Lentiviral-Encoded shRNA Silencing of Proteoglycan Decorin Enhances Tendon Repair and Regeneration within a Rat Model
Injured tendons often heal with scar tissue formation, resulting in uniformly smaller collagen fibrils and poor mechanical properties. The small leucine-rich proteoglycan decorin is well known to regulate fusion of collagen fibrils. Rat patellar tendon cells were transfected with lentiviral-encoded...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2013-09-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.3727/096368912X661292 |
| _version_ | 1818342400920125440 |
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| author | Ping Lu Guo-Rong Zhang You-Zhi Cai Boon Chin Heng Hao Ren Lin-Lin Wang Junfeng Ji Xiao-Hui Zou Hong-Wei Ouyang |
| author_facet | Ping Lu Guo-Rong Zhang You-Zhi Cai Boon Chin Heng Hao Ren Lin-Lin Wang Junfeng Ji Xiao-Hui Zou Hong-Wei Ouyang |
| author_sort | Ping Lu |
| collection | DOAJ |
| description | Injured tendons often heal with scar tissue formation, resulting in uniformly smaller collagen fibrils and poor mechanical properties. The small leucine-rich proteoglycan decorin is well known to regulate fusion of collagen fibrils. Rat patellar tendon cells were transfected with lentiviral-encoded shRNA that specifically targets decorin. Silencing of decorin expression resulted in decreased cell growth. Three types of scaffold-free engineered tendons with different mix ratios of anti-decorin shRNA-treated cells to untreated cells at 1:0 (DCN), 1:1 (MIX), and 0:1 (CON) were utilized for repair of injured patellar tendons. Four weeks after implantation in situ, the MIX group manifested the best results (best coordination of histology, more mature collagen deposition, and larger collagen fibril diameter). Although the DCN group exhibited the largest collagen fibril diameter, this was associated with abnormal shape. Hence, regulation of decorin expression to an appropriate level is crucial for tendon repair with gene therapy. |
| first_indexed | 2024-12-13T16:14:06Z |
| format | Article |
| id | doaj.art-7056081f64aa447c8ef878b94a0ea79a |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-12-13T16:14:06Z |
| publishDate | 2013-09-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-7056081f64aa447c8ef878b94a0ea79a2022-12-21T23:38:52ZengSAGE PublishingCell Transplantation0963-68971555-38922013-09-012210.3727/096368912X661292Lentiviral-Encoded shRNA Silencing of Proteoglycan Decorin Enhances Tendon Repair and Regeneration within a Rat ModelPing Lu0Guo-Rong Zhang1You-Zhi Cai2Boon Chin Heng3Hao Ren4Lin-Lin Wang5Junfeng Ji6Xiao-Hui Zou7Hong-Wei Ouyang8 Zhejiang Provincial Key Laboratory of Tissue Engineering and Regenerative Medicine, Hangzhou, China Department of Basic Medicine, Changchun Medical College, Changchun, China Zhejiang Provincial Key Laboratory of Tissue Engineering and Regenerative Medicine, Hangzhou, China Department of Biosystems Science and Engineering, ETH-Zurich, Switzerland Zhejiang Provincial Key Laboratory of Tissue Engineering and Regenerative Medicine, Hangzhou, China Zhejiang Provincial Key Laboratory of Tissue Engineering and Regenerative Medicine, Hangzhou, China Central Laboratory, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China Central Laboratory, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China Department of Orthopaedic Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, ChinaInjured tendons often heal with scar tissue formation, resulting in uniformly smaller collagen fibrils and poor mechanical properties. The small leucine-rich proteoglycan decorin is well known to regulate fusion of collagen fibrils. Rat patellar tendon cells were transfected with lentiviral-encoded shRNA that specifically targets decorin. Silencing of decorin expression resulted in decreased cell growth. Three types of scaffold-free engineered tendons with different mix ratios of anti-decorin shRNA-treated cells to untreated cells at 1:0 (DCN), 1:1 (MIX), and 0:1 (CON) were utilized for repair of injured patellar tendons. Four weeks after implantation in situ, the MIX group manifested the best results (best coordination of histology, more mature collagen deposition, and larger collagen fibril diameter). Although the DCN group exhibited the largest collagen fibril diameter, this was associated with abnormal shape. Hence, regulation of decorin expression to an appropriate level is crucial for tendon repair with gene therapy.https://doi.org/10.3727/096368912X661292 |
| spellingShingle | Ping Lu Guo-Rong Zhang You-Zhi Cai Boon Chin Heng Hao Ren Lin-Lin Wang Junfeng Ji Xiao-Hui Zou Hong-Wei Ouyang Lentiviral-Encoded shRNA Silencing of Proteoglycan Decorin Enhances Tendon Repair and Regeneration within a Rat Model Cell Transplantation |
| title | Lentiviral-Encoded shRNA Silencing of Proteoglycan Decorin Enhances Tendon Repair and Regeneration within a Rat Model |
| title_full | Lentiviral-Encoded shRNA Silencing of Proteoglycan Decorin Enhances Tendon Repair and Regeneration within a Rat Model |
| title_fullStr | Lentiviral-Encoded shRNA Silencing of Proteoglycan Decorin Enhances Tendon Repair and Regeneration within a Rat Model |
| title_full_unstemmed | Lentiviral-Encoded shRNA Silencing of Proteoglycan Decorin Enhances Tendon Repair and Regeneration within a Rat Model |
| title_short | Lentiviral-Encoded shRNA Silencing of Proteoglycan Decorin Enhances Tendon Repair and Regeneration within a Rat Model |
| title_sort | lentiviral encoded shrna silencing of proteoglycan decorin enhances tendon repair and regeneration within a rat model |
| url | https://doi.org/10.3727/096368912X661292 |
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