdbe |
| institution | Directory Open Access Journal |
| issn | 2073-4352 |
| language | English |
| last_indexed | 2024-03-10T19:27:43Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Crystals |
| spelling | doaj.art-705176aba38440b9aa3f575f0bb4fdbe2023-11-20T02:23:42ZengMDPI AGCrystals2073-43522020-05-0110645310.3390/cryst10060453Effect of Chitosan Electrospun Fiber Mesh as Template on the Crystallization of Calcium OxalateNicole Butto0Nicole Cotrina Vera1Felipe Díaz-Soler2Mehrdad Yazdani-Pedram3Andrónico Neira-Carrillo4Department of Biological and Animal Sciences, Faculty of Veterinary and Animal Sciences, University of Chile, Santiago, Santa Rosa 11735. La Pintana, 8820808 Santiago, ChileDepartment of Biological and Animal Sciences, Faculty of Veterinary and Animal Sciences, University of Chile, Santiago, Santa Rosa 11735. La Pintana, 8820808 Santiago, ChileDepartment of Biological and Animal Sciences, Faculty of Veterinary and Animal Sciences, University of Chile, Santiago, Santa Rosa 11735. La Pintana, 8820808 Santiago, ChileDepartment of Organic and Physical Chemistry, University of Chile. S. Olivos 1007. Independencia, 8380544 Santiago, ChileDepartment of Biological and Animal Sciences, Faculty of Veterinary and Animal Sciences, University of Chile, Santiago, Santa Rosa 11735. La Pintana, 8820808 Santiago, ChileBiominerals fulfill various physiological functions in living organisms, however, pathological mineralization can also occur generating mineral pathologies such as the formation of calcium oxalate (CaOx) calculi in the urinary tract. Inspired by the ability of living organisms to generate biogenic minerals using biological organic matrices, and the need to understand the mechanisms of crystallization, three-dimensional fibrillary meshes based on chitosan fibers with random and controlled topology by electrospinning were manufactured. Chitosan was selected due to its active role on in vitro crystallization and its physicochemical properties, which allows the exposure of their functional chemical groups that could selectively stabilize hydrated crystalline forms of CaOx. CaOx crystals were generated on conductive tin indium oxide (ITO) glass substrates modified with electrospun chitosan fibers by using electrocrystallization (EC) technique. The chitosan fibers and the resulting CaOx crystals were analyzed by optical microscopy (OM), scanning electron microscopy (SEM), and X-ray diffraction (XRD) techniques, which demonstrated that the chemical nature and topology of the three-dimensional fibers used as organic template are key factors in the control of type, morphology, and crystallographic orientation of CaOx.https://www.mdpi.com/2073-4352/10/6/453calcium oxalatechitosanelectrocrystallizationfiberselectrospinning |
| spellingShingle | Nicole Butto Nicole Cotrina Vera Felipe Díaz-Soler Mehrdad Yazdani-Pedram Andrónico Neira-Carrillo Effect of Chitosan Electrospun Fiber Mesh as Template on the Crystallization of Calcium Oxalate Crystals calcium oxalate chitosan electrocrystallization fibers electrospinning |
| title | Effect of Chitosan Electrospun Fiber Mesh as Template on the Crystallization of Calcium Oxalate |
| title_full | Effect of Chitosan Electrospun Fiber Mesh as Template on the Crystallization of Calcium Oxalate |
| title_fullStr | Effect of Chitosan Electrospun Fiber Mesh as Template on the Crystallization of Calcium Oxalate |
| title_full_unstemmed | Effect of Chitosan Electrospun Fiber Mesh as Template on the Crystallization of Calcium Oxalate |
| title_short | Effect of Chitosan Electrospun Fiber Mesh as Template on the Crystallization of Calcium Oxalate |
| title_sort | effect of chitosan electrospun fiber mesh as template on the crystallization of calcium oxalate |
| topic | calcium oxalate chitosan electrocrystallization fibers electrospinning |
| url | https://www.mdpi.com/2073-4352/10/6/453 |
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