Transrectal Absorber Guide Raster‐Scanning Optoacoustic Mesoscopy for Label‐Free In Vivo Assessment of Colitis
Abstract Optoacoustic imaging (OAI) enables microscale imaging of endogenous chromophores such as hemoglobin at significantly higher penetration depths compared to other optical imaging technologies. Raster‐scanning optoacoustic mesoscopy (RSOM) has recently been shown to identify superficial microv...
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| Format: | Article |
| Language: | English |
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Wiley
2023-06-01
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| Series: | Advanced Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/advs.202300564 |
| _version_ | 1827918386709921792 |
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| author | Adrian Buehler Emma Brown Lars‐Philip Paulus Markus Eckstein Oana‐Maria Thoma Mariam‐Eleni Oraiopoulou Ulrich Rother André Hoerning Arndt Hartmann Markus F. Neurath Joachim Woelfle Oliver Friedrich Maximilian J. Waldner Ferdinand Knieling Sarah E. Bohndiek Adrian P. Regensburger |
| author_facet | Adrian Buehler Emma Brown Lars‐Philip Paulus Markus Eckstein Oana‐Maria Thoma Mariam‐Eleni Oraiopoulou Ulrich Rother André Hoerning Arndt Hartmann Markus F. Neurath Joachim Woelfle Oliver Friedrich Maximilian J. Waldner Ferdinand Knieling Sarah E. Bohndiek Adrian P. Regensburger |
| author_sort | Adrian Buehler |
| collection | DOAJ |
| description | Abstract Optoacoustic imaging (OAI) enables microscale imaging of endogenous chromophores such as hemoglobin at significantly higher penetration depths compared to other optical imaging technologies. Raster‐scanning optoacoustic mesoscopy (RSOM) has recently been shown to identify superficial microvascular changes associated with human skin pathologies. In animal models, the imaging depth afforded by RSOM can enable entirely new capabilities for noninvasive imaging of vascular structures in the gastrointestinal tract, but exact localization of intra‐abdominal organs is still elusive. Herein the development and application of a novel transrectal absorber guide for RSOM (TAG‐RSOM) is presented to enable accurate transabdominal localization and assessment of colonic vascular networks in vivo. The potential of TAG‐RSOM is demonstrated through application during mild and severe acute colitis in mice. TAG‐RSOM enables visualization of transmural vascular networks, with changes in colon wall thickness, blood volume, and OAI signal intensities corresponding to colitis‐associated inflammatory changes. These findings suggest TAG‐RSOM can provide a novel monitoring tool in preclinical IBD models, refining animal procedures and underlines the capabilities of such technologies to address inflammatory bowel diseases in humans. |
| first_indexed | 2024-03-13T03:41:33Z |
| format | Article |
| id | doaj.art-705cb5c5b0764e01b319addb400d04d3 |
| institution | Directory Open Access Journal |
| issn | 2198-3844 |
| language | English |
| last_indexed | 2024-03-13T03:41:33Z |
| publishDate | 2023-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj.art-705cb5c5b0764e01b319addb400d04d32023-06-23T07:34:34ZengWileyAdvanced Science2198-38442023-06-011018n/an/a10.1002/advs.202300564Transrectal Absorber Guide Raster‐Scanning Optoacoustic Mesoscopy for Label‐Free In Vivo Assessment of ColitisAdrian Buehler0Emma Brown1Lars‐Philip Paulus2Markus Eckstein3Oana‐Maria Thoma4Mariam‐Eleni Oraiopoulou5Ulrich Rother6André Hoerning7Arndt Hartmann8Markus F. Neurath9Joachim Woelfle10Oliver Friedrich11Maximilian J. Waldner12Ferdinand Knieling13Sarah E. Bohndiek14Adrian P. Regensburger15Department of Pediatrics and Adolescent Medicine University Hospital Erlangen Friedrich‐Alexander‐Universität (FAU) Erlangen‐Nürnberg 91054 Erlangen GermanyDepartment of Physics and Cancer Research UK Cambridge Institute University of Cambridge Cambridge CB2 0RE UKDepartment of Pediatrics and Adolescent Medicine University Hospital Erlangen Friedrich‐Alexander‐Universität (FAU) Erlangen‐Nürnberg 91054 Erlangen GermanyInstitute of Pathology Friedrich‐Alexander‐Universität (FAU) Erlangen‐Nürnberg 91054 Erlangen GermanyDepartment of Medicine 1 University Hospital Erlangen Friedrich‐Alexander‐Universität (FAU) Erlangen‐Nürnberg 91052 Erlangen GermanyDepartment of Physics and Cancer Research UK Cambridge Institute University of Cambridge Cambridge CB2 0RE UKDepartment of Vascular Surgery University Hospital Erlangen Friedrich‐Alexander‐Universität (FAU) Erlangen‐Nürnberg 91054 Erlangen GermanyDepartment of Pediatrics and Adolescent Medicine University Hospital Erlangen Friedrich‐Alexander‐Universität (FAU) Erlangen‐Nürnberg 91054 Erlangen GermanyInstitute of Pathology Friedrich‐Alexander‐Universität (FAU) Erlangen‐Nürnberg 91054 Erlangen GermanyDepartment of Medicine 1 University Hospital Erlangen Friedrich‐Alexander‐Universität (FAU) Erlangen‐Nürnberg 91052 Erlangen GermanyDepartment of Pediatrics and Adolescent Medicine University Hospital Erlangen Friedrich‐Alexander‐Universität (FAU) Erlangen‐Nürnberg 91054 Erlangen GermanyInstitute of Medical Biotechnology Department of Chemical and Biological Engineering Friedrich‐Alexander‐Universität (FAU) Erlangen‐Nürnberg 91052 Erlangen GermanyDepartment of Medicine 1 University Hospital Erlangen Friedrich‐Alexander‐Universität (FAU) Erlangen‐Nürnberg 91052 Erlangen GermanyDepartment of Pediatrics and Adolescent Medicine University Hospital Erlangen Friedrich‐Alexander‐Universität (FAU) Erlangen‐Nürnberg 91054 Erlangen GermanyDepartment of Physics and Cancer Research UK Cambridge Institute University of Cambridge Cambridge CB2 0RE UKDepartment of Pediatrics and Adolescent Medicine University Hospital Erlangen Friedrich‐Alexander‐Universität (FAU) Erlangen‐Nürnberg 91054 Erlangen GermanyAbstract Optoacoustic imaging (OAI) enables microscale imaging of endogenous chromophores such as hemoglobin at significantly higher penetration depths compared to other optical imaging technologies. Raster‐scanning optoacoustic mesoscopy (RSOM) has recently been shown to identify superficial microvascular changes associated with human skin pathologies. In animal models, the imaging depth afforded by RSOM can enable entirely new capabilities for noninvasive imaging of vascular structures in the gastrointestinal tract, but exact localization of intra‐abdominal organs is still elusive. Herein the development and application of a novel transrectal absorber guide for RSOM (TAG‐RSOM) is presented to enable accurate transabdominal localization and assessment of colonic vascular networks in vivo. The potential of TAG‐RSOM is demonstrated through application during mild and severe acute colitis in mice. TAG‐RSOM enables visualization of transmural vascular networks, with changes in colon wall thickness, blood volume, and OAI signal intensities corresponding to colitis‐associated inflammatory changes. These findings suggest TAG‐RSOM can provide a novel monitoring tool in preclinical IBD models, refining animal procedures and underlines the capabilities of such technologies to address inflammatory bowel diseases in humans.https://doi.org/10.1002/advs.202300564dextran sodium sulfate induced colitisinflammatory bowel diseasemurine acute colitisoptoacoustic imagingphotoacoustic imagingrasterscanning optoacoustic mesoscopy |
| spellingShingle | Adrian Buehler Emma Brown Lars‐Philip Paulus Markus Eckstein Oana‐Maria Thoma Mariam‐Eleni Oraiopoulou Ulrich Rother André Hoerning Arndt Hartmann Markus F. Neurath Joachim Woelfle Oliver Friedrich Maximilian J. Waldner Ferdinand Knieling Sarah E. Bohndiek Adrian P. Regensburger Transrectal Absorber Guide Raster‐Scanning Optoacoustic Mesoscopy for Label‐Free In Vivo Assessment of Colitis Advanced Science dextran sodium sulfate induced colitis inflammatory bowel disease murine acute colitis optoacoustic imaging photoacoustic imaging rasterscanning optoacoustic mesoscopy |
| title | Transrectal Absorber Guide Raster‐Scanning Optoacoustic Mesoscopy for Label‐Free In Vivo Assessment of Colitis |
| title_full | Transrectal Absorber Guide Raster‐Scanning Optoacoustic Mesoscopy for Label‐Free In Vivo Assessment of Colitis |
| title_fullStr | Transrectal Absorber Guide Raster‐Scanning Optoacoustic Mesoscopy for Label‐Free In Vivo Assessment of Colitis |
| title_full_unstemmed | Transrectal Absorber Guide Raster‐Scanning Optoacoustic Mesoscopy for Label‐Free In Vivo Assessment of Colitis |
| title_short | Transrectal Absorber Guide Raster‐Scanning Optoacoustic Mesoscopy for Label‐Free In Vivo Assessment of Colitis |
| title_sort | transrectal absorber guide raster scanning optoacoustic mesoscopy for label free in vivo assessment of colitis |
| topic | dextran sodium sulfate induced colitis inflammatory bowel disease murine acute colitis optoacoustic imaging photoacoustic imaging rasterscanning optoacoustic mesoscopy |
| url | https://doi.org/10.1002/advs.202300564 |
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