Does Osmotic Stress Affect Natural Product Expression in Fungi?
The discovery of new natural products from fungi isolated from the marine environment has increased dramatically over the last few decades, leading to the identification of over 1000 new metabolites. However, most of the reported marine-derived species appear to be terrestrial in origin yet at the s...
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-08-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/15/8/254 |
| _version_ | 1811303997011656704 |
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| author | David Overy Hebelin Correa Catherine Roullier Wei-Chiung Chi Ka-Lai Pang Mostafa Rateb Rainer Ebel Zhuo Shang Rob Capon Gerald Bills Russell Kerr |
| author_facet | David Overy Hebelin Correa Catherine Roullier Wei-Chiung Chi Ka-Lai Pang Mostafa Rateb Rainer Ebel Zhuo Shang Rob Capon Gerald Bills Russell Kerr |
| author_sort | David Overy |
| collection | DOAJ |
| description | The discovery of new natural products from fungi isolated from the marine environment has increased dramatically over the last few decades, leading to the identification of over 1000 new metabolites. However, most of the reported marine-derived species appear to be terrestrial in origin yet at the same time, facultatively halo- or osmotolerant. An unanswered question regarding the apparent chemical productivity of marine-derived fungi is whether the common practice of fermenting strains in seawater contributes to enhanced secondary metabolism? To answer this question, a terrestrial isolate of Aspergillus aculeatus was fermented in osmotic and saline stress conditions in parallel across multiple sites. The ex-type strain of A. aculeatus was obtained from three different culture collections. Site-to-site variations in metabolite expression were observed, suggesting that subculturing of the same strain and subtle variations in experimental protocols can have pronounced effects upon metabolite expression. Replicated experiments at individual sites indicated that secondary metabolite production was divergent between osmotic and saline treatments. Titers of some metabolites increased or decreased in response to increasing osmolite (salt or glycerol) concentrations. Furthermore, in some cases, the expression of some secondary metabolites in relation to osmotic and saline stress was attributed to specific sources of the ex-type strains. |
| first_indexed | 2024-04-13T07:58:08Z |
| format | Article |
| id | doaj.art-706a6db7f4f241c8a033bad8b4859227 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-13T07:58:08Z |
| publishDate | 2017-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-706a6db7f4f241c8a033bad8b48592272022-12-22T02:55:21ZengMDPI AGMarine Drugs1660-33972017-08-0115825410.3390/md15080254md15080254Does Osmotic Stress Affect Natural Product Expression in Fungi?David Overy0Hebelin Correa1Catherine Roullier2Wei-Chiung Chi3Ka-Lai Pang4Mostafa Rateb5Rainer Ebel6Zhuo Shang7Rob Capon8Gerald Bills9Russell Kerr10Department of Chemistry, University of Prince Edward Island, 550 University Avenue, Charlottetown, PE C1A 4P3, CanadaDepartment of Chemistry, University of Prince Edward Island, 550 University Avenue, Charlottetown, PE C1A 4P3, CanadaMer Molécules Santé—EA 2160, UFR des Sciences Pharmaceutiques et Biologiques, Université de Nantes, 9 Rue Bias, 44035 Nantes, FranceDepartment of Food Science, National Quemoy University, Kinmen County 89250, TaiwanInstitute of Marine Biology and Centre of Excellence for the Oceans, National Taiwan Ocean University, 2 Pei-Ning Road, Keelung 20224, TaiwanMarine Biodiscovery Centre, Department of Chemistry, University of Aberdeen, Meston Walk, Aberdeen AB24 3UE, Scotland, UKMarine Biodiscovery Centre, Department of Chemistry, University of Aberdeen, Meston Walk, Aberdeen AB24 3UE, Scotland, UKDivision of Chemistry and Structural Biology, Institute for Molecular Bioscience, The University of Queensland, 306 Carmody Road, St. Lucia QLD 4072, AustraliaDivision of Chemistry and Structural Biology, Institute for Molecular Bioscience, The University of Queensland, 306 Carmody Road, St. Lucia QLD 4072, AustraliaTexas Therapeutics Institute, The Brown Foundation Institute of Molecular Medicine, University of Texas Health Science Center, 1881 East Rd., Houston, TX 77054, USADepartment of Chemistry, University of Prince Edward Island, 550 University Avenue, Charlottetown, PE C1A 4P3, CanadaThe discovery of new natural products from fungi isolated from the marine environment has increased dramatically over the last few decades, leading to the identification of over 1000 new metabolites. However, most of the reported marine-derived species appear to be terrestrial in origin yet at the same time, facultatively halo- or osmotolerant. An unanswered question regarding the apparent chemical productivity of marine-derived fungi is whether the common practice of fermenting strains in seawater contributes to enhanced secondary metabolism? To answer this question, a terrestrial isolate of Aspergillus aculeatus was fermented in osmotic and saline stress conditions in parallel across multiple sites. The ex-type strain of A. aculeatus was obtained from three different culture collections. Site-to-site variations in metabolite expression were observed, suggesting that subculturing of the same strain and subtle variations in experimental protocols can have pronounced effects upon metabolite expression. Replicated experiments at individual sites indicated that secondary metabolite production was divergent between osmotic and saline treatments. Titers of some metabolites increased or decreased in response to increasing osmolite (salt or glycerol) concentrations. Furthermore, in some cases, the expression of some secondary metabolites in relation to osmotic and saline stress was attributed to specific sources of the ex-type strains.https://www.mdpi.com/1660-3397/15/8/254fungimetabolite expressionLC-MSmetabolomeosmotic stress |
| spellingShingle | David Overy Hebelin Correa Catherine Roullier Wei-Chiung Chi Ka-Lai Pang Mostafa Rateb Rainer Ebel Zhuo Shang Rob Capon Gerald Bills Russell Kerr Does Osmotic Stress Affect Natural Product Expression in Fungi? Marine Drugs fungi metabolite expression LC-MS metabolome osmotic stress |
| title | Does Osmotic Stress Affect Natural Product Expression in Fungi? |
| title_full | Does Osmotic Stress Affect Natural Product Expression in Fungi? |
| title_fullStr | Does Osmotic Stress Affect Natural Product Expression in Fungi? |
| title_full_unstemmed | Does Osmotic Stress Affect Natural Product Expression in Fungi? |
| title_short | Does Osmotic Stress Affect Natural Product Expression in Fungi? |
| title_sort | does osmotic stress affect natural product expression in fungi |
| topic | fungi metabolite expression LC-MS metabolome osmotic stress |
| url | https://www.mdpi.com/1660-3397/15/8/254 |
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