BIRC5 promotes cancer progression and predicts prognosis in laryngeal squamous cell carcinoma
Background Laryngeal squamous cell carcinoma (LSCC) remains one of the most common respiratory tumors worldwide. Baculoviral inhibitor of apoptosis repeat containing 5 (BIRC5) is a member of the inhibitor-of-apoptosis protein family. BIRC5 plays an important role in various types of cell proliferati...
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| Format: | Article |
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PeerJ Inc.
2022-02-01
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| Online Access: | https://peerj.com/articles/12871.pdf |
| _version_ | 1797419224608538624 |
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| author | Nan Wang Xuanyu Huang Jinsheng Cheng |
| author_facet | Nan Wang Xuanyu Huang Jinsheng Cheng |
| author_sort | Nan Wang |
| collection | DOAJ |
| description | Background Laryngeal squamous cell carcinoma (LSCC) remains one of the most common respiratory tumors worldwide. Baculoviral inhibitor of apoptosis repeat containing 5 (BIRC5) is a member of the inhibitor-of-apoptosis protein family. BIRC5 plays an important role in various types of cell proliferation, differentiation, migration and invasion. However, the specific role of BIRC5 in LSCC remains unclear. Methods To provide a prognostic biomarker for LSCC, we screened the prognostic genes of LSCC via bioinformatics. PPI network and KEGG pathways were used to select hub genes. Clinical prognoses were performed using a Kaplan–Meier plotter and Cox proportional-hazard analysis. BIRC5 expression in LSCC tissues and cell lines were detected by RT-PCR, Western blot and Immunohistochemistry (IHC). Cell proliferation, cell cycle and cell apoptosis were detected with Cell Counting Kit-8 (CCK-8) and Flow Cytometry assay, respectively. Results Here, BIRC5 was strongly correlated with higher tumor grade and differentiation. BIRC5 was highly expressed in LSCC tissues when compared with normal tissues and increased expression of BIRC5 was associated with overall survival in LSCC patients. The suppression of BIRC5 induced cell apoptosis and cell cycle arrest, thereby inhibiting the proliferation of LSCC cells. The survival analysis confirmed that higher level of BIRC5 expression predicted poor prognosis of LSCC patients. BIRC5 may act as an oncogene of LSCC development and was suggested as a promising prognostic biomarker for LSCC. |
| first_indexed | 2024-03-09T06:45:19Z |
| format | Article |
| id | doaj.art-706fd9abcc5a454ebba872fb608ef394 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:45:19Z |
| publishDate | 2022-02-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-706fd9abcc5a454ebba872fb608ef3942023-12-03T10:38:00ZengPeerJ Inc.PeerJ2167-83592022-02-0110e1287110.7717/peerj.12871BIRC5 promotes cancer progression and predicts prognosis in laryngeal squamous cell carcinomaNan Wang0Xuanyu Huang1Jinsheng Cheng2Guangdong Provincial Key Laboratory of Conservation and Precision Utilization of Characteristic Agricultural Resources in Mountains Areas, School of Life Science of Jiaying University, Meizhou, ChinaGuangdong Provincial Key Laboratory of Conservation and Precision Utilization of Characteristic Agricultural Resources in Mountains Areas, School of Life Science of Jiaying University, Meizhou, ChinaHenry-Fork School of Food Sciences, Shaoguan University, Shaoguan, ChinaBackground Laryngeal squamous cell carcinoma (LSCC) remains one of the most common respiratory tumors worldwide. Baculoviral inhibitor of apoptosis repeat containing 5 (BIRC5) is a member of the inhibitor-of-apoptosis protein family. BIRC5 plays an important role in various types of cell proliferation, differentiation, migration and invasion. However, the specific role of BIRC5 in LSCC remains unclear. Methods To provide a prognostic biomarker for LSCC, we screened the prognostic genes of LSCC via bioinformatics. PPI network and KEGG pathways were used to select hub genes. Clinical prognoses were performed using a Kaplan–Meier plotter and Cox proportional-hazard analysis. BIRC5 expression in LSCC tissues and cell lines were detected by RT-PCR, Western blot and Immunohistochemistry (IHC). Cell proliferation, cell cycle and cell apoptosis were detected with Cell Counting Kit-8 (CCK-8) and Flow Cytometry assay, respectively. Results Here, BIRC5 was strongly correlated with higher tumor grade and differentiation. BIRC5 was highly expressed in LSCC tissues when compared with normal tissues and increased expression of BIRC5 was associated with overall survival in LSCC patients. The suppression of BIRC5 induced cell apoptosis and cell cycle arrest, thereby inhibiting the proliferation of LSCC cells. The survival analysis confirmed that higher level of BIRC5 expression predicted poor prognosis of LSCC patients. BIRC5 may act as an oncogene of LSCC development and was suggested as a promising prognostic biomarker for LSCC.https://peerj.com/articles/12871.pdfLaryngeal squamous cell carcinomaBioinformaticsBIRC5PrognosisCell apoptosis |
| spellingShingle | Nan Wang Xuanyu Huang Jinsheng Cheng BIRC5 promotes cancer progression and predicts prognosis in laryngeal squamous cell carcinoma PeerJ Laryngeal squamous cell carcinoma Bioinformatics BIRC5 Prognosis Cell apoptosis |
| title | BIRC5 promotes cancer progression and predicts prognosis in laryngeal squamous cell carcinoma |
| title_full | BIRC5 promotes cancer progression and predicts prognosis in laryngeal squamous cell carcinoma |
| title_fullStr | BIRC5 promotes cancer progression and predicts prognosis in laryngeal squamous cell carcinoma |
| title_full_unstemmed | BIRC5 promotes cancer progression and predicts prognosis in laryngeal squamous cell carcinoma |
| title_short | BIRC5 promotes cancer progression and predicts prognosis in laryngeal squamous cell carcinoma |
| title_sort | birc5 promotes cancer progression and predicts prognosis in laryngeal squamous cell carcinoma |
| topic | Laryngeal squamous cell carcinoma Bioinformatics BIRC5 Prognosis Cell apoptosis |
| url | https://peerj.com/articles/12871.pdf |
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