Colorimetric and electrochemical dual-signal detection of uracil-DNA glycosylase using functionalized pure DNA hydrogel on paper-based analytical devices
The development of simple and accurate detection of uracil-DNA glycosylase (UDG) is of great significance for early clinical diagnosis and biomedical research. Here, we on the first effort introduced the uracil bases into the rolling circle amplification (RCA) reaction to produce the functionalized...
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KeAi Communications Co., Ltd.
2023-09-01
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| Series: | Advanced Agrochem |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2773237123000473 |
| _version_ | 1797235468343967744 |
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| author | Wei Xue Pan Jia Yunping Wu Pu Wang Jiarong Shi Yangyang Chang Meng Liu |
| author_facet | Wei Xue Pan Jia Yunping Wu Pu Wang Jiarong Shi Yangyang Chang Meng Liu |
| author_sort | Wei Xue |
| collection | DOAJ |
| description | The development of simple and accurate detection of uracil-DNA glycosylase (UDG) is of great significance for early clinical diagnosis and biomedical research. Here, we on the first effort introduced the uracil bases into the rolling circle amplification (RCA) reaction to produce the functionalized pure DNA hydrogel (PDH) for UDG detection. During RCA process, methylene blue (MB) molecules as the indicators were encapsulated into PDH. The addition of UDG can remove the uracil bases of PDH to generate abasic sites, which are further cleaved with the assistance of apurinic/apyrimidinic endonuclease (APE), thus resulting in the dissociation of PDH to release blue MB. By combining with the paper analytical devices as the signal readout platform, a colorimetric and electrochemical dual-signal biosensor was constructed for convenient and accurate detection of UDG. The proposed MB@PDH-based dual-signal sensing system exhibited good selectivity and high sensitivity with a detection limit of 6.4 × 10−4 U/mL (electrochemical method). It was also demonstrated that this sensing system showed excellent performance in UDG inhibitor screening, thus providing great potential in UDG-related disease diagnosis and drug discovery. |
| first_indexed | 2024-04-24T16:48:26Z |
| format | Article |
| id | doaj.art-70683f2b5fc345dc87b2138d5c61fd5a |
| institution | Directory Open Access Journal |
| issn | 2773-2371 |
| language | English |
| last_indexed | 2024-04-24T16:48:26Z |
| publishDate | 2023-09-01 |
| publisher | KeAi Communications Co., Ltd. |
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| series | Advanced Agrochem |
| spelling | doaj.art-70683f2b5fc345dc87b2138d5c61fd5a2024-03-29T05:51:41ZengKeAi Communications Co., Ltd.Advanced Agrochem2773-23712023-09-0123269275Colorimetric and electrochemical dual-signal detection of uracil-DNA glycosylase using functionalized pure DNA hydrogel on paper-based analytical devicesWei Xue0Pan Jia1Yunping Wu2Pu Wang3Jiarong Shi4Yangyang Chang5Meng Liu6School of Environmental Science and Technology, Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), Dalian University of Technology, Dalian, 116024, China; Dalian POCT Laboratory, Dalian, 116024, ChinaSchool of Environmental Science and Technology, Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), Dalian University of Technology, Dalian, 116024, China; Dalian POCT Laboratory, Dalian, 116024, ChinaSchool of Environmental Science and Technology, Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), Dalian University of Technology, Dalian, 116024, China; Dalian POCT Laboratory, Dalian, 116024, ChinaSchool of Environmental Science and Technology, Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), Dalian University of Technology, Dalian, 116024, China; Dalian POCT Laboratory, Dalian, 116024, ChinaSchool of Environmental Science and Technology, Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), Dalian University of Technology, Dalian, 116024, China; Dalian POCT Laboratory, Dalian, 116024, ChinaSchool of Environmental Science and Technology, Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), Dalian University of Technology, Dalian, 116024, China; Dalian POCT Laboratory, Dalian, 116024, China; Corresponding author. Dalian POCT Laboratory, Dalian, 116024, China.School of Environmental Science and Technology, Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), Dalian University of Technology, Dalian, 116024, China; Dalian POCT Laboratory, Dalian, 116024, China; Corresponding author. Dalian POCT Laboratory, Dalian, 116024, China.The development of simple and accurate detection of uracil-DNA glycosylase (UDG) is of great significance for early clinical diagnosis and biomedical research. Here, we on the first effort introduced the uracil bases into the rolling circle amplification (RCA) reaction to produce the functionalized pure DNA hydrogel (PDH) for UDG detection. During RCA process, methylene blue (MB) molecules as the indicators were encapsulated into PDH. The addition of UDG can remove the uracil bases of PDH to generate abasic sites, which are further cleaved with the assistance of apurinic/apyrimidinic endonuclease (APE), thus resulting in the dissociation of PDH to release blue MB. By combining with the paper analytical devices as the signal readout platform, a colorimetric and electrochemical dual-signal biosensor was constructed for convenient and accurate detection of UDG. The proposed MB@PDH-based dual-signal sensing system exhibited good selectivity and high sensitivity with a detection limit of 6.4 × 10−4 U/mL (electrochemical method). It was also demonstrated that this sensing system showed excellent performance in UDG inhibitor screening, thus providing great potential in UDG-related disease diagnosis and drug discovery.http://www.sciencedirect.com/science/article/pii/S2773237123000473Pure DNA hydrogelUracil-DNA glycosylaseDual-signal detectionRolling circle amplificationPaper-based analytical device |
| spellingShingle | Wei Xue Pan Jia Yunping Wu Pu Wang Jiarong Shi Yangyang Chang Meng Liu Colorimetric and electrochemical dual-signal detection of uracil-DNA glycosylase using functionalized pure DNA hydrogel on paper-based analytical devices Advanced Agrochem Pure DNA hydrogel Uracil-DNA glycosylase Dual-signal detection Rolling circle amplification Paper-based analytical device |
| title | Colorimetric and electrochemical dual-signal detection of uracil-DNA glycosylase using functionalized pure DNA hydrogel on paper-based analytical devices |
| title_full | Colorimetric and electrochemical dual-signal detection of uracil-DNA glycosylase using functionalized pure DNA hydrogel on paper-based analytical devices |
| title_fullStr | Colorimetric and electrochemical dual-signal detection of uracil-DNA glycosylase using functionalized pure DNA hydrogel on paper-based analytical devices |
| title_full_unstemmed | Colorimetric and electrochemical dual-signal detection of uracil-DNA glycosylase using functionalized pure DNA hydrogel on paper-based analytical devices |
| title_short | Colorimetric and electrochemical dual-signal detection of uracil-DNA glycosylase using functionalized pure DNA hydrogel on paper-based analytical devices |
| title_sort | colorimetric and electrochemical dual signal detection of uracil dna glycosylase using functionalized pure dna hydrogel on paper based analytical devices |
| topic | Pure DNA hydrogel Uracil-DNA glycosylase Dual-signal detection Rolling circle amplification Paper-based analytical device |
| url | http://www.sciencedirect.com/science/article/pii/S2773237123000473 |
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