Standardization of 3D printing parameters to control the size and shape of pores in Polylactic acid scaffolds
Abstract The challenge of three‐dimensional (3D) printing by polymeric extrusion in tissue bioengineering is to control with precision the microarchitecture and porous interconnectivity of scaffolds, as well as search for models that allow and facilitate the development of personalized constructs th...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2024-03-01
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| Series: | MedComm – Biomaterials and Applications |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/mba2.74 |
| _version_ | 1797257534877204480 |
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| author | Lucía Pérez‐Sánchez Misael A. Ortiz de la O Marco A. Álvarez‐Pérez Monserrat Llaguno‐Munive Osmar A. Chanes‐Cuevas Janeth Serrano‐Bello |
| author_facet | Lucía Pérez‐Sánchez Misael A. Ortiz de la O Marco A. Álvarez‐Pérez Monserrat Llaguno‐Munive Osmar A. Chanes‐Cuevas Janeth Serrano‐Bello |
| author_sort | Lucía Pérez‐Sánchez |
| collection | DOAJ |
| description | Abstract The challenge of three‐dimensional (3D) printing by polymeric extrusion in tissue bioengineering is to control with precision the microarchitecture and porous interconnectivity of scaffolds, as well as search for models that allow and facilitate the development of personalized constructs that meet optimal characteristics for the regeneration of significant bone defects. In this study, anatomically accurate scaffolds were designed and printed to a critical size defect from a microtomography image of the rat calvaria. Different software is used to design a scaffold with exact anatomy. With Ultimaker Cura software, distinct printing parameters were standardized, permitting the printing of different types of pores and graded porosity scaffolds, with exact adaptation to the bone defect, utilizing a commercial 3D printer with a fused deposition modeling technique and compensating for the limitations of the method. The scaffolds were characterized by evaluating their mechanical properties and surface characteristics (pore size and porosity), employing scanning electron microscopy images, verifying that the size and shape of the pores were controlled, and evaluating cell viability and cell distribution on the 3D printed scaffold. Therefore, this work proves that by standardizing the printing parameters, it was possible to print a unique customized scaffold, controlling the shape and size of pores. |
| first_indexed | 2024-04-24T22:39:10Z |
| format | Article |
| id | doaj.art-7076aef035084458a1294334da9036b8 |
| institution | Directory Open Access Journal |
| issn | 2769-643X |
| language | English |
| last_indexed | 2024-04-24T22:39:10Z |
| publishDate | 2024-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | MedComm – Biomaterials and Applications |
| spelling | doaj.art-7076aef035084458a1294334da9036b82024-03-19T05:24:27ZengWileyMedComm – Biomaterials and Applications2769-643X2024-03-0131n/an/a10.1002/mba2.74Standardization of 3D printing parameters to control the size and shape of pores in Polylactic acid scaffoldsLucía Pérez‐Sánchez0Misael A. Ortiz de la O1Marco A. Álvarez‐Pérez2Monserrat Llaguno‐Munive3Osmar A. Chanes‐Cuevas4Janeth Serrano‐Bello5Laboratorio de Bioingeniería de Tejidos, División de Estudios de Posgrado e Investigación, Facultad de Odontología Universidad Nacional Autónoma de México, Circuito Exterior s/n. Cd. Universitaria CDMX MexicoLaboratorio de Bioingeniería de Tejidos, División de Estudios de Posgrado e Investigación, Facultad de Odontología Universidad Nacional Autónoma de México, Circuito Exterior s/n. Cd. Universitaria CDMX MexicoLaboratorio de Bioingeniería de Tejidos, División de Estudios de Posgrado e Investigación, Facultad de Odontología Universidad Nacional Autónoma de México, Circuito Exterior s/n. Cd. Universitaria CDMX MexicoLaboratorio de Física Médica, Subdirección de Investigación Básica Instituto Nacional de Cancerología CDMX MexicoLaboratorio de Investigación de Materiales Dentales y Biomateriales, División de Estudios de Posgrado e Investigación, Facultad de Odontología Universidad Nacional Autónoma de México, Circuito Exterior s/n. Cd. Universitaria CDMX MexicoLaboratorio de Bioingeniería de Tejidos, División de Estudios de Posgrado e Investigación, Facultad de Odontología Universidad Nacional Autónoma de México, Circuito Exterior s/n. Cd. Universitaria CDMX MexicoAbstract The challenge of three‐dimensional (3D) printing by polymeric extrusion in tissue bioengineering is to control with precision the microarchitecture and porous interconnectivity of scaffolds, as well as search for models that allow and facilitate the development of personalized constructs that meet optimal characteristics for the regeneration of significant bone defects. In this study, anatomically accurate scaffolds were designed and printed to a critical size defect from a microtomography image of the rat calvaria. Different software is used to design a scaffold with exact anatomy. With Ultimaker Cura software, distinct printing parameters were standardized, permitting the printing of different types of pores and graded porosity scaffolds, with exact adaptation to the bone defect, utilizing a commercial 3D printer with a fused deposition modeling technique and compensating for the limitations of the method. The scaffolds were characterized by evaluating their mechanical properties and surface characteristics (pore size and porosity), employing scanning electron microscopy images, verifying that the size and shape of the pores were controlled, and evaluating cell viability and cell distribution on the 3D printed scaffold. Therefore, this work proves that by standardizing the printing parameters, it was possible to print a unique customized scaffold, controlling the shape and size of pores.https://doi.org/10.1002/mba2.743D printinggraded porosityprinting parametersscaffolding |
| spellingShingle | Lucía Pérez‐Sánchez Misael A. Ortiz de la O Marco A. Álvarez‐Pérez Monserrat Llaguno‐Munive Osmar A. Chanes‐Cuevas Janeth Serrano‐Bello Standardization of 3D printing parameters to control the size and shape of pores in Polylactic acid scaffolds MedComm – Biomaterials and Applications 3D printing graded porosity printing parameters scaffolding |
| title | Standardization of 3D printing parameters to control the size and shape of pores in Polylactic acid scaffolds |
| title_full | Standardization of 3D printing parameters to control the size and shape of pores in Polylactic acid scaffolds |
| title_fullStr | Standardization of 3D printing parameters to control the size and shape of pores in Polylactic acid scaffolds |
| title_full_unstemmed | Standardization of 3D printing parameters to control the size and shape of pores in Polylactic acid scaffolds |
| title_short | Standardization of 3D printing parameters to control the size and shape of pores in Polylactic acid scaffolds |
| title_sort | standardization of 3d printing parameters to control the size and shape of pores in polylactic acid scaffolds |
| topic | 3D printing graded porosity printing parameters scaffolding |
| url | https://doi.org/10.1002/mba2.74 |
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