Association of Thr307Ala and Asn680Ser of Follicle-Stimulating Hormone Receptor Gene Polymorphisms with Gonadotropin Administration during Controlled Ovarian Hyperstimulation
Objective: This study is to investigate the effect of different single-nucleotide polymorphisms of follicle-stimulating hormone receptor (FSHR) gene on gonadotropin (Gn) administration dosage during controlled ovarian hyperstimulation (COH) protocol of in vitro fertilization and embryo transfer. Met...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Health/LWW
2018-01-01
|
| Series: | Reproductive and Developmental Medicine |
| Subjects: | |
| Online Access: | http://www.repdevmed.org/article.asp?issn=2096-2924;year=2018;volume=2;issue=2;spage=81;epage=87;aulast=Meng |
| _version_ | 1811326826429022208 |
|---|---|
| author | Qing-Xia Meng Dan Song Hong Li Wei Wang Jian Ou Yong-Le Xu Ai-Yan Zheng |
| author_facet | Qing-Xia Meng Dan Song Hong Li Wei Wang Jian Ou Yong-Le Xu Ai-Yan Zheng |
| author_sort | Qing-Xia Meng |
| collection | DOAJ |
| description | Objective: This study is to investigate the effect of different single-nucleotide polymorphisms of follicle-stimulating hormone receptor (FSHR) gene on gonadotropin (Gn) administration dosage during controlled ovarian hyperstimulation (COH) protocol of in vitro fertilization and embryo transfer.
Methods: This retrospective study included 184 Chinese infertile women in Center for Reproduction and Genetics of Suzhou Municipal Hospital from June 2012 to 2014. All of the enrolled patients were homogeneous in some basal characteristics, and they all met the eligibility criteria. Blood tests were conducted on day 3 of menstrual cycle or the day of human chorionic gonadotropin administration for hormonal profile analysis and DNA extraction. DNA sequencing was performed for polymorphism analysis. The participants were classified into threonine (Thr)/Thr, Thr/alanine (Ala), and Ala/Ala groups according to genotype at position 307, and asparagine/asparagine (Asn/Asn), Asn/serine (Ser), and Ser/Ser groups according to genotype at position 680. Logistic regression and correlation analyses were performed to identify the effect of these two polymorphisms on Gn consumption.
Results: The frequency of Thr307Ala and Asn680Ser distribution was consistent with Hardy–Weinberg equilibrium (P > 0.05). No significant difference was found in age, basal hormone levels for different genotype groups. Logistic regression analysis results revealed that patients with Ser680Ser genotype have a higher risk of requiring a high dose of Gn compared with patients with Asn680Asn genotype, while polymorphism of Thr307 Ala has no such effect.
Conclusion: This study suggested that FSHR genotype Asn680Ser would be helpful in determining the dosage of Gn in COH; patients with Ser680Ser genotype may require higher dose of Gn. |
| first_indexed | 2024-04-13T14:55:20Z |
| format | Article |
| id | doaj.art-70535d31dbdd42bc9a963428046428cc |
| institution | Directory Open Access Journal |
| issn | 2096-2924 |
| language | English |
| last_indexed | 2024-04-13T14:55:20Z |
| publishDate | 2018-01-01 |
| publisher | Wolters Kluwer Health/LWW |
| record_format | Article |
| series | Reproductive and Developmental Medicine |
| spelling | doaj.art-70535d31dbdd42bc9a963428046428cc2022-12-22T02:42:27ZengWolters Kluwer Health/LWWReproductive and Developmental Medicine2096-29242018-01-0122818710.4103/2096-2924.242750Association of Thr307Ala and Asn680Ser of Follicle-Stimulating Hormone Receptor Gene Polymorphisms with Gonadotropin Administration during Controlled Ovarian HyperstimulationQing-Xia MengDan SongHong LiWei WangJian OuYong-Le XuAi-Yan ZhengObjective: This study is to investigate the effect of different single-nucleotide polymorphisms of follicle-stimulating hormone receptor (FSHR) gene on gonadotropin (Gn) administration dosage during controlled ovarian hyperstimulation (COH) protocol of in vitro fertilization and embryo transfer. Methods: This retrospective study included 184 Chinese infertile women in Center for Reproduction and Genetics of Suzhou Municipal Hospital from June 2012 to 2014. All of the enrolled patients were homogeneous in some basal characteristics, and they all met the eligibility criteria. Blood tests were conducted on day 3 of menstrual cycle or the day of human chorionic gonadotropin administration for hormonal profile analysis and DNA extraction. DNA sequencing was performed for polymorphism analysis. The participants were classified into threonine (Thr)/Thr, Thr/alanine (Ala), and Ala/Ala groups according to genotype at position 307, and asparagine/asparagine (Asn/Asn), Asn/serine (Ser), and Ser/Ser groups according to genotype at position 680. Logistic regression and correlation analyses were performed to identify the effect of these two polymorphisms on Gn consumption. Results: The frequency of Thr307Ala and Asn680Ser distribution was consistent with Hardy–Weinberg equilibrium (P > 0.05). No significant difference was found in age, basal hormone levels for different genotype groups. Logistic regression analysis results revealed that patients with Ser680Ser genotype have a higher risk of requiring a high dose of Gn compared with patients with Asn680Asn genotype, while polymorphism of Thr307 Ala has no such effect. Conclusion: This study suggested that FSHR genotype Asn680Ser would be helpful in determining the dosage of Gn in COH; patients with Ser680Ser genotype may require higher dose of Gn.http://www.repdevmed.org/article.asp?issn=2096-2924;year=2018;volume=2;issue=2;spage=81;epage=87;aulast=MengControlled Ovarian Hyperstimulation; Follicle-Stimulating Hormone Receptor; Gonadotropin; Single-Nucleotide Polymorphism |
| spellingShingle | Qing-Xia Meng Dan Song Hong Li Wei Wang Jian Ou Yong-Le Xu Ai-Yan Zheng Association of Thr307Ala and Asn680Ser of Follicle-Stimulating Hormone Receptor Gene Polymorphisms with Gonadotropin Administration during Controlled Ovarian Hyperstimulation Reproductive and Developmental Medicine Controlled Ovarian Hyperstimulation; Follicle-Stimulating Hormone Receptor; Gonadotropin; Single-Nucleotide Polymorphism |
| title | Association of Thr307Ala and Asn680Ser of Follicle-Stimulating Hormone Receptor Gene Polymorphisms with Gonadotropin Administration during Controlled Ovarian Hyperstimulation |
| title_full | Association of Thr307Ala and Asn680Ser of Follicle-Stimulating Hormone Receptor Gene Polymorphisms with Gonadotropin Administration during Controlled Ovarian Hyperstimulation |
| title_fullStr | Association of Thr307Ala and Asn680Ser of Follicle-Stimulating Hormone Receptor Gene Polymorphisms with Gonadotropin Administration during Controlled Ovarian Hyperstimulation |
| title_full_unstemmed | Association of Thr307Ala and Asn680Ser of Follicle-Stimulating Hormone Receptor Gene Polymorphisms with Gonadotropin Administration during Controlled Ovarian Hyperstimulation |
| title_short | Association of Thr307Ala and Asn680Ser of Follicle-Stimulating Hormone Receptor Gene Polymorphisms with Gonadotropin Administration during Controlled Ovarian Hyperstimulation |
| title_sort | association of thr307ala and asn680ser of follicle stimulating hormone receptor gene polymorphisms with gonadotropin administration during controlled ovarian hyperstimulation |
| topic | Controlled Ovarian Hyperstimulation; Follicle-Stimulating Hormone Receptor; Gonadotropin; Single-Nucleotide Polymorphism |
| url | http://www.repdevmed.org/article.asp?issn=2096-2924;year=2018;volume=2;issue=2;spage=81;epage=87;aulast=Meng |
| work_keys_str_mv | AT qingxiameng associationofthr307alaandasn680seroffolliclestimulatinghormonereceptorgenepolymorphismswithgonadotropinadministrationduringcontrolledovarianhyperstimulation AT dansong associationofthr307alaandasn680seroffolliclestimulatinghormonereceptorgenepolymorphismswithgonadotropinadministrationduringcontrolledovarianhyperstimulation AT hongli associationofthr307alaandasn680seroffolliclestimulatinghormonereceptorgenepolymorphismswithgonadotropinadministrationduringcontrolledovarianhyperstimulation AT weiwang associationofthr307alaandasn680seroffolliclestimulatinghormonereceptorgenepolymorphismswithgonadotropinadministrationduringcontrolledovarianhyperstimulation AT jianou associationofthr307alaandasn680seroffolliclestimulatinghormonereceptorgenepolymorphismswithgonadotropinadministrationduringcontrolledovarianhyperstimulation AT yonglexu associationofthr307alaandasn680seroffolliclestimulatinghormonereceptorgenepolymorphismswithgonadotropinadministrationduringcontrolledovarianhyperstimulation AT aiyanzheng associationofthr307alaandasn680seroffolliclestimulatinghormonereceptorgenepolymorphismswithgonadotropinadministrationduringcontrolledovarianhyperstimulation |