Hydroethanolic extract and fractions of Pericopsis laxiflora stem bark protect against liver injury in Rat
Pericopsis laxiflora is a medicinal plant that is ubiquitous, especially in the tropical and savannah regions. It can be used in the treatment of hemorrhoids, rheumatism, abdominal pain treatment, diarrhoea, dysentery, fever, skin diseases, and jaundice. The present study investigated the protective...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-06-01
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| Series: | Scientific African |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468227624001315 |
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| author | Frederick Sarfo-Antwi Christopher Larbie Benjamin Obukowho Emikpe Regina Appiah–Opong |
| author_facet | Frederick Sarfo-Antwi Christopher Larbie Benjamin Obukowho Emikpe Regina Appiah–Opong |
| author_sort | Frederick Sarfo-Antwi |
| collection | DOAJ |
| description | Pericopsis laxiflora is a medicinal plant that is ubiquitous, especially in the tropical and savannah regions. It can be used in the treatment of hemorrhoids, rheumatism, abdominal pain treatment, diarrhoea, dysentery, fever, skin diseases, and jaundice. The present study investigated the protective effect of 50% hydroethanolic extract and fractions of Pericopsis laxiflora stem bark in lead-induced hepatotoxicity in rats. The development of liver injury has become a global issue and their treatment and management mostly result in more economic problems, especially in the African population.The stem bark of P. laxiflora was processed and 50 % hydroethanolic extract (HSE) was prepared. HSE was subjected to sequential fractionation with organic solvents: chloroform (CFHSE), ethyl acetate (EFHSE), and methanol (MFHSE), to obtain respective fractions. The residue was designated as aqueous fraction (AFHSE). Hepatoprotective activities of HSE and fractions (at 250 mg/kg) and Silymarin were assessed using the lead (as lead acetate; 50 mg/kg, i.p.). Body and organ weight changes, biochemical, hematological profile, oxidative stress parameters, and pro-inflammatory cytokines (TNF-α, TGF-β, NF кβ, and COX-2) in liver homogenate were measured. A histopathological examination of the liver was performed. Treatment with HSE and fractions resulted in a significant increase in relative organ weights, GSH, CAT, SOD, and levels and a significant decrease in MDA and MPO levels against Pb, as well as the restoration of liver biomarkers and pro-inflammatory cytokines (TNF-α, TGF-β, NF кβ, and COX-2) to near-normal levels. Biochemical data were corroborated by histological observations, with MFHSE recording the highest percentage of liver protection. The present investigation suggests that HSE possesses remarkable hepatoprotective properties and this can be attributed to the inhibitory effect on oxidative stress and suppression of pro-inflammatory cytokines. |
| first_indexed | 2024-04-24T16:25:49Z |
| format | Article |
| id | doaj.art-705ecacc7eef43328a208fecd8364c87 |
| institution | Directory Open Access Journal |
| issn | 2468-2276 |
| language | English |
| last_indexed | 2024-04-24T16:25:49Z |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Scientific African |
| spelling | doaj.art-705ecacc7eef43328a208fecd8364c872024-03-31T04:37:52ZengElsevierScientific African2468-22762024-06-0124e02186Hydroethanolic extract and fractions of Pericopsis laxiflora stem bark protect against liver injury in RatFrederick Sarfo-Antwi0Christopher Larbie1Benjamin Obukowho Emikpe2Regina Appiah–Opong3Department of Biochemistry and Molecular Medicine, School of Medicine, University for Development Studies, Tamale, GhanaDepartment of Biochemistry and Biotechnology, College of Science, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana; Corresponding author:Department of Pathobiology, School of Veterinary Medicine, Kwame Nkrumah University of Science and Technology, Kumasi, GhanaDepartment of Clinical Pathology, Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of Ghana, Accra, GhanaPericopsis laxiflora is a medicinal plant that is ubiquitous, especially in the tropical and savannah regions. It can be used in the treatment of hemorrhoids, rheumatism, abdominal pain treatment, diarrhoea, dysentery, fever, skin diseases, and jaundice. The present study investigated the protective effect of 50% hydroethanolic extract and fractions of Pericopsis laxiflora stem bark in lead-induced hepatotoxicity in rats. The development of liver injury has become a global issue and their treatment and management mostly result in more economic problems, especially in the African population.The stem bark of P. laxiflora was processed and 50 % hydroethanolic extract (HSE) was prepared. HSE was subjected to sequential fractionation with organic solvents: chloroform (CFHSE), ethyl acetate (EFHSE), and methanol (MFHSE), to obtain respective fractions. The residue was designated as aqueous fraction (AFHSE). Hepatoprotective activities of HSE and fractions (at 250 mg/kg) and Silymarin were assessed using the lead (as lead acetate; 50 mg/kg, i.p.). Body and organ weight changes, biochemical, hematological profile, oxidative stress parameters, and pro-inflammatory cytokines (TNF-α, TGF-β, NF кβ, and COX-2) in liver homogenate were measured. A histopathological examination of the liver was performed. Treatment with HSE and fractions resulted in a significant increase in relative organ weights, GSH, CAT, SOD, and levels and a significant decrease in MDA and MPO levels against Pb, as well as the restoration of liver biomarkers and pro-inflammatory cytokines (TNF-α, TGF-β, NF кβ, and COX-2) to near-normal levels. Biochemical data were corroborated by histological observations, with MFHSE recording the highest percentage of liver protection. The present investigation suggests that HSE possesses remarkable hepatoprotective properties and this can be attributed to the inhibitory effect on oxidative stress and suppression of pro-inflammatory cytokines.http://www.sciencedirect.com/science/article/pii/S2468227624001315Body weightLiver biomarkersMedicinal plantsPro-inflammatory cytokinesOxidative stress |
| spellingShingle | Frederick Sarfo-Antwi Christopher Larbie Benjamin Obukowho Emikpe Regina Appiah–Opong Hydroethanolic extract and fractions of Pericopsis laxiflora stem bark protect against liver injury in Rat Scientific African Body weight Liver biomarkers Medicinal plants Pro-inflammatory cytokines Oxidative stress |
| title | Hydroethanolic extract and fractions of Pericopsis laxiflora stem bark protect against liver injury in Rat |
| title_full | Hydroethanolic extract and fractions of Pericopsis laxiflora stem bark protect against liver injury in Rat |
| title_fullStr | Hydroethanolic extract and fractions of Pericopsis laxiflora stem bark protect against liver injury in Rat |
| title_full_unstemmed | Hydroethanolic extract and fractions of Pericopsis laxiflora stem bark protect against liver injury in Rat |
| title_short | Hydroethanolic extract and fractions of Pericopsis laxiflora stem bark protect against liver injury in Rat |
| title_sort | hydroethanolic extract and fractions of pericopsis laxiflora stem bark protect against liver injury in rat |
| topic | Body weight Liver biomarkers Medicinal plants Pro-inflammatory cytokines Oxidative stress |
| url | http://www.sciencedirect.com/science/article/pii/S2468227624001315 |
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