Preparative isolation of cerebrosides (galactosyl and glucosyl ceramide)
An improved method for isolating cerebrosides from natural sources is described. The method is particularly suited to large scale work and can be adapted to the isolation of sphingolipids that are less polar than the gangliosides. It is based on the use of sodium sulfate to absorb the water from chl...
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| Format: | Article |
| Language: | English |
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Elsevier
1976-05-01
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| Series: | Journal of Lipid Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520369893 |
| _version_ | 1819055451292041216 |
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| author | N S Radin |
| author_facet | N S Radin |
| author_sort | N S Radin |
| collection | DOAJ |
| description | An improved method for isolating cerebrosides from natural sources is described. The method is particularly suited to large scale work and can be adapted to the isolation of sphingolipids that are less polar than the gangliosides. It is based on the use of sodium sulfate to absorb the water from chloroform-methanol tissue extracts, the use of triiodide to cleave the ether linkage of plasmalogens, and the use of alkaline methanolysis to cleave the ester linkages of the glycerolipids. The final separation of the lipids is done with a silica gel column. |
| first_indexed | 2024-12-21T13:07:43Z |
| format | Article |
| id | doaj.art-705c67dc0eec437ca8abeb1e0195aa3b |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-21T13:07:43Z |
| publishDate | 1976-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-705c67dc0eec437ca8abeb1e0195aa3b2022-12-21T19:02:58ZengElsevierJournal of Lipid Research0022-22751976-05-01173290293Preparative isolation of cerebrosides (galactosyl and glucosyl ceramide)N S Radin0Mental Health Research Institute and Department of Biologzcal Chemistry, University of Michigan, Ann Arbor, Michigan 48109An improved method for isolating cerebrosides from natural sources is described. The method is particularly suited to large scale work and can be adapted to the isolation of sphingolipids that are less polar than the gangliosides. It is based on the use of sodium sulfate to absorb the water from chloroform-methanol tissue extracts, the use of triiodide to cleave the ether linkage of plasmalogens, and the use of alkaline methanolysis to cleave the ester linkages of the glycerolipids. The final separation of the lipids is done with a silica gel column.http://www.sciencedirect.com/science/article/pii/S0022227520369893glucocerebrosidegalactocerebroside |
| spellingShingle | N S Radin Preparative isolation of cerebrosides (galactosyl and glucosyl ceramide) Journal of Lipid Research glucocerebroside galactocerebroside |
| title | Preparative isolation of cerebrosides (galactosyl and glucosyl ceramide) |
| title_full | Preparative isolation of cerebrosides (galactosyl and glucosyl ceramide) |
| title_fullStr | Preparative isolation of cerebrosides (galactosyl and glucosyl ceramide) |
| title_full_unstemmed | Preparative isolation of cerebrosides (galactosyl and glucosyl ceramide) |
| title_short | Preparative isolation of cerebrosides (galactosyl and glucosyl ceramide) |
| title_sort | preparative isolation of cerebrosides galactosyl and glucosyl ceramide |
| topic | glucocerebroside galactocerebroside |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520369893 |
| work_keys_str_mv | AT nsradin preparativeisolationofcerebrosidesgalactosylandglucosylceramide |