Direct detection of Mycobacterium tuberculosis with nitrate reductase assay and microscopic observation drug susceptibility
The global increase in tuberculosis drug resistant which is a threat to its control, require low cost method of diagnosis and detection. Available conventional and molecular methods consume time, and are expensive for countries with high disease burden. Nitrate Reductase Assay (NRA) and Microscopic...
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| Format: | Article |
| Language: | English |
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TMKarpinski Publisher, Tomasz M. Karpiński
2020-11-01
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| Series: | European Journal of Biological Research |
| Subjects: | |
| Online Access: | http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/338 |
| _version_ | 1819226288388308992 |
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| author | Olutayo Israel Falodun Idowu Simeon Cadmus Obasola Ezekiel Fagade |
| author_facet | Olutayo Israel Falodun Idowu Simeon Cadmus Obasola Ezekiel Fagade |
| author_sort | Olutayo Israel Falodun |
| collection | DOAJ |
| description | The global increase in tuberculosis drug resistant which is a threat to its control, require low cost method of diagnosis and detection. Available conventional and molecular methods consume time, and are expensive for countries with high disease burden. Nitrate Reductase Assay (NRA) and Microscopic Observation Drug Susceptibility (MODS) performance to directly detect tuberculosis resistance to four drugs was evaluated. The NRA (liquid and solid) and MODS performance of smear-positive sputum samples were evaluated; Sensitivities and specificities were compared with Proportion Method (PM). Sensitivity and specificity of liquid NRA (LNRA) were 90% and 98% (rifampicin), 81.8% and 100% (isoniazid), 88.9% and 98.1% (streptomycin), and 57.1% and 94.4% (ethambuthol). Also, the sensitivity and specificity for solid NRA (SNRA) were 69.2% and 98.3% (rifampicin); 100% and 100% (isoniazid); 88.9% and 95.2% (streptomycin); 70% and 80.6% (ethambuthol). Moreover, For MODS, rifampicin and isoniazid sensitivity and specificity was 100%, it was 100% and 98.1% for streptomycin, and 71.4% and 98.2% for ethambuthol. At day 14, the results available for LNRA, SNRA and MODS were 93%, 68.5% and 100% respectively. The agreement between LNRA and PM was 97% (RIF, INH and SM) and 90% (EMB). For SNRA, it was 93% (RIF), 100% (INH), 94% (SM) and 89% (EMB). While for MODS, it was 100% (RIF and INH), 98% (SM) and 95% (EMB). Direct NRA and MODS are sensitive, reliable and fast for antituberculosis drug susceptibility; they have potential to effectively and reliably detect drug resistant tuberculosis in the low resource countries.
DOI: http://dx.doi.org/10.5281/zenodo.4295881 |
| first_indexed | 2024-12-23T10:23:06Z |
| format | Article |
| id | doaj.art-7075991dd0464256883d3ccbb181e3d9 |
| institution | Directory Open Access Journal |
| issn | 2449-8955 |
| language | English |
| last_indexed | 2024-12-23T10:23:06Z |
| publishDate | 2020-11-01 |
| publisher | TMKarpinski Publisher, Tomasz M. Karpiński |
| record_format | Article |
| series | European Journal of Biological Research |
| spelling | doaj.art-7075991dd0464256883d3ccbb181e3d92022-12-21T17:50:39ZengTMKarpinski Publisher, Tomasz M. KarpińskiEuropean Journal of Biological Research2449-89552020-11-01111Direct detection of Mycobacterium tuberculosis with nitrate reductase assay and microscopic observation drug susceptibilityOlutayo Israel Falodun0Idowu Simeon Cadmus1Obasola Ezekiel Fagade2Department of Microbiology, University of Ibadan, NigeriaDepartment of Veterinary Public Health and Preventive Medicine, University of Ibadan, NigeriaDepartment of Microbiology, University of Ibadan, NigeriaThe global increase in tuberculosis drug resistant which is a threat to its control, require low cost method of diagnosis and detection. Available conventional and molecular methods consume time, and are expensive for countries with high disease burden. Nitrate Reductase Assay (NRA) and Microscopic Observation Drug Susceptibility (MODS) performance to directly detect tuberculosis resistance to four drugs was evaluated. The NRA (liquid and solid) and MODS performance of smear-positive sputum samples were evaluated; Sensitivities and specificities were compared with Proportion Method (PM). Sensitivity and specificity of liquid NRA (LNRA) were 90% and 98% (rifampicin), 81.8% and 100% (isoniazid), 88.9% and 98.1% (streptomycin), and 57.1% and 94.4% (ethambuthol). Also, the sensitivity and specificity for solid NRA (SNRA) were 69.2% and 98.3% (rifampicin); 100% and 100% (isoniazid); 88.9% and 95.2% (streptomycin); 70% and 80.6% (ethambuthol). Moreover, For MODS, rifampicin and isoniazid sensitivity and specificity was 100%, it was 100% and 98.1% for streptomycin, and 71.4% and 98.2% for ethambuthol. At day 14, the results available for LNRA, SNRA and MODS were 93%, 68.5% and 100% respectively. The agreement between LNRA and PM was 97% (RIF, INH and SM) and 90% (EMB). For SNRA, it was 93% (RIF), 100% (INH), 94% (SM) and 89% (EMB). While for MODS, it was 100% (RIF and INH), 98% (SM) and 95% (EMB). Direct NRA and MODS are sensitive, reliable and fast for antituberculosis drug susceptibility; they have potential to effectively and reliably detect drug resistant tuberculosis in the low resource countries. DOI: http://dx.doi.org/10.5281/zenodo.4295881http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/338Tuberculosis drug resistanceMycobacterium detection NRAMODSDiagnosis |
| spellingShingle | Olutayo Israel Falodun Idowu Simeon Cadmus Obasola Ezekiel Fagade Direct detection of Mycobacterium tuberculosis with nitrate reductase assay and microscopic observation drug susceptibility European Journal of Biological Research Tuberculosis drug resistance Mycobacterium detection NRA MODS Diagnosis |
| title | Direct detection of Mycobacterium tuberculosis with nitrate reductase assay and microscopic observation drug susceptibility |
| title_full | Direct detection of Mycobacterium tuberculosis with nitrate reductase assay and microscopic observation drug susceptibility |
| title_fullStr | Direct detection of Mycobacterium tuberculosis with nitrate reductase assay and microscopic observation drug susceptibility |
| title_full_unstemmed | Direct detection of Mycobacterium tuberculosis with nitrate reductase assay and microscopic observation drug susceptibility |
| title_short | Direct detection of Mycobacterium tuberculosis with nitrate reductase assay and microscopic observation drug susceptibility |
| title_sort | direct detection of mycobacterium tuberculosis with nitrate reductase assay and microscopic observation drug susceptibility |
| topic | Tuberculosis drug resistance Mycobacterium detection NRA MODS Diagnosis |
| url | http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/338 |
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