Cell-Free Circulating Mitochondrial DNA: A Potential Blood-Based Marker for Atrial Fibrillation
Atrial fibrillation (AF), the most common, progressive tachyarrhythmia is associated with serious complications, such as stroke and heart failure. Early recognition of AF, essential to prevent disease progression and therapy failure, is hampered by the lack of accurate diagnostic serum biomarkers to...
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MDPI AG
2020-05-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/9/5/1159 |
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| author | Marit Wiersma Denise M.S. van Marion Emma J. Bouman Jin Li Deli Zhang Kennedy S. Ramos Eva A.H. Lanters Natasja M.S. de Groot Bianca J.J.M. Brundel |
| author_facet | Marit Wiersma Denise M.S. van Marion Emma J. Bouman Jin Li Deli Zhang Kennedy S. Ramos Eva A.H. Lanters Natasja M.S. de Groot Bianca J.J.M. Brundel |
| author_sort | Marit Wiersma |
| collection | DOAJ |
| description | Atrial fibrillation (AF), the most common, progressive tachyarrhythmia is associated with serious complications, such as stroke and heart failure. Early recognition of AF, essential to prevent disease progression and therapy failure, is hampered by the lack of accurate diagnostic serum biomarkers to identify the AF stage. As we previously showed mitochondrial dysfunction to drive experimental and human AF, we evaluated whether cell-free circulating mitochondrial DNA (cfc-mtDNA) represents a potential serum marker. Therefore, the levels of two mtDNA genes, COX3 and ND1, were measured in 84 control patients (C), 59 patients undergoing cardiac surgery without a history of AF (SR), 100 paroxysmal (PAF), 116 persistent (PeAF), and 20 longstanding-persistent (LS-PeAF) AF patients undergoing either cardiac surgery or AF treatment (electrical cardioversion or pulmonary vein isolation). Cfc-mtDNA levels were significantly increased in PAF patients undergoing AF treatment, especially in males and patients with AF recurrence after AF treatment. In PeAF and LS-PeAF, cfc-mtDNA levels gradually decreased. Importantly, cfc-mtDNA in serum may originate from cardiomyocytes, as in vitro tachypaced cardiomyocytes release mtDNA in the medium. The findings suggest that cfc-mtDNA is associated with AF stage, especially in males, and with patients at risk for AF recurrence after treatment. |
| first_indexed | 2024-03-10T19:58:26Z |
| format | Article |
| id | doaj.art-705ee3830a114361b2fb4b87db0c01c9 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T19:58:26Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-705ee3830a114361b2fb4b87db0c01c92023-11-19T23:46:24ZengMDPI AGCells2073-44092020-05-0195115910.3390/cells9051159Cell-Free Circulating Mitochondrial DNA: A Potential Blood-Based Marker for Atrial FibrillationMarit Wiersma0Denise M.S. van Marion1Emma J. Bouman2Jin Li3Deli Zhang4Kennedy S. Ramos5Eva A.H. Lanters6Natasja M.S. de Groot7Bianca J.J.M. Brundel8Department of Physiology, Amsterdam UMC, Vrije Universiteit Amsterdam, Amsterdam Cardiovascular Sciences, 1081 HV Amsterdam, The NetherlandsDepartment of Physiology, Amsterdam UMC, Vrije Universiteit Amsterdam, Amsterdam Cardiovascular Sciences, 1081 HV Amsterdam, The NetherlandsDepartment of Physiology, Amsterdam UMC, Vrije Universiteit Amsterdam, Amsterdam Cardiovascular Sciences, 1081 HV Amsterdam, The NetherlandsDepartment of Physiology, Amsterdam UMC, Vrije Universiteit Amsterdam, Amsterdam Cardiovascular Sciences, 1081 HV Amsterdam, The NetherlandsDepartment of Physiology, Amsterdam UMC, Vrije Universiteit Amsterdam, Amsterdam Cardiovascular Sciences, 1081 HV Amsterdam, The NetherlandsDepartment of Physiology, Amsterdam UMC, Vrije Universiteit Amsterdam, Amsterdam Cardiovascular Sciences, 1081 HV Amsterdam, The NetherlandsDepartment of Cardiology, Erasmus Medical Center, 3015 DG Rotterdam, The NetherlandsDepartment of Cardiology, Erasmus Medical Center, 3015 DG Rotterdam, The NetherlandsDepartment of Physiology, Amsterdam UMC, Vrije Universiteit Amsterdam, Amsterdam Cardiovascular Sciences, 1081 HV Amsterdam, The NetherlandsAtrial fibrillation (AF), the most common, progressive tachyarrhythmia is associated with serious complications, such as stroke and heart failure. Early recognition of AF, essential to prevent disease progression and therapy failure, is hampered by the lack of accurate diagnostic serum biomarkers to identify the AF stage. As we previously showed mitochondrial dysfunction to drive experimental and human AF, we evaluated whether cell-free circulating mitochondrial DNA (cfc-mtDNA) represents a potential serum marker. Therefore, the levels of two mtDNA genes, COX3 and ND1, were measured in 84 control patients (C), 59 patients undergoing cardiac surgery without a history of AF (SR), 100 paroxysmal (PAF), 116 persistent (PeAF), and 20 longstanding-persistent (LS-PeAF) AF patients undergoing either cardiac surgery or AF treatment (electrical cardioversion or pulmonary vein isolation). Cfc-mtDNA levels were significantly increased in PAF patients undergoing AF treatment, especially in males and patients with AF recurrence after AF treatment. In PeAF and LS-PeAF, cfc-mtDNA levels gradually decreased. Importantly, cfc-mtDNA in serum may originate from cardiomyocytes, as in vitro tachypaced cardiomyocytes release mtDNA in the medium. The findings suggest that cfc-mtDNA is associated with AF stage, especially in males, and with patients at risk for AF recurrence after treatment.https://www.mdpi.com/2073-4409/9/5/1159atrial fibrillationbiomarkermitochondrial DNAserum |
| spellingShingle | Marit Wiersma Denise M.S. van Marion Emma J. Bouman Jin Li Deli Zhang Kennedy S. Ramos Eva A.H. Lanters Natasja M.S. de Groot Bianca J.J.M. Brundel Cell-Free Circulating Mitochondrial DNA: A Potential Blood-Based Marker for Atrial Fibrillation Cells atrial fibrillation biomarker mitochondrial DNA serum |
| title | Cell-Free Circulating Mitochondrial DNA: A Potential Blood-Based Marker for Atrial Fibrillation |
| title_full | Cell-Free Circulating Mitochondrial DNA: A Potential Blood-Based Marker for Atrial Fibrillation |
| title_fullStr | Cell-Free Circulating Mitochondrial DNA: A Potential Blood-Based Marker for Atrial Fibrillation |
| title_full_unstemmed | Cell-Free Circulating Mitochondrial DNA: A Potential Blood-Based Marker for Atrial Fibrillation |
| title_short | Cell-Free Circulating Mitochondrial DNA: A Potential Blood-Based Marker for Atrial Fibrillation |
| title_sort | cell free circulating mitochondrial dna a potential blood based marker for atrial fibrillation |
| topic | atrial fibrillation biomarker mitochondrial DNA serum |
| url | https://www.mdpi.com/2073-4409/9/5/1159 |
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