SPATS1 (spermatogenesis-associated, serine-rich 1) is not essential for spermatogenesis and fertility in mouse.

SPATS1 (spermatogenesis-associated, serine-rich 1) is an evolutionarily conserved, testis-specific protein that is differentially expressed during rat male meiotic prophase. Some reports have suggested a link between SPATS1 underexpression/mutation and human pathologies such as male infertility and...

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Main Authors: Carlos A Capoano, Luis Adrián Ortiz-Laquintana, Rosana Rodríguez-Casuriaga, Geraldine Schlapp, María Noel Meikle, Ana Paula Mulet, Martina Crispo, Ricardo Benavente, Adriana Geisinger
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2021-01-01
Series:PLoS ONE
Online Access:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0251028&type=printable
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author Carlos A Capoano
Luis Adrián Ortiz-Laquintana
Rosana Rodríguez-Casuriaga
Geraldine Schlapp
María Noel Meikle
Ana Paula Mulet
Martina Crispo
Ricardo Benavente
Adriana Geisinger
author_facet Carlos A Capoano
Luis Adrián Ortiz-Laquintana
Rosana Rodríguez-Casuriaga
Geraldine Schlapp
María Noel Meikle
Ana Paula Mulet
Martina Crispo
Ricardo Benavente
Adriana Geisinger
author_sort Carlos A Capoano
collection DOAJ
description SPATS1 (spermatogenesis-associated, serine-rich 1) is an evolutionarily conserved, testis-specific protein that is differentially expressed during rat male meiotic prophase. Some reports have suggested a link between SPATS1 underexpression/mutation and human pathologies such as male infertility and testicular cancer. Given the absence of functional studies, we generated a Spats1 loss-of-function mouse model using CRISPR/Cas9 technology. The phenotypic analysis showed no overt phenotype in Spats1-/- mice, with both males and females being fertile. Flow cytometry and histological analyses did not show differences in the testicular content and histology between WT and knockout mice. Moreover, no significant differences in sperm concentration, motility, and morphology, were observed between WT and KO mice. These results were obtained both for young adults and for aged animals. Besides, although an involvement of SPATS1 in the Wnt signaling pathway has been suggested, we did not detect changes in the expression levels of typical Wnt pathway-target genes in mutant individuals. Thus, albeit Spats1 alteration might be a risk factor for male testicular health, we hereby show that this gene is not individually essential for male fertility and spermatogenesis in mouse.
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spelling doaj.art-706f18dbaffc4fac8bbc7081c49f30392025-03-03T05:34:37ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-01165e025102810.1371/journal.pone.0251028SPATS1 (spermatogenesis-associated, serine-rich 1) is not essential for spermatogenesis and fertility in mouse.Carlos A CapoanoLuis Adrián Ortiz-LaquintanaRosana Rodríguez-CasuriagaGeraldine SchlappMaría Noel MeikleAna Paula MuletMartina CrispoRicardo BenaventeAdriana GeisingerSPATS1 (spermatogenesis-associated, serine-rich 1) is an evolutionarily conserved, testis-specific protein that is differentially expressed during rat male meiotic prophase. Some reports have suggested a link between SPATS1 underexpression/mutation and human pathologies such as male infertility and testicular cancer. Given the absence of functional studies, we generated a Spats1 loss-of-function mouse model using CRISPR/Cas9 technology. The phenotypic analysis showed no overt phenotype in Spats1-/- mice, with both males and females being fertile. Flow cytometry and histological analyses did not show differences in the testicular content and histology between WT and knockout mice. Moreover, no significant differences in sperm concentration, motility, and morphology, were observed between WT and KO mice. These results were obtained both for young adults and for aged animals. Besides, although an involvement of SPATS1 in the Wnt signaling pathway has been suggested, we did not detect changes in the expression levels of typical Wnt pathway-target genes in mutant individuals. Thus, albeit Spats1 alteration might be a risk factor for male testicular health, we hereby show that this gene is not individually essential for male fertility and spermatogenesis in mouse.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0251028&type=printable
spellingShingle Carlos A Capoano
Luis Adrián Ortiz-Laquintana
Rosana Rodríguez-Casuriaga
Geraldine Schlapp
María Noel Meikle
Ana Paula Mulet
Martina Crispo
Ricardo Benavente
Adriana Geisinger
SPATS1 (spermatogenesis-associated, serine-rich 1) is not essential for spermatogenesis and fertility in mouse.
PLoS ONE
title SPATS1 (spermatogenesis-associated, serine-rich 1) is not essential for spermatogenesis and fertility in mouse.
title_full SPATS1 (spermatogenesis-associated, serine-rich 1) is not essential for spermatogenesis and fertility in mouse.
title_fullStr SPATS1 (spermatogenesis-associated, serine-rich 1) is not essential for spermatogenesis and fertility in mouse.
title_full_unstemmed SPATS1 (spermatogenesis-associated, serine-rich 1) is not essential for spermatogenesis and fertility in mouse.
title_short SPATS1 (spermatogenesis-associated, serine-rich 1) is not essential for spermatogenesis and fertility in mouse.
title_sort spats1 spermatogenesis associated serine rich 1 is not essential for spermatogenesis and fertility in mouse
url https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0251028&type=printable
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