Effects of nitrogen fertilizers on the bacterial community diversity and the weathering of purple mudstone in Southwest China
IntroductionRock weathering is crucial in the development of soil. Yet the role of bacteria in the fine particle-forming process of purple mudstone is not fully understood, especially under nitrogen fertilization.MethodsIn this study, the particles (0.25 mm to 1 mm) of purple mudstone from Penglai G...
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| Format: | Article |
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Frontiers Media S.A.
2023-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1164826/full |
| _version_ | 1797792159867338752 |
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| author | Chunpei Li Maopan Fan Xuan Wang Xuan Wang Xue Li Guang Zhao Gangcai Liu Jixia Zhao |
| author_facet | Chunpei Li Maopan Fan Xuan Wang Xuan Wang Xue Li Guang Zhao Gangcai Liu Jixia Zhao |
| author_sort | Chunpei Li |
| collection | DOAJ |
| description | IntroductionRock weathering is crucial in the development of soil. Yet the role of bacteria in the fine particle-forming process of purple mudstone is not fully understood, especially under nitrogen fertilization.MethodsIn this study, the particles (0.25 mm to 1 mm) of purple mudstone from Penglai Group (J3p) were selected as the test material. Two nitrogen fertilizers, i.e., urea (U) and ammonium bicarbonate (AB), and four application levels (0, 280, 560, and 840 N kg∙ha−1) with 18 replications were designed in an incubation experiment. The weathering indices and bacterial community structure of the purple mudstone particles were investigated after 120 days of incubation.ResultsThe results showed that the weathering indices of purple mudstone particles in the AB treatment were higher than that in the U treatment at the same fertilization levels and a reducing trend was observed with increasing nitrogen fertilizer levels under the same nitrogen fertilizer application types. The diversities of the bacterial community were extremely significantly altered by nitrogen fertilizer application (p < 0.01). The effect of the nitrogen fertilizer application level on the beta diversity of the bacterial community (R2 = 0.34) was greater than that of the nitrogen fertilizer application type (R2 = 0.20). Through stepwise regression analysis, the positive effects of nitrification of Nitrobacter (Nitrolancea) (R2 = 0.36), the Phosphorous-dissolving bacteria (Massilia) (R2 = 0.12), and N-NO3− (R2 = 0.35) on the weathering indices of J3p purple mudstone particles could be observed. Structural equation modelling indicated that nitrogen fertilizer application level affects the abundance of the dominant species at the genus level (Nitrolancea and Massilia), and key environmental factor (N-NO3−), which in turn accelerated the weathering indices (59%).Discussion and ConclusionOur findings imply that the enhancements of nitrification of Nitrobacter (Nitrolancea) and of phosphorus solubilization of Phosphorous-dissolving bacteria (Massilia) by nitrogen fertilization are the key factors affecting the weathering indices of J3p purple mudstone particles. |
| first_indexed | 2024-03-13T02:29:12Z |
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| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-13T02:29:12Z |
| publishDate | 2023-06-01 |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-7058edd041164d579a19070d4c0885912023-06-30T01:33:02ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-06-011410.3389/fmicb.2023.11648261164826Effects of nitrogen fertilizers on the bacterial community diversity and the weathering of purple mudstone in Southwest ChinaChunpei Li0Maopan Fan1Xuan Wang2Xuan Wang3Xue Li4Guang Zhao5Gangcai Liu6Jixia Zhao7College of Resources and Environment, Yunnan Agricultural University, Kunming, ChinaCollege of Resources and Environment, Yunnan Agricultural University, Kunming, ChinaKey Laboratory of Mountain Surface Processes and Ecological Regulation, Chinese Academy of Sciences, Institute of Mountain Hazards and Environment, Chinese Academy of Sciences, and Ministry of Water Conservancy, Chengdu, ChinaUniversity of the Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing, ChinaCollege of Resources and Environment, Yunnan Agricultural University, Kunming, ChinaKey Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Mountain Surface Processes and Ecological Regulation, Chinese Academy of Sciences, Institute of Mountain Hazards and Environment, Chinese Academy of Sciences, and Ministry of Water Conservancy, Chengdu, ChinaCollege of Resources and Environment, Yunnan Agricultural University, Kunming, ChinaIntroductionRock weathering is crucial in the development of soil. Yet the role of bacteria in the fine particle-forming process of purple mudstone is not fully understood, especially under nitrogen fertilization.MethodsIn this study, the particles (0.25 mm to 1 mm) of purple mudstone from Penglai Group (J3p) were selected as the test material. Two nitrogen fertilizers, i.e., urea (U) and ammonium bicarbonate (AB), and four application levels (0, 280, 560, and 840 N kg∙ha−1) with 18 replications were designed in an incubation experiment. The weathering indices and bacterial community structure of the purple mudstone particles were investigated after 120 days of incubation.ResultsThe results showed that the weathering indices of purple mudstone particles in the AB treatment were higher than that in the U treatment at the same fertilization levels and a reducing trend was observed with increasing nitrogen fertilizer levels under the same nitrogen fertilizer application types. The diversities of the bacterial community were extremely significantly altered by nitrogen fertilizer application (p < 0.01). The effect of the nitrogen fertilizer application level on the beta diversity of the bacterial community (R2 = 0.34) was greater than that of the nitrogen fertilizer application type (R2 = 0.20). Through stepwise regression analysis, the positive effects of nitrification of Nitrobacter (Nitrolancea) (R2 = 0.36), the Phosphorous-dissolving bacteria (Massilia) (R2 = 0.12), and N-NO3− (R2 = 0.35) on the weathering indices of J3p purple mudstone particles could be observed. Structural equation modelling indicated that nitrogen fertilizer application level affects the abundance of the dominant species at the genus level (Nitrolancea and Massilia), and key environmental factor (N-NO3−), which in turn accelerated the weathering indices (59%).Discussion and ConclusionOur findings imply that the enhancements of nitrification of Nitrobacter (Nitrolancea) and of phosphorus solubilization of Phosphorous-dissolving bacteria (Massilia) by nitrogen fertilization are the key factors affecting the weathering indices of J3p purple mudstone particles.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1164826/fullpurple mudstone weatheringbacterial community structureureaammonium bicarbonateweathering indices |
| spellingShingle | Chunpei Li Maopan Fan Xuan Wang Xuan Wang Xue Li Guang Zhao Gangcai Liu Jixia Zhao Effects of nitrogen fertilizers on the bacterial community diversity and the weathering of purple mudstone in Southwest China Frontiers in Microbiology purple mudstone weathering bacterial community structure urea ammonium bicarbonate weathering indices |
| title | Effects of nitrogen fertilizers on the bacterial community diversity and the weathering of purple mudstone in Southwest China |
| title_full | Effects of nitrogen fertilizers on the bacterial community diversity and the weathering of purple mudstone in Southwest China |
| title_fullStr | Effects of nitrogen fertilizers on the bacterial community diversity and the weathering of purple mudstone in Southwest China |
| title_full_unstemmed | Effects of nitrogen fertilizers on the bacterial community diversity and the weathering of purple mudstone in Southwest China |
| title_short | Effects of nitrogen fertilizers on the bacterial community diversity and the weathering of purple mudstone in Southwest China |
| title_sort | effects of nitrogen fertilizers on the bacterial community diversity and the weathering of purple mudstone in southwest china |
| topic | purple mudstone weathering bacterial community structure urea ammonium bicarbonate weathering indices |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1164826/full |
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