Biochemical and histological characterization of <i>Agave fourcroydes</i> Lem. plants <i>in vitro</i> acclimatized
Henequen (Agave fourcroydes Lem.) is a representative crop of the province of Matanzas, Cuba, which has been exploited extensively for the quality of its fibers. The present work aimed to characterize from the biochemical and histological point of view henequen plants cv. 'Sac Ki' in vitro...
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| Format: | Article |
| Language: | English |
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Universidad Central Marta Abreu de Las Villas
2016-10-01
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| Series: | Biotecnología Vegetal |
| Online Access: | https://revista.ibp.co.cu/index.php/BV/article/view/529 |
| _version_ | 1818257788823928832 |
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| author | Yunel Pérez Hernández Maryla Sosa del Castillo Leticia Fuentes Alfonso Yasmary Rubio Fontanill Aymara L Valdivia Ávila Jovana Pérez Ramos |
| author_facet | Yunel Pérez Hernández Maryla Sosa del Castillo Leticia Fuentes Alfonso Yasmary Rubio Fontanill Aymara L Valdivia Ávila Jovana Pérez Ramos |
| author_sort | Yunel Pérez Hernández |
| collection | DOAJ |
| description | Henequen (Agave fourcroydes Lem.) is a representative crop of the province of Matanzas, Cuba, which has been exploited extensively for the quality of its fibers. The present work aimed to characterize from the biochemical and histological point of view henequen plants cv. 'Sac Ki' in vitro acclimatized. The biochemical indicators analyzed were concentration of soluble phenols, total soluble carbohydrates, reducing sugars, soluble proteins and total chlorophylls, as well as the relative contents of flavonoids, terpins and anthocyanins. In addition, a histological analysis of the epidermis was performed and the results were compared with plants of one month in rooting culture medium. In vitro acclimatized plants were subsequently transferred to the ex vitro conditions. The contents of total soluble carbohydrates, reducing sugars and chlorophylls decreased, whereas the levels of soluble phenols, proteins and secondary metabolites were higher in relation to in vitro plants rooted for 30 days. The epidermis of the in vitro acclimatized plants showed a greater development of the stomatal apparatus that could positively affect the ex vitro acclimatization, where a survival rate of 87% and a reduction of the acclimatization time were obtained.
Keywords: chlorophyll, henequen, phenol, reducing sugar |
| first_indexed | 2024-12-12T17:49:13Z |
| format | Article |
| id | doaj.art-70678c188d1947068ede060d54265aac |
| institution | Directory Open Access Journal |
| issn | 1609-1841 2074-8647 |
| language | English |
| last_indexed | 2024-12-12T17:49:13Z |
| publishDate | 2016-10-01 |
| publisher | Universidad Central Marta Abreu de Las Villas |
| record_format | Article |
| series | Biotecnología Vegetal |
| spelling | doaj.art-70678c188d1947068ede060d54265aac2022-12-22T00:16:51ZengUniversidad Central Marta Abreu de Las VillasBiotecnología Vegetal1609-18412074-86472016-10-01164489Biochemical and histological characterization of <i>Agave fourcroydes</i> Lem. plants <i>in vitro</i> acclimatizedYunel Pérez HernándezMaryla Sosa del CastilloLeticia Fuentes AlfonsoYasmary Rubio FontanillAymara L Valdivia ÁvilaJovana Pérez RamosHenequen (Agave fourcroydes Lem.) is a representative crop of the province of Matanzas, Cuba, which has been exploited extensively for the quality of its fibers. The present work aimed to characterize from the biochemical and histological point of view henequen plants cv. 'Sac Ki' in vitro acclimatized. The biochemical indicators analyzed were concentration of soluble phenols, total soluble carbohydrates, reducing sugars, soluble proteins and total chlorophylls, as well as the relative contents of flavonoids, terpins and anthocyanins. In addition, a histological analysis of the epidermis was performed and the results were compared with plants of one month in rooting culture medium. In vitro acclimatized plants were subsequently transferred to the ex vitro conditions. The contents of total soluble carbohydrates, reducing sugars and chlorophylls decreased, whereas the levels of soluble phenols, proteins and secondary metabolites were higher in relation to in vitro plants rooted for 30 days. The epidermis of the in vitro acclimatized plants showed a greater development of the stomatal apparatus that could positively affect the ex vitro acclimatization, where a survival rate of 87% and a reduction of the acclimatization time were obtained. Keywords: chlorophyll, henequen, phenol, reducing sugarhttps://revista.ibp.co.cu/index.php/BV/article/view/529 |
| spellingShingle | Yunel Pérez Hernández Maryla Sosa del Castillo Leticia Fuentes Alfonso Yasmary Rubio Fontanill Aymara L Valdivia Ávila Jovana Pérez Ramos Biochemical and histological characterization of <i>Agave fourcroydes</i> Lem. plants <i>in vitro</i> acclimatized Biotecnología Vegetal |
| title | Biochemical and histological characterization of <i>Agave fourcroydes</i> Lem. plants <i>in vitro</i> acclimatized |
| title_full | Biochemical and histological characterization of <i>Agave fourcroydes</i> Lem. plants <i>in vitro</i> acclimatized |
| title_fullStr | Biochemical and histological characterization of <i>Agave fourcroydes</i> Lem. plants <i>in vitro</i> acclimatized |
| title_full_unstemmed | Biochemical and histological characterization of <i>Agave fourcroydes</i> Lem. plants <i>in vitro</i> acclimatized |
| title_short | Biochemical and histological characterization of <i>Agave fourcroydes</i> Lem. plants <i>in vitro</i> acclimatized |
| title_sort | biochemical and histological characterization of i agave fourcroydes i lem plants i in vitro i acclimatized |
| url | https://revista.ibp.co.cu/index.php/BV/article/view/529 |
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