SOX2 promoter hypermethylation in non-smoking Taiwanese adults residing in air pollution areas

Abstract Background Both SOX2 promoter methylation and air pollution have been associated with lung cancer risk. However, little has been done to assess SOX2 promoter methylation in individuals living in air pollution areas. The aim of this study was to investigate SOX2 promoter methylation in non-s...

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Main Authors: Disline Manli Tantoh, Ming-Fang Wu, Chien-Chang Ho, Chia-Chi Lung, Kuan-Jung Lee, Oswald Ndi Nfor, Yi-Chia Liaw, Shu-Yi Hsu, Pei-Hsin Chen, Chin Lin, Hou-Wei Chu, Yi-Ching Liaw, Yung-Po Liaw
Format: Article
Language:English
Published: BMC 2019-03-01
Series:Clinical Epigenetics
Subjects:
Online Access:http://link.springer.com/article/10.1186/s13148-019-0647-8
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author Disline Manli Tantoh
Ming-Fang Wu
Chien-Chang Ho
Chia-Chi Lung
Kuan-Jung Lee
Oswald Ndi Nfor
Yi-Chia Liaw
Shu-Yi Hsu
Pei-Hsin Chen
Chin Lin
Hou-Wei Chu
Yi-Ching Liaw
Yung-Po Liaw
author_facet Disline Manli Tantoh
Ming-Fang Wu
Chien-Chang Ho
Chia-Chi Lung
Kuan-Jung Lee
Oswald Ndi Nfor
Yi-Chia Liaw
Shu-Yi Hsu
Pei-Hsin Chen
Chin Lin
Hou-Wei Chu
Yi-Ching Liaw
Yung-Po Liaw
author_sort Disline Manli Tantoh
collection DOAJ
description Abstract Background Both SOX2 promoter methylation and air pollution have been associated with lung cancer risk. However, little has been done to assess SOX2 promoter methylation in individuals living in air pollution areas. The aim of this study was to investigate SOX2 promoter methylation in non-smoking Taiwanese adults living in areas with different levels of air pollution especially particulate matter with diameter < 2.5 μm (PM2.5). Methods A total of 1142 individuals aged 30–70 years were recruited. Data on SOX2 methylation, residence, age, and exposure to second-hand smoke (SHS) among others were extracted from the Taiwan Biobank dataset (2008–2015). After excluding former and current smokers, alongside those with incomplete information, a total of 461 non-smokers comprising 176 men and 285 women were included in the study. Participants’ residences were grouped under northern and central/southern areas because air pollution (PM2.5) is lower in northern compared to central and southern areas. Results The methylation levels in men (0.16310 ± 0.01230) and women (0.15740 ± 0.01240) were significantly different (P < .0001). In both sexes, the SOX2 promoter region was shown to be significantly hypermethylated in central and southern areas compared with the northern areas. The regression coefficient (β) was 0.00331 (P = 0.0257) in men and 0.00514 (P < .0001) in women. Conclusion SOX2 was significantly hypermethylated in both men and women residing in central and southern areas. The consistency in the results for both sexes shows that SOX2 promoter methylation could serve as a potential biomarker for industrial air pollution exposure. Moreover, it might reflect predisposition to cancer. Hence, healthy non-smokers at precancerous stages who have not been clinically diagnosed could be identified.
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spelling doaj.art-705935dbfb3642e1b7f6618af9aed8922022-12-21T22:32:16ZengBMCClinical Epigenetics1868-70751868-70832019-03-011111710.1186/s13148-019-0647-8SOX2 promoter hypermethylation in non-smoking Taiwanese adults residing in air pollution areasDisline Manli Tantoh0Ming-Fang Wu1Chien-Chang Ho2Chia-Chi Lung3Kuan-Jung Lee4Oswald Ndi Nfor5Yi-Chia Liaw6Shu-Yi Hsu7Pei-Hsin Chen8Chin Lin9Hou-Wei Chu10Yi-Ching Liaw11Yung-Po Liaw12Department of Public Health and Institute of Public Health, Chung Shan Medical UniversityDepartment of Public Health and Institute of Public Health, Chung Shan Medical UniversityDepartment of Physical Education, Fu-Jen Catholic UniversityDepartment of Public Health and Institute of Public Health, Chung Shan Medical UniversityDepartment of Public Health and Institute of Public Health, Chung Shan Medical UniversityDepartment of Public Health and Institute of Public Health, Chung Shan Medical UniversityDepartment of Medical Education, Taipei Veterans General HospitalDepartment of Public Health and Institute of Public Health, Chung Shan Medical UniversityDepartment of Public Health and Institute of Public Health, Chung Shan Medical UniversityGraduate Institute of Life Sciences, National Defense Medical CenterInstitute of Biomedical Sciences, Academia SinicaInstitute of Clinical Medicine, National Yang-Ming UniversityDepartment of Public Health and Institute of Public Health, Chung Shan Medical UniversityAbstract Background Both SOX2 promoter methylation and air pollution have been associated with lung cancer risk. However, little has been done to assess SOX2 promoter methylation in individuals living in air pollution areas. The aim of this study was to investigate SOX2 promoter methylation in non-smoking Taiwanese adults living in areas with different levels of air pollution especially particulate matter with diameter < 2.5 μm (PM2.5). Methods A total of 1142 individuals aged 30–70 years were recruited. Data on SOX2 methylation, residence, age, and exposure to second-hand smoke (SHS) among others were extracted from the Taiwan Biobank dataset (2008–2015). After excluding former and current smokers, alongside those with incomplete information, a total of 461 non-smokers comprising 176 men and 285 women were included in the study. Participants’ residences were grouped under northern and central/southern areas because air pollution (PM2.5) is lower in northern compared to central and southern areas. Results The methylation levels in men (0.16310 ± 0.01230) and women (0.15740 ± 0.01240) were significantly different (P < .0001). In both sexes, the SOX2 promoter region was shown to be significantly hypermethylated in central and southern areas compared with the northern areas. The regression coefficient (β) was 0.00331 (P = 0.0257) in men and 0.00514 (P < .0001) in women. Conclusion SOX2 was significantly hypermethylated in both men and women residing in central and southern areas. The consistency in the results for both sexes shows that SOX2 promoter methylation could serve as a potential biomarker for industrial air pollution exposure. Moreover, it might reflect predisposition to cancer. Hence, healthy non-smokers at precancerous stages who have not been clinically diagnosed could be identified.http://link.springer.com/article/10.1186/s13148-019-0647-8SOX2HypermethylationNon-smokingAir pollutionBiomarkerTaiwan Biobank
spellingShingle Disline Manli Tantoh
Ming-Fang Wu
Chien-Chang Ho
Chia-Chi Lung
Kuan-Jung Lee
Oswald Ndi Nfor
Yi-Chia Liaw
Shu-Yi Hsu
Pei-Hsin Chen
Chin Lin
Hou-Wei Chu
Yi-Ching Liaw
Yung-Po Liaw
SOX2 promoter hypermethylation in non-smoking Taiwanese adults residing in air pollution areas
Clinical Epigenetics
SOX2
Hypermethylation
Non-smoking
Air pollution
Biomarker
Taiwan Biobank
title SOX2 promoter hypermethylation in non-smoking Taiwanese adults residing in air pollution areas
title_full SOX2 promoter hypermethylation in non-smoking Taiwanese adults residing in air pollution areas
title_fullStr SOX2 promoter hypermethylation in non-smoking Taiwanese adults residing in air pollution areas
title_full_unstemmed SOX2 promoter hypermethylation in non-smoking Taiwanese adults residing in air pollution areas
title_short SOX2 promoter hypermethylation in non-smoking Taiwanese adults residing in air pollution areas
title_sort sox2 promoter hypermethylation in non smoking taiwanese adults residing in air pollution areas
topic SOX2
Hypermethylation
Non-smoking
Air pollution
Biomarker
Taiwan Biobank
url http://link.springer.com/article/10.1186/s13148-019-0647-8
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