Emergence of Escherichia coli, Co-Producing NDM-1 and OXA-48 Carbapenemases, in Urinary Isolates, at a Tertiary Care Centre at Central India
Objective: To detect genes encoding carbapenem resistance in urinary isolates of Escherichia coli recovered from hospitalized patients in tertiary care centre in Pune, India. Materials and Methods: From Jan 2012 to Dec 2012, a total of 300 consecutive non-duplicate (one isolate per patient) clin...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2014-06-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/4413/7952_CE(RA)_PF1(AG)_F(T)_PFA(AK)_PF2(AGAK)_PF2(PN).pdf |
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| author | Atul Khajuria Ashok Kumar Praharaj Mahadevan Kumar Naveen Grover |
| author_facet | Atul Khajuria Ashok Kumar Praharaj Mahadevan Kumar Naveen Grover |
| author_sort | Atul Khajuria |
| collection | DOAJ |
| description | Objective: To detect genes encoding carbapenem resistance in
urinary isolates of Escherichia coli recovered from hospitalized
patients in tertiary care centre in Pune, India.
Materials and Methods: From Jan 2012 to Dec 2012, a total of
300 consecutive non-duplicate (one isolate per patient) clinical
isolates of Escherichia coli were recovered from urine cultures
of hospitalized patients including hospital acquired infection
cases admitted to the medical and surgical intensive care units.
Polymerase chain reaction (PCR) assays and sequencing was
used to determine the presence of beta-lactamase encoding
genes. Conjugation experiments were performed to determine
the transferability of beta-lactamase.
Results: All the isolates were completely resistant to the
second and third generation cephalosporins tested as well as
carbapenems. All the isolates showed 100% susceptibility to
tigecycline and colistin in vitro. Conjugation experiments demonstrated that blaNDM-1 was transferable via plasmid. All the isolates
showed presence of blaNDM-1 and co-association of blaOXA-48 was
25/45(55%) of the isolates. Repetitive element based PCR (REP
PCR), Enterobacterial Repetitive Intergenic Consensus (ERIC
PCR) and Randomly Amplified Polymorphic DNA (RAPD) revealed
a diversity of six clonal types among E.coli isolates.
Conclusion: Co-production of NDM-1with OXA-48 in urinary
isolates of E. coli was detected for the first time in India.
Transmission of plasmid carrying these resistant genes to other
members of Enterobacteriaceae will increase incidence of
multidrug resistance. Early detection of these genes will help in
prevention and adequate infection control by limiting the spread
of these organisms. |
| first_indexed | 2024-12-14T15:07:55Z |
| format | Article |
| id | doaj.art-70717235c3e44bb98f3d6a9805699b9e |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-14T15:07:55Z |
| publishDate | 2014-06-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-70717235c3e44bb98f3d6a9805699b9e2022-12-21T22:56:40ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2014-06-0186DC01DC0410.7860/JCDR/2014/7952.4413Emergence of Escherichia coli, Co-Producing NDM-1 and OXA-48 Carbapenemases, in Urinary Isolates, at a Tertiary Care Centre at Central IndiaAtul Khajuria0Ashok Kumar Praharaj1Mahadevan Kumar2Naveen Grover3PhD Scholar, Department of Microbiology, Armed Forces Medical College Pune, India.PhD Scholar, Department of Microbiology, Armed Forces Medical College Pune, India.Post Graduate, Department of Microbiology, Armed Forces Medical College Pune, India.Post Graduate, Department of Microbiology, Armed Forces Medical College Pune, India.Objective: To detect genes encoding carbapenem resistance in urinary isolates of Escherichia coli recovered from hospitalized patients in tertiary care centre in Pune, India. Materials and Methods: From Jan 2012 to Dec 2012, a total of 300 consecutive non-duplicate (one isolate per patient) clinical isolates of Escherichia coli were recovered from urine cultures of hospitalized patients including hospital acquired infection cases admitted to the medical and surgical intensive care units. Polymerase chain reaction (PCR) assays and sequencing was used to determine the presence of beta-lactamase encoding genes. Conjugation experiments were performed to determine the transferability of beta-lactamase. Results: All the isolates were completely resistant to the second and third generation cephalosporins tested as well as carbapenems. All the isolates showed 100% susceptibility to tigecycline and colistin in vitro. Conjugation experiments demonstrated that blaNDM-1 was transferable via plasmid. All the isolates showed presence of blaNDM-1 and co-association of blaOXA-48 was 25/45(55%) of the isolates. Repetitive element based PCR (REP PCR), Enterobacterial Repetitive Intergenic Consensus (ERIC PCR) and Randomly Amplified Polymorphic DNA (RAPD) revealed a diversity of six clonal types among E.coli isolates. Conclusion: Co-production of NDM-1with OXA-48 in urinary isolates of E. coli was detected for the first time in India. Transmission of plasmid carrying these resistant genes to other members of Enterobacteriaceae will increase incidence of multidrug resistance. Early detection of these genes will help in prevention and adequate infection control by limiting the spread of these organisms.https://jcdr.net/articles/PDF/4413/7952_CE(RA)_PF1(AG)_F(T)_PFA(AK)_PF2(AGAK)_PF2(PN).pdfmultidrug resistantcarbapenem resistanceconjugationbeta-lactamaseuropathogenic e.coli |
| spellingShingle | Atul Khajuria Ashok Kumar Praharaj Mahadevan Kumar Naveen Grover Emergence of Escherichia coli, Co-Producing NDM-1 and OXA-48 Carbapenemases, in Urinary Isolates, at a Tertiary Care Centre at Central India Journal of Clinical and Diagnostic Research multidrug resistant carbapenem resistance conjugation beta-lactamase uropathogenic e.coli |
| title | Emergence of Escherichia coli, Co-Producing NDM-1 and OXA-48 Carbapenemases, in Urinary Isolates, at a Tertiary Care Centre at Central India |
| title_full | Emergence of Escherichia coli, Co-Producing NDM-1 and OXA-48 Carbapenemases, in Urinary Isolates, at a Tertiary Care Centre at Central India |
| title_fullStr | Emergence of Escherichia coli, Co-Producing NDM-1 and OXA-48 Carbapenemases, in Urinary Isolates, at a Tertiary Care Centre at Central India |
| title_full_unstemmed | Emergence of Escherichia coli, Co-Producing NDM-1 and OXA-48 Carbapenemases, in Urinary Isolates, at a Tertiary Care Centre at Central India |
| title_short | Emergence of Escherichia coli, Co-Producing NDM-1 and OXA-48 Carbapenemases, in Urinary Isolates, at a Tertiary Care Centre at Central India |
| title_sort | emergence of escherichia coli co producing ndm 1 and oxa 48 carbapenemases in urinary isolates at a tertiary care centre at central india |
| topic | multidrug resistant carbapenem resistance conjugation beta-lactamase uropathogenic e.coli |
| url | https://jcdr.net/articles/PDF/4413/7952_CE(RA)_PF1(AG)_F(T)_PFA(AK)_PF2(AGAK)_PF2(PN).pdf |
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