Identification of Two Novel Peanut Genotypes Resistant to Aflatoxin Production and Their SNP Markers Associated with Resistance
Aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) and aflatoxin B<sub>2</sub> (AFB<sub>2</sub>) are the most common aflatoxins produced by <i>Aspergillus flavus</i> in peanuts, with high carcinogenicity and teratogenicity. Identification of DNA mar...
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MDPI AG
2020-03-01
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| Series: | Toxins |
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| author | Bolun Yu Huifang Jiang Manish K. Pandey Li Huang Dongxin Huai Xiaojing Zhou Yanping Kang Rajeev K. Varshney Hari K. Sudini Xiaoping Ren Huaiyong Luo Nian Liu Weigang Chen Jianbin Guo Weitao Li Yingbin Ding Yifei Jiang Yong Lei Boshou Liao |
| author_facet | Bolun Yu Huifang Jiang Manish K. Pandey Li Huang Dongxin Huai Xiaojing Zhou Yanping Kang Rajeev K. Varshney Hari K. Sudini Xiaoping Ren Huaiyong Luo Nian Liu Weigang Chen Jianbin Guo Weitao Li Yingbin Ding Yifei Jiang Yong Lei Boshou Liao |
| author_sort | Bolun Yu |
| collection | DOAJ |
| description | Aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) and aflatoxin B<sub>2</sub> (AFB<sub>2</sub>) are the most common aflatoxins produced by <i>Aspergillus flavus</i> in peanuts, with high carcinogenicity and teratogenicity. Identification of DNA markers associated with resistance to aflatoxin production is likely to offer breeders efficient tools to develop resistant cultivars through molecular breeding. In this study, seeds of 99 accessions of a Chinese peanut mini-mini core collection were investigated for their reaction to aflatoxin production by a laboratory kernel inoculation assay. Two resistant accessions (Zh.h0551 and Zh.h2150) were identified, with their aflatoxin content being 8.11%−18.90% of the susceptible control. The 99 peanut accessions were also genotyped by restriction site-associated DNA sequencing (RAD-Seq) for a genome-wide association study (GWAS). A total of 60 SNP (single nucleotide polymorphism) markers associated with aflatoxin production were detected, and they explained 16.87%−31.70% of phenotypic variation (PVE), with SNP02686 and SNP19994 possessing 31.70% and 28.91% PVE, respectively. Aflatoxin contents of accessions with “AG” (existed in Zh.h0551 and Zh.h2150) and “GG” genotypes of either SNP19994 or SNP02686 were significantly lower than that of “AA” genotypes in the mean value of a three-year assay. The resistant accessions and molecular markers identified in this study are likely to be helpful for deployment in aflatoxin resistance breeding in peanuts. |
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| language | English |
| last_indexed | 2024-04-11T14:09:18Z |
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| spelling | doaj.art-70772459a0d34ea890e10bec5ccdcb592022-12-22T04:19:46ZengMDPI AGToxins2072-66512020-03-0112315610.3390/toxins12030156toxins12030156Identification of Two Novel Peanut Genotypes Resistant to Aflatoxin Production and Their SNP Markers Associated with ResistanceBolun Yu0Huifang Jiang1Manish K. Pandey2Li Huang3Dongxin Huai4Xiaojing Zhou5Yanping Kang6Rajeev K. Varshney7Hari K. Sudini8Xiaoping Ren9Huaiyong Luo10Nian Liu11Weigang Chen12Jianbin Guo13Weitao Li14Yingbin Ding15Yifei Jiang16Yong Lei17Boshou Liao18Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crop Research Institute (OCRI) of Chinese Academy of Agricultural Sciences (CAAS), Wuhan 430062, ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crop Research Institute (OCRI) of Chinese Academy of Agricultural Sciences (CAAS), Wuhan 430062, ChinaInternational Crops Research Institute of the Semi-Arid Tropics (ICRISAT), Hyderabad 502324, IndiaKey Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crop Research Institute (OCRI) of Chinese Academy of Agricultural Sciences (CAAS), Wuhan 430062, ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crop Research Institute (OCRI) of Chinese Academy of Agricultural Sciences (CAAS), Wuhan 430062, ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crop Research Institute (OCRI) of Chinese Academy of Agricultural Sciences (CAAS), Wuhan 430062, ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crop Research Institute (OCRI) of Chinese Academy of Agricultural Sciences (CAAS), Wuhan 430062, ChinaInternational Crops Research Institute of the Semi-Arid Tropics (ICRISAT), Hyderabad 502324, IndiaInternational Crops Research Institute of the Semi-Arid Tropics (ICRISAT), Hyderabad 502324, IndiaKey Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crop Research Institute (OCRI) of Chinese Academy of Agricultural Sciences (CAAS), Wuhan 430062, ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crop Research Institute (OCRI) of Chinese Academy of Agricultural Sciences (CAAS), Wuhan 430062, ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crop Research Institute (OCRI) of Chinese Academy of Agricultural Sciences (CAAS), Wuhan 430062, ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crop Research Institute (OCRI) of Chinese Academy of Agricultural Sciences (CAAS), Wuhan 430062, ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crop Research Institute (OCRI) of Chinese Academy of Agricultural Sciences (CAAS), Wuhan 430062, ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crop Research Institute (OCRI) of Chinese Academy of Agricultural Sciences (CAAS), Wuhan 430062, ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crop Research Institute (OCRI) of Chinese Academy of Agricultural Sciences (CAAS), Wuhan 430062, ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crop Research Institute (OCRI) of Chinese Academy of Agricultural Sciences (CAAS), Wuhan 430062, ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crop Research Institute (OCRI) of Chinese Academy of Agricultural Sciences (CAAS), Wuhan 430062, ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crop Research Institute (OCRI) of Chinese Academy of Agricultural Sciences (CAAS), Wuhan 430062, ChinaAflatoxin B<sub>1</sub> (AFB<sub>1</sub>) and aflatoxin B<sub>2</sub> (AFB<sub>2</sub>) are the most common aflatoxins produced by <i>Aspergillus flavus</i> in peanuts, with high carcinogenicity and teratogenicity. Identification of DNA markers associated with resistance to aflatoxin production is likely to offer breeders efficient tools to develop resistant cultivars through molecular breeding. In this study, seeds of 99 accessions of a Chinese peanut mini-mini core collection were investigated for their reaction to aflatoxin production by a laboratory kernel inoculation assay. Two resistant accessions (Zh.h0551 and Zh.h2150) were identified, with their aflatoxin content being 8.11%−18.90% of the susceptible control. The 99 peanut accessions were also genotyped by restriction site-associated DNA sequencing (RAD-Seq) for a genome-wide association study (GWAS). A total of 60 SNP (single nucleotide polymorphism) markers associated with aflatoxin production were detected, and they explained 16.87%−31.70% of phenotypic variation (PVE), with SNP02686 and SNP19994 possessing 31.70% and 28.91% PVE, respectively. Aflatoxin contents of accessions with “AG” (existed in Zh.h0551 and Zh.h2150) and “GG” genotypes of either SNP19994 or SNP02686 were significantly lower than that of “AA” genotypes in the mean value of a three-year assay. The resistant accessions and molecular markers identified in this study are likely to be helpful for deployment in aflatoxin resistance breeding in peanuts.https://www.mdpi.com/2072-6651/12/3/156aflatoxin resistancepeanutgenome-wide association study (gwas)mini-mini core collectionrestriction site associated dna sequencing (rad-seq) |
| spellingShingle | Bolun Yu Huifang Jiang Manish K. Pandey Li Huang Dongxin Huai Xiaojing Zhou Yanping Kang Rajeev K. Varshney Hari K. Sudini Xiaoping Ren Huaiyong Luo Nian Liu Weigang Chen Jianbin Guo Weitao Li Yingbin Ding Yifei Jiang Yong Lei Boshou Liao Identification of Two Novel Peanut Genotypes Resistant to Aflatoxin Production and Their SNP Markers Associated with Resistance Toxins aflatoxin resistance peanut genome-wide association study (gwas) mini-mini core collection restriction site associated dna sequencing (rad-seq) |
| title | Identification of Two Novel Peanut Genotypes Resistant to Aflatoxin Production and Their SNP Markers Associated with Resistance |
| title_full | Identification of Two Novel Peanut Genotypes Resistant to Aflatoxin Production and Their SNP Markers Associated with Resistance |
| title_fullStr | Identification of Two Novel Peanut Genotypes Resistant to Aflatoxin Production and Their SNP Markers Associated with Resistance |
| title_full_unstemmed | Identification of Two Novel Peanut Genotypes Resistant to Aflatoxin Production and Their SNP Markers Associated with Resistance |
| title_short | Identification of Two Novel Peanut Genotypes Resistant to Aflatoxin Production and Their SNP Markers Associated with Resistance |
| title_sort | identification of two novel peanut genotypes resistant to aflatoxin production and their snp markers associated with resistance |
| topic | aflatoxin resistance peanut genome-wide association study (gwas) mini-mini core collection restriction site associated dna sequencing (rad-seq) |
| url | https://www.mdpi.com/2072-6651/12/3/156 |
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