Sophoricoside attenuates neuronal injury and altered cognitive function by regulating the LTR-4/NF-κB/PI3K signalling pathway in anaesthetic-exposed neonatal rats
Introduction This study examined the protective effects of sophoricoside on neuronal injury and cognitive dysfunction in anaesthetic-exposed neonatal rats. Material and methods Neuronal injury was induced in rat pups by exposure to isoflurane (0.75%) with 30% oxygen for 6 h on P7. The protective ef...
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| Format: | Article |
| Language: | English |
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Termedia Publishing House
2020-03-01
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| Series: | Archives of Medical Science |
| Subjects: | |
| Online Access: | https://www.archivesofmedicalscience.com/Sophoricoside-attenuates-neuronal-injury-and-altered-cognitive-function-by-regulating,116657,0,2.html |
| _version_ | 1827287687664500736 |
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| author | Lihua Yang Yucan Xu Wei Zhang |
| author_facet | Lihua Yang Yucan Xu Wei Zhang |
| author_sort | Lihua Yang |
| collection | DOAJ |
| description | Introduction
This study examined the protective effects of sophoricoside on neuronal injury and cognitive dysfunction in anaesthetic-exposed neonatal rats.
Material and methods
Neuronal injury was induced in rat pups by exposure to isoflurane (0.75%) with 30% oxygen for 6 h on P7. The protective effects of sophoricoside were evaluated by assessing cognitive function using the neurological score and Morris water maze. Neuronal apoptosis was assessed in hippocampus tissue using a TUNEL assay. The cytokine and macrophage inflammatory protein levels were assessed by ELISA. Western blot assays and RT-PCR were performed to assess the expression of NF-B, TLR-4, Akt, and PI3K proteins in neuronal tissues. Immunohistochemical and histopathological changes were observed in the brain tissues of isoflurane-induced neuronal injury rats.
Results
The sophoricoside treatment improved cognitive and neuronal function in rats exposed to isoflurane. Cytokine and MIP levels in the brain tissues of isoflurane-exposed rats decreased. However, sophoricoside treatment attenuated the expression of TLR-4, PI3K, and Akt protein in the brain tissues of isoflurane-exposed rats. The histopathology improved in the sophoricoside-treated isoflurane-exposed rats.
Conclusions
Sophoricoside treatment protects against neuronal injury and reduced cognitive function in isoflurane-induced neuronal injury rats by regulating TLR-4 signalling. |
| first_indexed | 2024-04-24T11:16:07Z |
| format | Article |
| id | doaj.art-706e870ac28644b9ab85ba132421172b |
| institution | Directory Open Access Journal |
| issn | 1734-1922 1896-9151 |
| language | English |
| last_indexed | 2024-04-24T11:16:07Z |
| publishDate | 2020-03-01 |
| publisher | Termedia Publishing House |
| record_format | Article |
| series | Archives of Medical Science |
| spelling | doaj.art-706e870ac28644b9ab85ba132421172b2024-04-11T08:11:16ZengTermedia Publishing HouseArchives of Medical Science1734-19221896-91512020-03-0120124825410.5114/aoms.2020.93638116657Sophoricoside attenuates neuronal injury and altered cognitive function by regulating the LTR-4/NF-κB/PI3K signalling pathway in anaesthetic-exposed neonatal ratsLihua Yang0Yucan Xu1Wei Zhang2Department of Anesthesiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, ChinaDepartment of Anesthesiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, ChinaDepartment of Anesthesiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, ChinaIntroduction This study examined the protective effects of sophoricoside on neuronal injury and cognitive dysfunction in anaesthetic-exposed neonatal rats. Material and methods Neuronal injury was induced in rat pups by exposure to isoflurane (0.75%) with 30% oxygen for 6 h on P7. The protective effects of sophoricoside were evaluated by assessing cognitive function using the neurological score and Morris water maze. Neuronal apoptosis was assessed in hippocampus tissue using a TUNEL assay. The cytokine and macrophage inflammatory protein levels were assessed by ELISA. Western blot assays and RT-PCR were performed to assess the expression of NF-B, TLR-4, Akt, and PI3K proteins in neuronal tissues. Immunohistochemical and histopathological changes were observed in the brain tissues of isoflurane-induced neuronal injury rats. Results The sophoricoside treatment improved cognitive and neuronal function in rats exposed to isoflurane. Cytokine and MIP levels in the brain tissues of isoflurane-exposed rats decreased. However, sophoricoside treatment attenuated the expression of TLR-4, PI3K, and Akt protein in the brain tissues of isoflurane-exposed rats. The histopathology improved in the sophoricoside-treated isoflurane-exposed rats. Conclusions Sophoricoside treatment protects against neuronal injury and reduced cognitive function in isoflurane-induced neuronal injury rats by regulating TLR-4 signalling.https://www.archivesofmedicalscience.com/Sophoricoside-attenuates-neuronal-injury-and-altered-cognitive-function-by-regulating,116657,0,2.htmlsophoricosideisofluranecognitive functionneuronal injurytlr-4 |
| spellingShingle | Lihua Yang Yucan Xu Wei Zhang Sophoricoside attenuates neuronal injury and altered cognitive function by regulating the LTR-4/NF-κB/PI3K signalling pathway in anaesthetic-exposed neonatal rats Archives of Medical Science sophoricoside isoflurane cognitive function neuronal injury tlr-4 |
| title | Sophoricoside attenuates neuronal injury and altered cognitive function by regulating the LTR-4/NF-κB/PI3K signalling pathway in anaesthetic-exposed neonatal rats |
| title_full | Sophoricoside attenuates neuronal injury and altered cognitive function by regulating the LTR-4/NF-κB/PI3K signalling pathway in anaesthetic-exposed neonatal rats |
| title_fullStr | Sophoricoside attenuates neuronal injury and altered cognitive function by regulating the LTR-4/NF-κB/PI3K signalling pathway in anaesthetic-exposed neonatal rats |
| title_full_unstemmed | Sophoricoside attenuates neuronal injury and altered cognitive function by regulating the LTR-4/NF-κB/PI3K signalling pathway in anaesthetic-exposed neonatal rats |
| title_short | Sophoricoside attenuates neuronal injury and altered cognitive function by regulating the LTR-4/NF-κB/PI3K signalling pathway in anaesthetic-exposed neonatal rats |
| title_sort | sophoricoside attenuates neuronal injury and altered cognitive function by regulating the ltr 4 nf κb pi3k signalling pathway in anaesthetic exposed neonatal rats |
| topic | sophoricoside isoflurane cognitive function neuronal injury tlr-4 |
| url | https://www.archivesofmedicalscience.com/Sophoricoside-attenuates-neuronal-injury-and-altered-cognitive-function-by-regulating,116657,0,2.html |
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